scholarly journals Longitudinal high-resolution imaging through a flexible intravital imaging window

2021 ◽  
Vol 7 (25) ◽  
pp. eabg7663
Author(s):  
Guillaume Jacquemin ◽  
Maria Benavente-Diaz ◽  
Samir Djaber ◽  
Aurélien Bore ◽  
Virginie Dangles-Marie ◽  
...  

Intravital microscopy (IVM) is a powerful technique that enables imaging of internal tissues at (sub)cellular resolutions in living animals. Here, we present a silicone-based imaging window consisting of a fully flexible, sutureless design that is ideally suited for long-term, longitudinal IVM of growing tissues and tumors. Crucially, we show that this window, without any customization, is suitable for numerous anatomical locations in mice using a rapid and standardized implantation procedure. This low-cost device represents a substantial technological and performance advance that facilitates intravital imaging in diverse contexts in higher organisms, opening previously unattainable avenues for in vivo imaging of soft and fragile tissues.

2021 ◽  
Author(s):  
Guillaume Jacquemin ◽  
Maria Benavente-Diaz ◽  
Samir Djaber ◽  
Aurélien Bore ◽  
Virginie Dangles-Marie ◽  
...  

AbstractIntravital microscopy (IVM) is a powerful technique that enables imaging of internal tissues at (sub)cellular resolutions in living animals. Here, we present a silicone-based imaging window consisting of a fully flexible, suture-less design that is ideally suited for long-term, longitudinal IVM of growing tissues and tumors. Crucially, we show that this window, without any customization, is suitable for numerous anatomical locations in mice using a rapid and standardized implantation procedure. This low-cost device represents a substantial technological and performance advance that facilitates intravital imaging in diverse contexts in higher organisms, opening new avenues for in vivo imaging of soft and fragile tissues.One-sentence summaryThis study presents a versatile, fully flexible imaging window that acts as an implantable transparent ‘second skin’ for small laboratory animal in vivo imaging.


2008 ◽  
Vol 2008 (2) ◽  
pp. pdb.prot4902-pdb.prot4902 ◽  
Author(s):  
B. E. Chen ◽  
J. T. Trachtenberg ◽  
A. J.G.D. Holtmaat ◽  
K. Svoboda

2009 ◽  
Vol 17 (6) ◽  
pp. 16-19 ◽  
Author(s):  
B. Cline ◽  
R. Luo ◽  
K. Kuhlmann

Many infectious diseases prevalent in the developing world, including malaria and tuberculosis, are difficult to diagnose on the basis of symptoms alone but can be accurately detected using microscope examination. Currently the expense, size, and fragility of optical microscopes impede their widespread use in resource-limited settings. Addressing these obstacles facing microscopy in the developing world is a pressing need; over 800,000 people, primarily children in Africa, die annually of malaria, and more than 1,500,000 people die annually of tuberculosis [1][2]. The aim of this study is to design and validate a microscope for use in the developing world that combines high-resolution imaging, extreme affordability, and long-term durability.


2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Woo Seok Kim ◽  
Sungcheol Hong ◽  
Milenka Gamero ◽  
Vivekanand Jeevakumar ◽  
Clay M. Smithhart ◽  
...  

AbstractThe vagus nerve supports diverse autonomic functions and behaviors important for health and survival. To understand how specific components of the vagus contribute to behaviors and long-term physiological effects, it is critical to modulate their activity with anatomical specificity in awake, freely behaving conditions using reliable methods. Here, we introduce an organ-specific scalable, multimodal, wireless optoelectronic device for precise and chronic optogenetic manipulations in vivo. When combined with an advanced, coil-antenna system and a multiplexing strategy for powering 8 individual homecages using a single RF transmitter, the proposed wireless telemetry enables low cost, high-throughput, and precise functional mapping of peripheral neural circuits, including long-term behavioral and physiological measurements. Deployment of these technologies reveals an unexpected role for stomach, non-stretch vagal sensory fibers in suppressing appetite and demonstrates the durability of the miniature wireless device inside harsh gastric conditions.


Author(s):  
Angelos A. Skodras ◽  
Jasmin K. Hefendehl ◽  
Jonas J. Neher

2021 ◽  
Author(s):  
Dag Børre Lillestøl ◽  
Odd Torbjørn Kårvand ◽  
Are Torstensen

Abstract This paper outlines an approach on how to improve the mooring integrity of existing long term mooring systems by using existing and commercially available data. It will be demonstrated how the use of AIS and hindcast weather data can be used to increase understanding of mooring systems and to monitor and quantify gaps between "as-designed", "as-installed" and "as-is" of a long term mooring system. Long term moored units have traditionally suffered from many early failures, caused by damages and errors introduced in the installation phase, and costly and unnecessary "late in life" failures. A fact rated high on the agenda of the underwriters. Numerous papers have been written on this topic, but it is only in recent years the industry have started to ensure that systems are inspected to a sufficient degree with respect to the physical condition, taking these learnings into account. However, the second important element, the calibration of the mooring analysis vs. actual vessel and mooring system behavior/performance, have not yet gotten the attention required. Deviations from the intended design are introduced in the installation phase of a mooring system. In addition, the design assumptions will never be fully accurate. The gap between the design assumptions and the actual system will increase over time, and the industry today do not focus on mapping and quantifying the effect of this gap sufficiently. The described method explains how one can introduce a pro-active approach, without installing onboard equipment, but rather utilizing algorithms on existing data and design documentation. This paper focuses on the use of AIS data in combination with historic weather/environmental data and seek to demonstrate how this low-cost method can provide useful information with respect to the mooring system. To emphasize the mapped importance of such calibrations, the July 2021 Edition of the in-service DNV Class Rules, DNVGL-OS-0300, formally introduces requirements to calibration of design assumptions of long term mooring units through use of survey data, service history and actual mooring system behavior in order to ensure a unit's mooring system condition and performance is known in light of the original design assumptions.


Nano Research ◽  
2020 ◽  
Vol 13 (8) ◽  
pp. 2239-2245 ◽  
Author(s):  
Xiulei Shi ◽  
Song Chen ◽  
Meng-Yao Luo ◽  
Biao Huang ◽  
Guozhen Zhang ◽  
...  

Nature ◽  
2002 ◽  
Vol 420 (6917) ◽  
pp. 788-794 ◽  
Author(s):  
Joshua T. Trachtenberg ◽  
Brian E. Chen ◽  
Graham W. Knott ◽  
Guoping Feng ◽  
Joshua R. Sanes ◽  
...  

2019 ◽  
Vol 2 (5) ◽  
pp. 327-335 ◽  
Author(s):  
Tasneem Z. Khatib ◽  
Paul A.R. Meyer ◽  
Jed Lusthaus ◽  
Ilya Manyakin ◽  
Yusuf Mushtaq ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document