scholarly journals Breakthrough in purification of fossil pollen for dating of sediments by a new large-particle on-chip sorter

2021 ◽  
Vol 7 (16) ◽  
pp. eabe7327
Author(s):  
Y. Kasai ◽  
C. Leipe ◽  
M. Saito ◽  
H. Kitagawa ◽  
S. Lauterbach ◽  
...  
Keyword(s):  
High Throughput ◽  
Large Particle ◽  
Biological Research ◽  
Fossil Pollen ◽  
Maximum Dimension ◽  
Radiocarbon Dates ◽  
Large Size ◽  
Particle Sorting ◽  
Activation System ◽  
On Chip

Particle sorting is a fundamental method in various fields of medical and biological research. However, existing sorting applications are not capable for high-throughput sorting of large-size (>100 micrometers) particles. Here, we present a novel on-chip sorting method using traveling vortices generated by on-demand microjet flows, which locally exceed laminar flow condition, allowing for high-throughput sorting (5 kilohertz) with a record-wide sorting area of 520 micrometers. Using an activation system based on fluorescence detection, the method successfully sorted 160-micrometer microbeads and purified fossil pollen (maximum dimension around 170 micrometers) from lake sediments. Radiocarbon dates of sorting-derived fossil pollen concentrates proved accurate, demonstrating the method’s ability to enhance building chronologies for paleoenvironmental records from sedimentary archives. The method is capable to cover urgent needs for high-throughput large-particle sorting in genomics, metabolomics, and regenerative medicine and opens up new opportunities for the use of pollen and other microfossils in geochronology, paleoecology, and paleoclimatology.

Palaeolithic Big Game Hunting at HP766 in Wadi Umm Rahau, Northern Sudan

2012 ◽  
Vol 10 (2) ◽  
pp. 165-174 ◽  
Author(s):  
Achilles Gautier ◽  
Daniel Makowiecki ◽  
Henryk Paner ◽  
Wim Van Neer
Keyword(s):  
Late Prehistoric ◽  
Large Mammals ◽  
Radiocarbon Dates ◽  
Large Size ◽  
Animal Bone ◽  
Game Hunting ◽  
Big Game Hunting ◽  
Bone Remains

HP766, discovered by the Gdansk Archaeological Museum Expedition (GAME) in the region immediately upstream the Merowe Dam in North Sudan and now under water, is one of the few palaeolithic sites with animal bone remains in the country. The archaeological deposits, the large size of the site, the lithics and the radiocarbon dates indicate occupation of a silt terrace of the Nile in late MSA and perhaps LSA times. Large and very large mammals predominate markedly among the recovered bone remains and it would seem that the palaeolithic hunters focused on such game. They could corner these animals on the site which is partially surrounded by high bedrock outcrops. Moreover swampy conditions of the site after the retreat of the annual Nile flood may have rendered less mobile the prey animals. According to this scenario, HP766 would testify to the ecological skills and generational memory of late prehistoric man in Sudan.

Next-Generation Sequencing: An Emerging Tool for Drug Designing

2019 ◽  
Vol 25 (31) ◽  
pp. 3350-3357 ◽  
Author(s):  
Pooja Tripathi ◽  
Jyotsna Singh ◽  
Jonathan A. Lal ◽  
Vijay Tripathi
Keyword(s):  
Next Generation Sequencing ◽  
High Throughput ◽  
Large Scale ◽  
Massively Parallel Sequencing ◽  
Genomic Research ◽  
Biological Research ◽  
Next Generation ◽  
Human Welfare ◽  
Drug Designing ◽  
Generation Sequencing

Background: With the outbreak of high throughput next-generation sequencing (NGS), the biological research of drug discovery has been directed towards the oncology and infectious disease therapeutic areas, with extensive use in biopharmaceutical development and vaccine production. Method: In this review, an effort was made to address the basic background of NGS technologies, potential applications of NGS in drug designing. Our purpose is also to provide a brief introduction of various Nextgeneration sequencing techniques. Discussions: The high-throughput methods execute Large-scale Unbiased Sequencing (LUS) which comprises of Massively Parallel Sequencing (MPS) or NGS technologies. The Next geneinvolved necessarily executes Largescale Unbiased Sequencing (LUS) which comprises of MPS or NGS technologies. These are related terms that describe a DNA sequencing technology which has revolutionized genomic research. Using NGS, an entire human genome can be sequenced within a single day. Conclusion: Analysis of NGS data unravels important clues in the quest for the treatment of various lifethreatening diseases and other related scientific problems related to human welfare.

High-throughput concentration of rare malignant tumor cells from large-volume effusions by multistage inertial microfluidics

Lab on a Chip ◽  
2022 ◽  
Author(s):  
Nan Xiang ◽  
Zhonghua Ni
Keyword(s):  
Tumor Cells ◽  
High Throughput ◽  
Malignant Tumor ◽  
Large Volume ◽  
Concentration Factor ◽  
Pleural Effusions ◽  
Inertial Microfluidics ◽  
Low Concentration ◽  
Malignant Pleural Effusions ◽  
On Chip

On-chip concentration of rare malignant tumor cells (MTCs) in malignant pleural effusions (MPEs) with a large volume is challenging. Previous microfluidic concentrators suffer from a low concentration factor (CF) and...

scholarly journals High-throughput screening of large volumes of whole blood using structured illumination and fluorescent on-chip imaging

Lab on a Chip ◽  
2012 ◽  
Vol 12 (23) ◽  
pp. 4968 ◽  
Author(s):  
Serap Altay Arpali ◽  
Caglar Arpali ◽  
Ahmet F. Coskun ◽  
Hsin-Hao Chiang ◽  
Aydogan Ozcan
Keyword(s):  
High Throughput ◽  
Whole Blood ◽  
High Throughput Screening ◽  
Structured Illumination ◽  
On Chip

scholarly journals Large tare vessels of the bronze age Turganic settlement in Orenburg Region

2016 ◽  
Vol 5 (4) ◽  
pp. 91-98 ◽  
Author(s):  
Natalya Petrovna Salugina ◽  
Nina Leonidovna Morgunova ◽  
Mihail Aleksandrovich Turetskii
Keyword(s):  
Bronze Age ◽  
Burial Mound ◽  
North Caucasus ◽  
Radiocarbon Dates ◽  
Orenburg Region ◽  
The North ◽  
Type Selection ◽  
Large Size ◽  
Culture Formation ◽  
Middle Bronze Age

In the ceramic collection of Turganic settlement in the Orenburg region there is a group of bronze age pottery, which by its morphological and technological indicators stands out sharply from the main group of dishes. They are large size vessels with massive aureoles and distended body. The authors called these vessels hums. The aim of this study is to identify cultural-chronological position of the specified group of dishes in the system of the antiquities of the early - middle bronze age. Within this group the authors distinguish two types. The basis for type selection was the particular design of the upper part of the vessel. The first type is ceramics from Turganic settlement and the vessel from the burial mound Perevolotsky I. Morphological and technological features, and a series of radiocarbon dates has allowed to date these vessels to the time of the yamnaya culture formation in the Volga-Ural region (Repinsky stage). The authors suggest that the appearance of such vessels should be an imitation of the Maikop pottery. It could be penetration of small groups of craftsmen or the intensification of contacts with the population of the North Caucasus. The second type of pottery from Turganic settlement is similar to the burial mound Kardailovsky I (mound 1, burial 3) in Orenburg region, in the Northern pre-Caspian, region of the Samara river, Kuban and the Dnieper. Researchers have noted the scarcity and originality of this dish. The chronological and cultural position of such vessels is determined within the III Millennium BC (calibrated values).

scholarly journals Bioinformatics in otolaryngology research. Part one: concepts in DNA sequencing and gene expression analysis

2014 ◽  
Vol 128 (10) ◽  
pp. 848-858 ◽  
Author(s):  
T J Ow ◽  
K Upadhyay ◽  
T J Belbin ◽  
M B Prystowsky ◽  
H Ostrer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Data Storage ◽  
Expression Analysis ◽  
High Throughput ◽  
Gene Expression Analysis ◽  
Biological Data ◽  
Biological Research ◽  
Nucleotide Sequencing ◽  
New Era ◽  
Crucial Component

AbstractBackground:Advances in high-throughput molecular biology, genomics and epigenetics, coupled with exponential increases in computing power and data storage, have led to a new era in biological research and information. Bioinformatics, the discipline devoted to storing, analysing and interpreting large volumes of biological data, has become a crucial component of modern biomedical research. Research in otolaryngology has evolved along with these advances.Objectives:This review highlights several modern high-throughput research methods, and focuses on the bioinformatics principles necessary to carry out such studies. Several examples from recent literature pertinent to otolaryngology are provided. The review is divided into two parts; this first part discusses the bioinformatics approaches applied in nucleotide sequencing and gene expression analysis.Conclusion:This paper demonstrates how high-throughput nucleotide sequencing and transcriptomics are changing biology and medicine, and describes how these changes are affecting otorhinolaryngology. Sound bioinformatics approaches are required to obtain useful information from the vast new sources of data.

scholarly journals Calcinated gold nanoparticle arrays for on-chip, multiplexed and matrix-free mass spectrometric analysis of peptides and small molecules

Nanoscale ◽  
2016 ◽  
Vol 8 (3) ◽  
pp. 1665-1675 ◽  
Author(s):  
Samuel S. Hinman ◽  
Chih-Yuan Chen ◽  
Jicheng Duan ◽  
Quan Cheng
Keyword(s):  
Small Molecules ◽  
Gold Nanoparticle ◽  
High Throughput ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Nanoparticle Arrays ◽  
Silicate Coating ◽  
On Chip ◽  
Matrix Free

Patterned gold nanoparticle microarrays, with a nanoscale silicate coating, have been developed for on-chip, high-throughput mass spectrometric analysis of biomolecules.

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