scholarly journals Expanding and optimizing 3D bioprinting capabilities using complementary network bioinks

2020 ◽  
Vol 6 (38) ◽  
pp. eabc5529 ◽  
Author(s):  
Liliang Ouyang ◽  
James P. K. Armstrong ◽  
Yiyang Lin ◽  
Jonathan P. Wojciechowski ◽  
Charlotte Lee-Reeves ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Synthetic Polymers ◽  
3D Bioprinting ◽  
Poly Ethylene Glycol ◽  
Encapsulated Cells ◽  
Heterogeneous Structures ◽  
Poly Ethylene

A major challenge in three-dimensional (3D) bioprinting is the limited number of bioinks that fulfill the physicochemical requirements of printing while also providing a desirable environment for encapsulated cells. Here, we address this limitation by temporarily stabilizing bioinks with a complementary thermo-reversible gelatin network. This strategy enables the effective printing of biomaterials that would typically not meet printing requirements, with instrument parameters and structural output largely independent of the base biomaterial. This approach is demonstrated across a library of photocrosslinkable bioinks derived from natural and synthetic polymers, including gelatin, hyaluronic acid, chondroitin sulfate, dextran, alginate, chitosan, heparin, and poly(ethylene glycol). A range of complex and heterogeneous structures are printed, including soft hydrogel constructs supporting the 3D culture of astrocytes. This highly generalizable methodology expands the palette of available bioinks, allowing the biofabrication of constructs optimized to meet the biological requirements of cell culture and tissue engineering.

scholarly journals Three-Dimensional Microassembly of Cell-Laden Microplates by in situ Gluing with Photocurable Hydrogels

2014 ◽  
Vol 8 (1) ◽  
pp. 95-101 ◽  
Author(s):  
Shotaro Yoshida ◽  
◽  
Koji Sato ◽  
Shoji Takeuchi
Keyword(s):  
Tissue Engineering ◽  
Ethylene Glycol ◽  
Interaction Analysis ◽  
Three Dimensional ◽  
Cell Interaction ◽  
Poly Ethylene Glycol ◽  
Peg Hydrogels ◽  
Assembly Method ◽  
Poly Ethylene

This paper describes a method for assembling cellladen microplates into three-dimensional (3D) microstructures by in situ gluing using photocurable hydrogels. We picked up cell-laden microplates with microtweezers, placed the plate perpendicular to one another on a microgroove device, and glued them by local photopolymerization of biocompatible Poly (Ethylene Glycol) (PEG) hydrogels. The advantage of this assembly method is its ability to construct 3D biological microstructures with targeted cells. We demonstrated the assembly of a 3D half-cube microstructure with genetically labeled cell-laden microplates. We believe our method is useful for engineering the positions of cells in 3D configurations for cell-cell interaction analysis and tissue engineering.

The Mechanical Behavior of Engineered Hydrogels

2009 ◽  
Author(s):  
Audrey L. Earnshaw ◽  
Justine J. Roberts ◽  
Garret D. Nicodemus ◽  
Stephanie J. Bryant ◽  
Virginia L. Ferguson
Keyword(s):  
Tissue Engineering ◽  
Ethylene Glycol ◽  
Mechanical Behavior ◽  
Three Dimensional ◽  
Engineering Application ◽  
Tissue Engineering Application ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
A Chain

Agarose and poly(ethylene-glycol) (PEG) are commonly used as scaffolds for cell and tissue engineering applications [1]. Agarose is a natural biomaterial that is thought to be inert [2] and permits growing cells and tissues in a three-dimensional suspension [3]. Gels synthesized from photoreactive poly(ethylene glycol) (PEG) macromonomers are well suited as cell carriers because they can be rapidly photopolymerized in vivo by a chain radical polymerization that is not toxic to cells, including chondrocytes. This paper explores the differences in mechanical behavior between agarose, a physically cross-linked hydrogel, and PEG, a chemically cross-linked hydrogel, to set the foundation for choosing hydrogel properties and chemistries for a desired tissue engineering application.

Projection Microfabrication of Three-Dimensional Scaffolds for Tissue Engineering

2008 ◽  
Vol 130 (2) ◽  
Author(s):  
Li-Hsin Han ◽  
Gazell Mapili ◽  
Shaochen Chen ◽  
Krishnendu Roy
Keyword(s):  
Tissue Engineering ◽  
Uv Light ◽  
Three Dimensional ◽  
Layer By Layer ◽  
Poly Ethylene Glycol ◽  
Glycol Diacrylate ◽  
Cellular Attachment ◽  
Poly Ethylene ◽  
Feature Resolution ◽  
Graphic Software

This article presents a micromanufacturing method for direct projection printing of three-dimensional scaffolds for applications in the field of tissue engineering by using a digital micromirror-array device (DMD) in a layer-by-layer process. Multilayered scaffolds are microfabricated using curable materials through an ultraviolet (UV) photopolymerization process. The prepatterned UV light is projected onto the photocurable polymer solution by creating the “photomask” design with a graphic software. Poly(ethylene glycol) diacrylate is mixed with a small amount of dye (0.3wt%) to enhance the fabrication resolution of the scaffold. The DMD fabrication system is equipped with a purging mechanism to prevent the accumulation of oligomer, which could interfere with the feature resolution of previously polymerized layers. The surfaces of the predesigned multilayered scaffold are covalently conjugated with fibronectin for efficient cellular attachment. Our results show that murine marrow-derived progenitor cells successfully attached to fibronectin-modified scaffolds.

3D bioprinting of oligo(poly[ethylene glycol] fumarate) for bone and nerve tissue engineering

2020 ◽  
Vol 109 (1) ◽  
pp. 6-17
Author(s):  
Xifeng Liu ◽  
Bipin Gaihre ◽  
Matthew N. George ◽  
A. Lee Miller ◽  
Haocheng Xu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Ethylene Glycol ◽  
Nerve Tissue ◽  
3D Bioprinting ◽  
Poly Ethylene Glycol ◽  
Nerve Tissue Engineering ◽  
Poly Ethylene

Engineering a Three-Dimensional In Vitro Drug Testing Platform for Glioblastoma

2015 ◽  
Vol 6 (4) ◽  
Author(s):  
Metin Akay ◽  
Duong T. Nguyen ◽  
Yantao Fan ◽  
Yasemin M. Akay
Keyword(s):  
Cell Culture ◽  
Drug Testing ◽  
Three Dimensional ◽  
In Vitro Tests ◽  
Valuable Insight ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Cancer Spheroids

Three-dimensional (3D) in vivo cell culture modeling is quickly emerging as a platform to replace two-dimensional (2D) monolayer cell culture in vitro tests. Three-dimensional tumor models mimic physiological conditions and provide valuable insight of the tumor cell response to drug discovery application. In this study, we used poly(ethylene glycol) (PEG) hydrogel microwells to generate 3D brain cancer spheroids and studied their treatment with anticancer drugs in single or combination treatment. Glioblastoma (GBM) spheroids were grown through 14 days before infecting with two drugs: Pitavastatin and Irinotecan at various concentrations. A significant cell lysis was observed and cell viability decreased to lower than 7% when drugs were combined at the concentration Pitavastatin 10 μM and Irinotecan 50 μM to infect after 7 days. These findings demonstrate a promising platform—PEG hydrogel microwells—that should be an efficient way to test the drug sensitivity in vitro as well as application in different studies.

scholarly journals Collagen as Bioink for Bioprinting: A Comprehensive Review.

2020 ◽  
Vol 6 (3) ◽  
Author(s):  
Egor Olegovich Osidak ◽  
Vadim Igorevich Kozhukhov ◽  
Mariya Sergeevna Osidak ◽  
Sergey Petrovich Domogatskiy
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Three Dimensional ◽  
Additive Technologies ◽  
Important Task ◽  
Morphological Properties ◽  
3D Bioprinting ◽  
Comprehensive Review ◽  
Pure Collagen

Biomaterials made using collagen are successfully used as a three-dimensional (3D) substrate for cell culture and considered to be promising scaffolds for creating artificial tissues. An important task that arises for engineering such materials is the simulation of physical and morphological properties of tissues, which must be restored or replaced. Modern additive technologies, including 3D bioprinting, can be applied to successfully solve this task. This review provides the latest evidence on advances of 3D bioprinting with collagen in the field of tissue engineering. It contains modern approaches for printing pure collagen bioinks consisting only of collagen and cells, as well as the obtained results from the use of pure collagen bioinks in different fields of tissue engineering.

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