scholarly journals Novel suction-based in vivo cutaneous DNA transfection platform

2021 ◽  
Vol 7 (45) ◽  
Author(s):  
Emran O. Lallow ◽  
Nandita C. Jhumur ◽  
Ijaz Ahmed ◽  
Sagar B. Kudchodkar ◽  
Christine C. Roberts ◽  
...  
Keyword(s):  
Dna Transfection

scholarly journals β–Amino Ester Polymers Facilitate in Vivo DNA Transfection and Adjuvant Plasmid DNA Immunization

2005 ◽  
Vol 12 (1) ◽  
pp. 164-170 ◽  
Author(s):  
John R. Greenland ◽  
Huining Liu ◽  
David Berry ◽  
Daniel G. Anderson ◽  
Woong-Ki Kim ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Immunization ◽  
Amino Ester ◽  
Dna Transfection

scholarly journals Definition of a Novel Plasmid-Based Gene Transfection Protocol of Mammalian Skeletal Muscles by Means of In Vivo Electroporation

2020 ◽  
Vol 21 (18) ◽  
pp. 6494
Author(s):  
Enrico P. Spugnini ◽  
Manuel Scimeca ◽  
Bruno Amadio ◽  
Giancarlo Cortese ◽  
Maurizio Fanciulli ◽  
...  
Keyword(s):  
Gene Transfection ◽  
Dna Transfection ◽  
In Vivo Electroporation ◽  
Electric Pulses ◽  
Hind Limbs ◽  
Transfection Protocol ◽  
Gait Abnormalities ◽  
Definition Of ◽  
The Right

We describe an original electroporation protocol for in vivo plasmid DNA transfection. The right hind limbs of C57 mice are exposed to a specifically designed train of permeabilizing electric pulses by transcutaneous application of tailored needle electrodes, immediately after the injection of pEGFP-C1 plasmid encoding GFP (Green Fluorescente Protein). The electroporated rodents show a greater GFP expression than the controls at three different time points (4, 10, and 15 days). The electroporated muscles display only mild interstitial myositis, with a significant increase in inflammatory cell infiltrates. Finally, mild gait abnormalities are registered in electroporated mice only in the first 48 h after the treatment. This protocol has proven to be highly efficient in terms of expression levels of the construct, is easy to apply since it does not require surgical exposure of the muscle and is well tolerated by the animals because it does not cause evident morphological and functional damage to the electroporated muscle.

scholarly journals P85, Optison microbubbles and ultrasound cooperate in mediating plasmid DNA transfection in mouse skeletal muscles in vivo

2011 ◽  
Vol 18 (2) ◽  
pp. 513-519 ◽  
Author(s):  
Yun-Chao Chen ◽  
Li-Ping Jiang ◽  
Na-Xiang Liu ◽  
Zhi-Hui Wang ◽  
Kai Hong ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Skeletal Muscles ◽  
Dna Transfection

scholarly journals Electroporation-Based Treatments in Urology

Cancers ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2208 ◽  
Author(s):  
Aleksander Kiełbik ◽  
Wojciech Szlasa ◽  
Jolanta Saczko ◽  
Julita Kulbacka
Keyword(s):  
Cell Membrane ◽  
Irreversible Electroporation ◽  
Underlying Mechanism ◽  
Extracellular Proteins ◽  
Dna Transfection ◽  
Membrane Phospholipids ◽  
Cancer Antigens ◽  
Urological Cancers

The observation that an application of a pulsed electric field (PEF) resulted in an increased permeability of the cell membrane has led to the discovery of the phenomenon called electroporation (EP). Depending on the parameters of the electric current and cell features, electroporation can be either reversible or irreversible. The irreversible electroporation (IRE) found its use in urology as a non-thermal ablative method of prostate and renal cancer. As its mechanism is based on the permeabilization of cell membrane phospholipids, IRE (as well as other treatments based on EP) provides selectivity sparing extracellular proteins and matrix. Reversible EP enables the transfer of genes, drugs, and small exogenous proteins. In clinical practice, reversible EP can locally increase the uptake of cytotoxic drugs such as cisplatin and bleomycin. This approach is known as electrochemotherapy (ECT). Few in vivo and in vitro trials of ECT have been performed on urological cancers. EP provides the possibility of transmission of genes across the cell membrane. As the protocols of gene electrotransfer (GET) over the last few years have improved, EP has become a well-known technique for non-viral cell transfection. GET involves DNA transfection directly to the cancer or the host skin and muscle tissue. Among urological cancers, the GET of several plasmids encoding prostate cancer antigens has been investigated in clinical trials. This review brings into discussion the underlying mechanism of EP and an overview of the latest progress and development perspectives of EP-based treatments in urology.

scholarly journals Ultrasound-targeted microbubble destruction enhances naked plasmid DNA transfection in rabbit Achilles tendons in vivo

Gene Therapy ◽  
2011 ◽  
Vol 19 (7) ◽  
pp. 703-710 ◽  
Author(s):  
L Qiu ◽  
L Zhang ◽  
L Wang ◽  
Y Jiang ◽  
Y Luo ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Transfection ◽  
Naked Plasmid ◽  
Naked Plasmid Dna

scholarly journals Femtosecond laser treatment enhances DNA transfection efficiency in vivo

2009 ◽  
Vol 16 (1) ◽  
pp. 36 ◽  
Author(s):  
Shaw-Wei D Tsen ◽  
Chao-Yi Wu ◽  
Avedis Meneshian ◽  
Sara I Pai ◽  
Chien-Fu Hung ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Laser Treatment ◽  
Transfection Efficiency ◽  
Dna Transfection

DNA-mediated transfection as a model for oncogenesis

1988 ◽  
Vol 66 (6) ◽  
pp. 594-616 ◽  
Author(s):  
Mary Pat Moyer ◽  
John W. Egan ◽  
J. Bradley Aust ◽  
Rex C. Moyer
Keyword(s):  
Animal Models ◽  
Human Cancer ◽  
Neoplastic Transformation ◽  
Dna Transfer ◽  
Dna Transfection ◽  
Tumor Induction ◽  
Oncogenic Potential ◽  
Cancer Initiation

DNA transfer technology has greatly contributed to progress in understanding molecular biology and genetics. In recent years, great efforts have been expended to determine the oncogenic potential of single, defined genes or complex gene mixtures as a prelude to defining the role those genes may play in neoplastic transformation in vitro and tumor induction in vivo. This paper reviews the currently available DNA transfection techniques and their application toward understanding cancer initiation and progression, and how the in vitro and animal models may apply to human cancer.

Structurally Flexible Triethanolamine Core PAMAM Dendrimers Are Effective Nanovectors for DNA Transfection in Vitro and in Vivo to the Mouse Thymus

2011 ◽  
Vol 22 (12) ◽  
pp. 2461-2473 ◽  
Author(s):  
Xiaoxuan Liu ◽  
Jiangyu Wu ◽  
Miriam Yammine ◽  
Jiehua Zhou ◽  
Paola Posocco ◽  
...  
Keyword(s):  
Pamam Dendrimers ◽  
Dna Transfection ◽  
Mouse Thymus

The Study on Poly-Lysine-Alginate Microcapsules Mediated Virus Genomic DNA Transfection

2013 ◽  
Vol 699 ◽  
pp. 298-301
Author(s):  
Guo Qing Wang ◽  
Dan Zhang ◽  
Liu Yuan Xia ◽  
Nai Sheng Zhang
Keyword(s):  
Biological Activity ◽  
Genomic Dna ◽  
Intramuscular Injection ◽  
Pseudorabies Virus ◽  
Dna Transfection ◽  
Viral Dna

The goal in this paper is to investigate the efficiency of poly-PLL (Poly-L-lysine)/ Alg (Alginate) vector mediated virus genomic DNA transfection and the virus genomic DNA’s biological activity in vivo. After Pseudorabies virus (PRV) genomic DNA being adhered to the porous СаСО3 particles, PLL and Alg were alternately polymerized on the surface of the porous DNA-СаСО3 particles to 7 layers, which were later dissolved them by EDTA to remove СаСО3 cores; the vectors in which the DNA were coated by poly-PLL/Alg, were harvested to infect the rabbits and observe the replication of viral DNA. Porous СаСО3 particles, which were obtained from the reaction between Na2CO3 and CaCl2, had an efficiency of absorbing DNA 1 µg/mg СаСО3 particles. After being coated by PLL/Alg, microcapsules were obtained with the diameter of 2-4 µm. 10.0 µg of poly-PLL/Alg-PRV DNA microcapsules could cause rabbits’ death by intramuscular injection. The identification of PCR shows that the death was caused by PRV infection. The results indicate that Poly-PLL/Alg microcapsules can mediate efficient transfection of DNA.

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