scholarly journals Super-resolution nanoscopy by coherent control on nanoparticle emission

2020 ◽  
Vol 6 (16) ◽  
pp. eaaw6579
Author(s):  
Congyue Liu ◽  
Wei Liu ◽  
Shufeng Wang ◽  
Hongjia Li ◽  
Zhilong Lv ◽  
...  
Keyword(s):  
Coherent Control ◽  
High Efficiency ◽  
Super Resolution ◽  
General Strategy ◽  
Wide Field ◽  
Multiphoton Imaging ◽  
Two Photon ◽  
Characteristic Emission ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Super-resolution nanoscopy based on wide-field microscopic imaging provided high efficiency but limited resolution. Here, we demonstrate a general strategy to push its resolution down to ~50 nm, which is close to the range of single molecular localization microscopy, without sacrificing the wide-field imaging advantage. It is done by actively and simultaneously modulating the characteristic emission of each individual emitter at high density. This method is based on the principle of excited state coherent control on single-particle two-photon fluorescence. In addition, the modulation efficiently suppresses the noise for imaging. The capability of the method is verified both in simulation and in experiments on ZnCdS quantum dot–labeled films and COS7 cells. The principle of coherent control is generally applicable to single-multiphoton imaging and various probes.

scholarly journals Use of coherent control for selective two-photon fluorescence microscopy in live organisms

2006 ◽  
Vol 14 (2) ◽  
pp. 759 ◽  
Author(s):  
Jennifer P. Ogilvie ◽  
Delphine Débarre ◽  
Xavier Solinas ◽  
Jean-Louis Martin ◽  
Emmanuel Beaurepaire ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Coherent Control ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Optimizing the Two Photon Fluorescence of Coumarin 151 Using Coherent Control Technique

2011 ◽  
Vol 31 (s1) ◽  
pp. s100206
Author(s):  
褚赛赛 Chu Saisai ◽  
王树峰 Wang Shufeng ◽  
邓勇开 Deng Yongkai ◽  
杨宏 Yang Hong ◽  
龚旗煌 Gong Qihuang
Keyword(s):  
Coherent Control ◽  
Control Technique ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Hybrid quantum dot - collagen extracellular matrices for in situ optical monitoring of cardiomyocyte activity by two-photon fluorescence

2020 ◽  
Author(s):  
Stijn Jooken ◽  
Yovan de Coene ◽  
Olivier Deschaume ◽  
Olga Krylychkina ◽  
Thierry Verbiest ◽  
...  
Keyword(s):  
Cardiac Tissue ◽  
Tissue Growth ◽  
Inorganic Nanoparticles ◽  
Extracellular Matrices ◽  
Cellular Functions ◽  
Multiphoton Imaging ◽  
Two Photon ◽  
Current Clamp Mode ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

ABSTRACTThe incorporation of functional nanoparticles in scaffolds for tissue constructs has led to the creation of artificial extracellular matrices that more accurately mimic the cues present in the native microenvironment of developing tissue. Additionally, light-sensitive inorganic nanoparticles can act as cell biosensors and report on the physiological parameters during tissue growth and organization. In this work, we functionalized collagen nanofibers with semiconductor quantum dots (QDs) and thereby created artificial extracellular matrices that can optically report on cardiomyocyte activity based on QD two-photon fluorescence. We have applied these optically-addressable nanofiber matrices to monitor activities of primary cardiomyocytes and compared the optical responses with patch-clamp data. Combining the long-term stability of QD fluorescence with the deeper light penetration depths achievable through multiphoton imaging, this approach can be used for continuous monitoring of cellular functions in cardiac tissue engineering.Abstract FigureConcept illustration: optical readout of cardiomyocyte activity with QD-functionalized collagen networks. Whole-cell current-clamp mode is used here to simultaneously monitor changes in the transmembrane voltage while the QD two-photon fluorescence is recorded.

scholarly journals Wide-field multiphoton imaging through scattering media without correction

2018 ◽  
Vol 4 (10) ◽  
pp. eaau1338 ◽  
Author(s):  
Adrià Escobet-Montalbán ◽  
Roman Spesyvtsev ◽  
Mingzhou Chen ◽  
Wardiya Afshar Saber ◽  
Melissa Andrews ◽  
...  
Keyword(s):  
A Priori ◽  
Super Resolution ◽  
Mean Free Path ◽  
Wide Field ◽  
Biomedical Sciences ◽  
Scattering Media ◽  
Multiphoton Imaging ◽  
Two Photon ◽  
Temporal Focusing ◽  
Path Lengths

Optical approaches to fluorescent, spectroscopic, and morphological imaging have made exceptional advances in the last decade. Super-resolution imaging and wide-field multiphoton imaging are now underpinning major advances across the biomedical sciences. While the advances have been startling, the key unmet challenge to date in all forms of optical imaging is to penetrate deeper. A number of schemes implement aberration correction or the use of complex photonics to address this need. In contrast, we approach this challenge by implementing a scheme that requires no a priori information about the medium nor its properties. Exploiting temporal focusing and single-pixel detection in our innovative scheme, we obtain wide-field two-photon images through various turbid media including a scattering phantom and tissue reaching a depth of up to seven scattering mean free path lengths. Our results show that it competes favorably with standard point-scanning two-photon imaging, with up to a fivefold improvement in signal-to-background ratio while showing significantly lower photobleaching.

Increasing two-photon fluorescence signals by coherent control

2006 ◽  
Author(s):  
Eric R. Tkaczyk ◽  
Augustin Mignot ◽  
Jing Yong Ye ◽  
Istvan Majoros ◽  
James R. Baker, Jr. ◽  
...  
Keyword(s):  
Coherent Control ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Lysosomal targeted NIR photosensitizer for photodynamic therapy and two-photon fluorescent imaging

2021 ◽  
Author(s):  
Ruiyuan Liu ◽  
Yuping Zhou ◽  
Di Zhang ◽  
Genghan He ◽  
Chuang Liu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Fluorescent Imaging ◽  
High Fidelity ◽  
Design And Synthesis ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Design and synthesis of near-infrared (NIR) emissive fluorophore for imaging of organelle and photodynamic therapy has received enormous attention. Hence, NIR emissive fluorophore of high-fidelity lysosome targeting, two-photon fluorescence imaging,...

scholarly journals Molecular design strategy to alleviate environmental interference on two-photon fluorescence probes

2021 ◽  
Vol 2 (6) ◽  
pp. 100471
Author(s):  
Xingxing Zhang ◽  
Tianbing Ren ◽  
Qianling Zhang ◽  
Ting Zeng ◽  
Zhe Li ◽  
...  
Keyword(s):  
Molecular Design ◽  
Design Strategy ◽  
Fluorescence Probes ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence ◽  
Environmental Interference
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