Extent of Chromatin Spreading Determined by roX RNA Recruitment of MSL Proteins

Science ◽  
2002 ◽  
Vol 298 (5598) ◽  
pp. 1620-1623 ◽  
Author(s):  
Y. Park
Keyword(s):  
Rox Rna

Drosophila Male-Specific Lethal 2 Protein Controls Sex-Specific Expression of the roX Genes

Genetics ◽  
2004 ◽  
Vol 166 (4) ◽  
pp. 1825-1832 ◽  
Author(s):  
Barbara P Rattner ◽  
Victoria H Meller
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
X Chromosome ◽  
Specific Expression ◽  
Linked Gene ◽  
Male And Female ◽  
Male Specific Lethal ◽  
Msl Complex ◽  
Male Specific ◽  
Rox Rna

Abstract The MSL complex of Drosophila upregulates transcription of the male X chromosome, equalizing male and female X-linked gene expression. Five male-specific lethal proteins and at least one of the two noncoding roX RNAs are essential for this process. The roX RNAs are required for the localization of MSL complexes to the X chromosome. Although the mechanisms directing targeting remain speculative, the ratio of MSL protein to roX RNA influences localization of the complex. We examine the transcriptional regulation of the roX genes and show that MSL2 controls male-specific roX expression in the absence of any other MSL protein. We propose that this mechanism maintains a stable MSL/roX ratio that is favorable for localization of the complex to the X chromosome.

scholarly journals Structure, dynamics and roX2-lncRNA binding of tandem double-stranded RNA binding domains dsRBD1,2 of Drosophila helicase Maleless

2018 ◽  
Author(s):  
Pravin Kumar Ankush Jagtap ◽  
Marisa Müller ◽  
Pawel Masiewicz ◽  
Sören von Bülow ◽  
Nele Merret Hollmann ◽  
...  
Keyword(s):  
Rna Binding ◽  
Binding Motifs ◽  
Double Stranded Rna ◽  
Binding Domains ◽  
Dsrna Binding ◽  
Rna Binding Domains ◽  
Human Orthologue ◽  
Rox Rna

ABSTRACTMaleless (MLE) is an evolutionary conserved member of the DExH family of helicases in Drosophila. Besides its function in RNA editing and presumably siRNA processing, MLE is best known for its role in remodelling non-coding roX RNA in the context of X chromosome dosage compensation in male flies. MLE and its human orthologue, DHX9 contain two tandem double-stranded RNA binding domains (dsRBDs) located at the N-terminal region. The two dsRBDs are essential for localization of MLE at the X-territory and it is presumed that this involves binding roX secondary structures. However, for dsRBD1 roX RNA binding has so far not been described. Here, we determined the solution NMR structure of dsRBD1 and dsRBD2 of MLE in tandem and investigated its role in double-stranded RNA (dsRNA) binding. Our NMR data show that both dsRBDs act as independent structural modules in solution and are canonical, non-sequence-specific dsRBDs featuring non-canonical KKxAK RNA binding motifs. NMR titrations combined with filter binding experiments document the contribution of dsRBD1 to dsRNA binding in vitro. Curiously, dsRBD1 mutants in which dsRNA binding in vitro is strongly compromised do not affect roX2 RNA binding and MLE localization in cells. These data suggest alternative functions for dsRBD1 in vivo.

scholarly journals Sequence-specific targeting of MSL complex regulates transcription of the roX RNA genes

2004 ◽  
Vol 23 (14) ◽  
pp. 2853-2861 ◽  
Author(s):  
Xiaoying Bai ◽  
Artyom A Alekseyenko ◽  
Mitzi I Kuroda
Keyword(s):  
Specific Targeting ◽  
Msl Complex ◽  
Rna Genes ◽  
Rox Rna

scholarly journals Epigenetic Spreading of the Drosophila Dosage Compensation Complex from roX RNA Genes into Flanking Chromatin

Cell ◽  
1999 ◽  
Vol 98 (4) ◽  
pp. 513-522 ◽  
Author(s):  
Richard L Kelley ◽  
Victoria H Meller ◽  
Polina R Gordadze ◽  
Gregg Roman ◽  
Ronald L Davis ◽  
...  
Keyword(s):  
Dosage Compensation ◽  
Dosage Compensation Complex ◽  
Rna Genes ◽  
Rox Rna

scholarly journals ATP-Dependent roX RNA Remodeling by the Helicase maleless Enables Specific Association of MSL Proteins

2013 ◽  
Vol 51 (2) ◽  
pp. 174-184 ◽  
Author(s):  
Sylvain Maenner ◽  
Marisa Müller ◽  
Jonathan Fröhlich ◽  
Diana Langer ◽  
Peter B. Becker
Keyword(s):  
Rna Remodeling ◽  
Specific Association ◽  
Rox Rna
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