scholarly journals High-Throughput Identification of Catalytic Redox-Active Cysteine Residues

Science ◽  
2007 ◽  
Vol 315 (5810) ◽  
pp. 387-389 ◽  
Author(s):  
D. E. Fomenko ◽  
W. Xing ◽  
B. M. Adair ◽  
D. J. Thomas ◽  
V. N. Gladyshev
Keyword(s):  
High Throughput ◽  
Cysteine Residues ◽  
Redox Active ◽  
Redox Active Cysteine

scholarly journals Novel Oxidative Modifications in Redox-Active Cysteine Residues

2010 ◽  
Vol 10 (3) ◽  
pp. M110.000513 ◽  
Author(s):  
Jaeho Jeong ◽  
Yongsik Jung ◽  
Seungjin Na ◽  
Jihye Jeong ◽  
Eunsun Lee ◽  
...  
Keyword(s):  
Cysteine Residues ◽  
Redox Active ◽  
Oxidative Modifications ◽  
Redox Active Cysteine

scholarly journals A “Seleno Effect” Differentiates the Roles of Redox Active Cysteine Residues in Plasmodium falciparum Thioredoxin Reductase

Biochemistry ◽  
2018 ◽  
Vol 57 (11) ◽  
pp. 1767-1778 ◽  
Author(s):  
John P. O’Keefe ◽  
Christopher M. Dustin ◽  
Drew Barber ◽  
Gregg W. Snider ◽  
Robert J. Hondal
Keyword(s):  
Plasmodium Falciparum ◽  
Thioredoxin Reductase ◽  
Cysteine Residues ◽  
Redox Active ◽  
Redox Active Cysteine

scholarly journals Computational design of molecules for an all-quinone redox flow battery

2015 ◽  
Vol 6 (2) ◽  
pp. 885-893 ◽  
Author(s):  
Süleyman Er ◽  
Changwon Suh ◽  
Michael P. Marshak ◽  
Alán Aspuru-Guzik
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Computational Design ◽  
Redox Flow Battery ◽  
Flow Battery ◽  
Screening Approach ◽  
Redox Active ◽  
Flow Batteries ◽  
Aqueous Flow

We demonstrate a successful high-throughput screening approach for the discovery of inexpensive, redox-active quinone molecules for organic-based aqueous flow batteries.

scholarly journals Accelerated corrosion of marine-grade steel by a redox-active, cysteine-rich barnacle cement protein

2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Vinod K. Murugan ◽  
Harini Mohanram ◽  
Maja Budanovic ◽  
Arvind Latchou ◽  
Richard David Webster ◽  
...  
Keyword(s):  
Accelerated Corrosion ◽  
Redox Active ◽  
Cement Protein ◽  
Grade Steel ◽  
Barnacle Cement ◽  
Redox Active Cysteine

scholarly journals A High-Throughput Mass Spectrometry Assay Coupled with Redox Activity Testing Reduces Artifacts and False Positives in Lysine Demethylase Screening

2015 ◽  
Vol 20 (6) ◽  
pp. 810-820 ◽  
Author(s):  
Tim J. Wigle ◽  
Kerren K. Swinger ◽  
John E. Campbell ◽  
Michael D. Scholle ◽  
John Sherrill ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
High Throughput Screening ◽  
Critical Role ◽  
Biological Significance ◽  
Redox Activity ◽  
Self Assembled Monolayer ◽  
Redox Active ◽  
Oxidation Chemistry ◽  
Lysine Demethylase

Demethylation of histones by lysine demethylases (KDMs) plays a critical role in controlling gene transcription. Aberrant demethylation may play a causal role in diseases such as cancer. Despite the biological significance of these enzymes, there are limited assay technologies for study of KDMs and few quality chemical probes available to interrogate their biology. In this report, we demonstrate the utility of self-assembled monolayer desorption/ionization (SAMDI) mass spectrometry for the investigation of quantitative KDM enzyme kinetics and for high-throughput screening for KDM inhibitors. SAMDI can be performed in 384-well format and rapidly allows reaction components to be purified prior to injection into a mass spectrometer, without a throughput-limiting liquid chromatography step. We developed sensitive and robust assays for KDM1A (LSD1, AOF2) and KDM4C (JMJD2C, GASC1) and screened 13,824 compounds against each enzyme. Hits were rapidly triaged using a redox assay to identify compounds that interfered with the catalytic oxidation chemistry used by the KDMs for the demethylation reaction. We find that overall this high-throughput mass spectrometry platform coupled with the elimination of redox active compounds leads to a hit rate that is manageable for follow-up work.

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