Single-Neuron Activity and Tissue Oxygenation in the Cerebral Cortex

Science ◽  
2003 ◽  
Vol 299 (5609) ◽  
pp. 1070-1072 ◽  
Author(s):  
J. K. Thompson
Keyword(s):  
Cerebral Cortex ◽  
Single Neuron ◽  
Tissue Oxygenation ◽  
Neuron Activity ◽  
Single Neuron Activity

In vivo visualization of single-unit recording sites using MRI-detectable elgiloy deposit marking

2011 ◽  
Vol 105 (3) ◽  
pp. 1380-1392 ◽  
Author(s):  
Kenji W. Koyano ◽  
Akinori Machino ◽  
Masaki Takeda ◽  
Teppei Matsui ◽  
Ryoko Fujimichi ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Single Neuron ◽  
Cognitive Task ◽  
Neuron Activity ◽  
Electrolytic Lesion ◽  
Mr Images ◽  
Gold Standard Method ◽  
Spin Echo Sequence ◽  
Single Neuron Activity

Precise localization of single-neuron activity has elucidated functional architectures of the primate cerebral cortex, related to vertically stacked layers and horizontally aligned columns. The traditional “gold standard” method for localizing recorded neuron is histological examination of electrolytic lesion marks at recording sites. Although this method can localize recorded neurons with fine neuroanatomy, the necessity for postmortem analysis prohibits its use in long-term chronic experiments. To localize recorded single-neuron positions in vivo, we introduced MRI-detectable elgiloy deposit marks, which can be created by electrolysis of an elgiloy microelectrode tip and visualized on highly contrasted magnetic resonance (MR) images. Histological analysis validated that the deposit mark centers could be localized relative to neuroanatomy in vivo with single-voxel accuracy, at an in-plane resolution of 200 μm. To demonstrate practical applications of the technique, we recorded single-neuron activity from a monkey performing a cognitive task and localized it in vivo using deposit marks (deposition: 2 μA for 3 min; scanning: fast-spin-echo sequence with 0.15 × 0.15 × 0.8 mm3 resolution, 120/4,500 ms of echo-time/repetition-time and 8 echo-train-length), as is usually performed with conventional postmortem methods using electrolytic lesion marks. Two localization procedures were demonstrated: 1) deposit marks within a microelectrode track were used to reconstruct a dozen recorded neuron positions along the track directly on MR images; 2) combination with X-ray imaging allowed estimation of hundreds of neuron positions on MR images. This new in vivo method is feasible for chronic experiments with nonhuman primates, enabling analysis of the functional architecture of the cerebral cortex underlying cognitive processes.

Transcutaneous recording of single neuron activity in the cerebral cortex of the monkey

1983 ◽  
Vol 9 (3) ◽  
pp. 253-257
Author(s):  
Ian Darian-Smith ◽  
Geoffrey Durham-Smith ◽  
Michio Sugitani ◽  
John Heywood ◽  
Antony Goodwin
Keyword(s):  
Cerebral Cortex ◽  
Single Neuron ◽  
Neuron Activity ◽  
Single Neuron Activity
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