scholarly journals Meiosis occurs normally in the fetal ovary of mice lacking all retinoic acid receptors

2020 ◽  
Vol 6 (21) ◽  
pp. eaaz1139
Author(s):  
Nadège Vernet ◽  
Diana Condrea ◽  
Chloé Mayere ◽  
Betty Féret ◽  
Muriel Klopfenstein ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Nuclear Receptors ◽  
Current Model ◽  
Retinoic Acid Receptors ◽  
Null Alleles ◽  
Fetal Life ◽  
Adult Recipient ◽  
Molecular Signal ◽  
Fetal Ovary ◽  
Meiotic Initiation

Gametes are generated through a specialized cell differentiation process, meiosis, which, in ovaries of most mammals, is initiated during fetal life. All-trans retinoic acid (ATRA) is considered as the molecular signal triggering meiosis initiation. In the present study, we analyzed female fetuses ubiquitously lacking all ATRA nuclear receptors (RAR), obtained through a tamoxifen-inducible cre recombinase-mediated gene targeting approach. Unexpectedly, mutant oocytes robustly expressed meiotic genes, including the meiotic gatekeeper STRA8. In addition, ovaries from mutant fetuses grafted into adult recipient females yielded offspring bearing null alleles for all Rar genes. Thus, our results show that RAR are fully dispensable for meiotic initiation, as well as for the production of functional oocytes. Assuming that the effects of ATRA all rely on RAR, our study goes against the current model according to which meiosis is triggered by endogenous ATRA in the developing ovary. It therefore revives the search for the meiosis-inducing substance.

scholarly journals Meiosis Initiates In The Fetal Ovary Of Mice Lacking All Retinoic Acid Receptor Isotypes

2019 ◽  
Author(s):  
Nadège Vernet ◽  
Manuel Mark ◽  
Diana Condrea ◽  
Betty Féret ◽  
Muriel Klopfenstein ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Germ Cells ◽  
Congenital Abnormalities ◽  
Retinoic Acid Receptor ◽  
Current Model ◽  
Fetal Life ◽  
Genetic Approach ◽  
Molecular Signal ◽  
Fetal Ovary ◽  
Multiple Congenital Abnormalities

AbstractGametes are generated through a specialized cell differentiation process, meiosis which, in most mammals, is initiated in ovaries during fetal life. It is widely admitted that all-trans retinoic acid (ATRA) is the molecular signal triggering meiosis initiation in mouse female germ cells, but a genetic approach in which ATRA synthesis is impaired disputes this proposal. In the present study, we investigated the contribution of endogenous ATRA to meiosis by analyzing fetuses lacking all RARs ubiquitously, obtained through a tamoxifen-inducible cre recombinase-mediated gene targeting approach. Efficient ablation of RAR-coding genes was assessed by the multiple congenital abnormalities displayed by the mutant fetuses. Unexpectedly, their germ cells robustly expressed STRA8, REC8, SYCP1 and SYCP3, showing that RAR are actually dispensable up to the zygotene stage of meiotic prophase I. Thus our study goes against the current model according to which meiosis is triggered by endogenous ATRA in the developing ovary and revives the identification of the meiosis-preventing substance synthesized by CYP26B1 in the fetal testis.

scholarly journals SMRTε, a corepressor variant, interacts with a restricted subset of nuclear receptors, including the retinoic acid receptors α and β

2012 ◽  
Vol 351 (2) ◽  
pp. 306-316 ◽  
Author(s):  
Brenda J. Mengeling ◽  
Michael L. Goodson ◽  
William Bourguet ◽  
Martin L. Privalsky
Keyword(s):  
Retinoic Acid ◽  
Nuclear Receptors ◽  
Retinoic Acid Receptors

Retinoic acid receptors and cellular retinoid binding proteins. III. Their differential transcript distribution during mouse nervous system development

Development ◽  
1993 ◽  
Vol 118 (1) ◽  
pp. 267-282 ◽  
Author(s):  
E. Ruberte ◽  
V. Friederich ◽  
P. Chambon ◽  
G. Morriss-Kay
Keyword(s):  
Nervous System ◽  
Retinoic Acid ◽  
Nuclear Receptors ◽  
Binding Proteins ◽  
Binding Protein ◽  
Retinoic Acid Receptors ◽  
Type I ◽  
Crabp I ◽  
Retinoid Binding Proteins ◽  
Crabp Ii

We have studied the transcript distribution of the retinoic acid receptors (RARs) and the cytoplasmic retinoid binding proteins during embryonic development of the mouse nervous system. Of the three retinoic acid receptors, only RAR-gamma was not expressed in developing neural structures. RAR-beta and RAR-alpha both showed rostral limits of expression in the medulla oblongata equivalent to their patterns of expression in the neuroepithelium of the early hindbrain neural tube. Within their expression domains in the spinal cord and brain, RAR-alpha was ubiquitously expressed, whereas RAR-beta transcripts showed very specific patterns of expression, suggesting that this receptor is involved in mediating retinoic acid-induced gene expression in relation to the development of specific neural structures or pathways. The cytoplasmic binding proteins, cellular retinoic acid binding proteins type I and II (CRABP I and CRABP II) and cellular retinol binding protein type I (CRBP I), were widely distributed in developing neural structures. Their differential spatiotemporal patterns of expression suggest that fine regional control of availability of retinoic acid (RA) to the nuclear receptors plays an important role in organization and differentiation of the nervous system. For instance, expression of CRABP I in the migrating cells that give rise to the olivary and pontine nuclei, which develop abnormally in conditions of retinoid excess, is consistent with observations from a variety of other systems indicating that CRABP I limits the access of RA to the nuclear receptors in normal physiological conditions. Similarly, expression of CRBP I in the choroid plexuses, which develop abnormally in conditions of vitamin A deficiency, is consistent with observations indicating that this binding protein mediates the synthesis of RA in tissues requiring high levels of RA for their normal developmental programme. RAR-beta and CRABP II, which are both RA-inducible, were coexpressed with CRBP I in the choroid plexus and in many other sites, perhaps reflecting the fact that all three genes are RA-inducible. The function of CRABP II is not well understood; its domains of expression showed overlaps with both CRABP I and CRBP I.

Expression of nuclear retinoic acid receptors during mouse odontogenesis

1994 ◽  
Vol 57 (3) ◽  
pp. 195-203 ◽  
Author(s):  
Agnès Bloch-Zupan ◽  
Didier Décimo ◽  
Maria Loriot ◽  
Manuel P. Mark ◽  
Jean Victor Ruch
Keyword(s):  
Retinoic Acid ◽  
Retinoic Acid Receptors

scholarly journals Multiple retinoid-responsive receptors in a single cell: families of retinoid "X" receptors and retinoic acid receptors in the Xenopus egg.

1992 ◽  
Vol 89 (6) ◽  
pp. 2321-2325 ◽  
Author(s):  
B. Blumberg ◽  
D. J. Mangelsdorf ◽  
J. A. Dyck ◽  
D. A. Bittner ◽  
R. M. Evans ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Single Cell ◽  
Retinoic Acid Receptors ◽  
Retinoid X Receptors ◽  
Xenopus Egg ◽  
X Receptors
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