scholarly journals Advanced PLGA hybrid scaffold with a bioactive PDRN/BMP2 nanocomplex for angiogenesis and bone regeneration using human fetal MSCs

2021 ◽  
Vol 7 (50) ◽  
Author(s):  
Da-Seul Kim ◽  
Jun-Kyu Lee ◽  
Jun Hyuk Kim ◽  
Jaemin Lee ◽  
Dong Seon Kim ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Hybrid Scaffold

scholarly journals Smoothened agonist sterosome immobilized hybrid scaffold for bone regeneration

2020 ◽  
Vol 6 (17) ◽  
pp. eaaz7822 ◽  
Author(s):  
Chung-Sung Lee ◽  
Soyon Kim ◽  
Jiabing Fan ◽  
Hee Sook Hwang ◽  
Tara Aghaloo ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Cell Fate ◽  
Bone Repair ◽  
Drug Loading ◽  
Three Dimensional ◽  
Hedgehog Pathway ◽  
Hybrid Scaffold ◽  
Synergistic Activation ◽  
Bioactive Agents ◽  
Osteoinductive Property

Biomaterial delivery of bioactive agents and manipulation of stem cell fate are an attractive approach to promote tissue regeneration. Here, smoothened agonist sterosome is developed using small-molecule activators [20S-hydroxycholesterol (OHC) and purmorphamine (PUR)] of the smoothened protein in the hedgehog pathway as carrier and cargo. Sterosome presents inherent osteoinductive property even without drug loading. Sterosome is covalently immobilized onto three-dimensional scaffolds via a bioinspired polydopamine intermediate to fabricate a hybrid scaffold for bone regeneration. Sterosome-immobilized hybrid scaffold not only provides a favorable substrate for cell adhesion and proliferation but also delivers bioactive agents in a sustained and spatially targeted manner. Furthermore, this scaffold significantly improves osteogenic differentiation of bone marrow stem cells through OHC/PUR-mediated synergistic activation of the hedgehog pathway and also enhances bone repair in a mouse calvarial defect model. This system serves as a versatile biomaterial platform for many applications, including therapeutic delivery and endogenous regenerative medicine.

scholarly journals Application of a New Type of Natural Calcined Bone Repair Material Combined with Concentrated Growth Factors in Bone Regeneration in Rabbit Critical-Sized Calvarial Defect

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Xiaoyang Wang ◽  
Shuqing Tong ◽  
Shengyun Huang ◽  
Li Ma ◽  
Zhenxing Liu ◽  
...  
Keyword(s):  
Growth Factors ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Bone Repair ◽  
Blank Group ◽  
Male Adult ◽  
Animal Group ◽  
Hybrid Scaffold ◽  
Concentrated Growth Factors ◽  
The Right

Purpose. This study is aimed at investigating bone regeneration in critical-sized defects in rabbit calvarium using a novel nano- (n-) hydroxyapatite hybrid scaffold with concentrated growth factors (CGFs). Methods. Twenty-four male adult rabbits were chosen to establish a critical-sized bone defect model and randomly divided into two groups. Two defects of 15 mm diameter each were created in the parietal bone of each animal. Group A had n-hydroxyapatite hybrid scaffold placed in the experimental defect on the right, and the left defect was unfilled as blank. Group B had hydroxyapatite hybrid scaffold mixed with CGF placed in the right defect and CGF on the left. Six animals in each group were sacrificed after 6 and 12 weeks. Cone-beam computed tomography system scanning and hematoxylin and eosin (HE) staining were used to detect osteogenesis within the defects. Results. The treatment with n-hydroxyapatite hybrid scaffold along with CGF resulted in a significantly higher amount of new bone at 6 and 12 weeks compared to the treatment with CGF alone and the controls. No apparent inflammation and foreign body reaction were observed through HE staining. Conclusions. The new synthesized n-hydroxyapatite hybrid scaffold and CGF can be applied for bone defect regeneration to promote the process to a certain extent.

scholarly journals Mechanically Strong Silica-Silk Fibroin Bioaerogel: A Hybrid Scaffold with Ordered Honeycomb Micromorphology and Multiscale Porosity for Bone Regeneration

2019 ◽  
Vol 11 (19) ◽  
pp. 17256-17269 ◽  
Author(s):  
Hajar Maleki ◽  
Mohammad-Ali Shahbazi ◽  
Susan Montes ◽  
Seyed Hojjat Hosseini ◽  
Mohammad Reza Eskandari ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Silk Fibroin ◽  
Hybrid Scaffold

scholarly journals An ECM-Mimicking, Mesenchymal Stem Cell-Embedded Hybrid Scaffold for Bone Regeneration

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Jozafina Haj ◽  
Tharwat Haj Khalil ◽  
Mizied Falah ◽  
Eyal Zussman ◽  
Samer Srouji
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Repair ◽  
Dorsal Side ◽  
Human Mscs ◽  
Hybrid Scaffold

While biologically feasible, bone repair is often inadequate, particularly in cases of large defects. The search for effective bone regeneration strategies has led to the emergence of bone tissue engineering (TE) techniques. When integrating electrospinning techniques, scaffolds featuring randomly oriented or aligned fibers, characteristic of the extracellular matrix (ECM), can be fabricated. In parallel, mesenchymal stem cells (MSCs), which are capable of both self-renewing and differentiating into numerous tissue types, have been suggested to be a suitable option for cell-based tissue engineering therapies. This work aimed to create a novel biocompatible hybrid scaffold composed of electrospun polymeric nanofibers combined with osteoconductive ceramics, loaded with human MSCs, to yield a tissue-like construct to promote in vivo bone formation. Characterization of the cell-embedded scaffolds demonstrated their resemblance to bone tissue extracellular matrix, on both micro- and nanoscales and MSC viability and integration within the electrospun nanofibers. Subcutaneous implantation of the cell-embedded scaffolds in the dorsal side of mice led to new bone, muscle, adipose, and connective tissue formation within 8 weeks. This hybrid scaffold may represent a step forward in the pursuit of advanced bone tissue engineering scaffolds.

A conceptually new type of bio-hybrid scaffold for bone regeneration

2010 ◽  
Vol 22 (1) ◽  
pp. 015104 ◽  
Author(s):  
A Tampieri ◽  
E Landi ◽  
F Valentini ◽  
M Sandri ◽  
T D’Alessandro ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Hybrid Scaffold ◽  
New Type

scholarly journals Fabrication of Three-Dimensional Composite Scaffold for Simultaneous Alveolar Bone Regeneration in Dental Implant Installation

2020 ◽  
Vol 21 (5) ◽  
pp. 1863 ◽  
Author(s):  
Hun-Jin Jeong ◽  
So-Jung Gwak ◽  
Kyoung Duck Seo ◽  
SaYa Lee ◽  
Jeong-Ho Yun ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Regeneration ◽  
Dental Implant ◽  
Alveolar Bone ◽  
Insertion Site ◽  
High Porosity ◽  
Hybrid Scaffold ◽  
Implant Surgery ◽  
3D Printed ◽  
Dental Implant Surgery

Dental implant surgeries involve the insertion of implant fixtures into alveolar bones to replace missing teeth. When the availability of alveolar bone at the surgical site is insufficient, bone graft particles are filled in the insertion site for successful bone reconstruction. Bone graft particles induce bone regeneration over several months at the insertion site. Subsequently, implant fixtures can be inserted at the recipient site. Thus, conventional dental implant surgery is performed in several steps, which in turn increases the treatment period and cost involved. Therefore, to reduce surgical time and minimize treatment costs, a novel hybrid scaffold filled with bone graft particles that could be combined with implant fixtures is proposed. This scaffold is composed of a three-dimensionally (3D) printed polycaprolactone (PCL) frame and osteoconductive ceramic materials such as hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP). Herein, we analyzed the porosity, internal microstructure, and hydrophilicity of the hybrid scaffold. Additionally, Saos-2 cells were used to assess cell viability and proliferation. Two types of control scaffolds were used (a 3D printed PCL frame and a hybrid scaffold without HA/β-TCP particles) for comparison, and the fabricated hybrid scaffold was verified to retain osteoconductive ceramic particles without losses. Moreover, the fabricated hybrid scaffold had high porosity and excellent microstructural interconnectivity. The in vitro Saos-2 cell experiments revealed superior cell proliferation and alkaline phosphatase assay results for the hybrid scaffold than the control scaffold. Hence, the proposed hybrid scaffold is a promising candidate for minimizing cost and duration of dental implant surgery.

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