scholarly journals Does the U.S. public support using gene drives in agriculture? And what do they want to know?

2019 ◽  
Vol 5 (9) ◽  
pp. eaau8462 ◽  
Author(s):  
Michael S. Jones ◽  
Jason A. Delborne ◽  
Johanna Elsensohn ◽  
Paul D. Mitchell ◽  
Zachary S. Brown
Keyword(s):  
Department Of Defense ◽  
Native Species ◽  
Public Support ◽  
Responsible Innovation ◽  
Gene Drive ◽  
Target Species ◽  
Department Of Agriculture ◽  
Potential Risks ◽  
The One ◽  
Gene Drives

Gene drive development is progressing more rapidly than our understanding of public values toward these technologies. We analyze a statistically representative survey (n = 1018) of U.S. adult attitudes toward agricultural gene drives. When informed about potential risks, benefits, and two previously researched applications, respondents’ support/opposition depends heavily (+22%/−19%) on whether spread of drives can be limited, non-native versus native species are targeted (+12%/−9%), or the drive replaces versus suppresses target species (±2%). The one-fifth of respondents seeking out non–GMO–labeled food are more likely to oppose drives, although their support exceeds opposition for limited applications. Over 62% trust U.S. universities and the Department of Agriculture to research gene drives, with the private sector and Department of Defense viewed as more untrustworthy. Uncertain human health and ecological effects are the public’s most important concerns to resolve. These findings can inform responsible innovation in gene drive development and risk assessment.

scholarly journals Invasion and migration of spatially self-limiting gene drives: a comparative analysis

2017 ◽  
Author(s):  
Sumit Dhole ◽  
Michael R. Vella ◽  
Alun L. Loyd ◽  
Fred Gould
Keyword(s):  
Target Population ◽  
Gene Drive ◽  
Fitness Costs ◽  
Invasion And Migration ◽  
Migration Rates ◽  
Chain System ◽  
Drive Systems ◽  
And Migration ◽  
The One ◽  
Gene Drives

AbstractRecent advances in research on gene drives have produced genetic constructs that could theoretically spread a desired gene (payload) into all populations of a species, with a single release in one place. This attribute has advantages, but also comes with risks and ethical concerns. There has been a call for research on gene drive systems that are spatially and/or temporally self-limiting. Here we use a population genetics model to compare the expected characteristics of three spatially self-limiting gene drive systems: one-locus underdominance, two-locus underdominance, and daisy-chain drives. We find large differences between these gene drives in the minimum release size required for successfully driving a payload into a population. The daisy-chain system is the most efficient, requiring the smallest release, followed by the two-locus underdominance system, and then the one-locus underdominance system. However, when the target population exchanges migrants with a non-target population, the gene drives requiring smaller releases suffer from higher risks of unintended spread. For payloads that incur relatively low fitness costs (up to 30%), a simple daisy-chain drive is practically incapable of remaining localized, even with migration rates as low as 0.5% per generation. The two-locus underdominance system can achieve localized spread under a broader range of migration rates and of payload fitness costs, while the one-locus underdominance system largely remains localized. We also find differences in the extent of population alteration and in the permanence of the alteration achieved by the three gene drives. The two-locus underdominance system does not always spread the payload to fixation, even after successful drive, while the daisy-chain system can, for a small set of parameter values, achieve a temporally-limited spread of the payload. These differences could affect the suitability of each gene drive for specific applications.Note:This manuscript has been accepted for publication in the journal Evolutionary Applications and is pending publication. We suggest that any reference to or quotation of this article should be made with this recognition.

scholarly journals Modeling homing suppression gene drive in haplodiploid organisms

2021 ◽  
Author(s):  
Yiran Liu ◽  
Jackson Champer
Keyword(s):  
Great Promise ◽  
Resistance Allele ◽  
Gene Drive ◽  
Wild Type ◽  
Target Species ◽  
Strong Suppression ◽  
Continuous Space ◽  
Cleavage Activity ◽  
Fertility Gene ◽  
Gene Drives

Gene drives have shown great promise for suppression of pest populations. These engineered alleles can function by a variety of mechanisms, but the most common is the CRISPR homing drive, which converts wild-type alleles to drive alleles in the germline of heterozygotes. Some potential target species are haplodiploid, in which males develop from unfertilized eggs and thus have only one copy of each chromosome. This prevents drive conversion, a substantial disadvantage compared to diploids where drive conversion can take place in both sexes. Here, we study the characteristics of homing suppression gene drives in haplodiploids and find that a drive targeting a female fertility gene could still be successful. However, such drives are less powerful than in diploids. They are substantially more vulnerable to high resistance allele formation in the embryo due to maternally deposited Cas9 and gRNA and also to somatic cleavage activity. Examining models of continuous space where organisms move over a landscape, we find that haplodiploid suppression drives surprisingly perform nearly as well as in diploids, possibly due to their ability to spread further before inducing strong suppression. Together, these results indicate that gene drive can potentially be used to effectively suppress haplodiploid populations.

Embracing multiculturalism of Canada: the roots & the present-day realities

2017 ◽  
pp. 32-41
Author(s):  
Valentyna Bohatyrets
Keyword(s):  
National Identity ◽  
Human Dignity ◽  
Public Support ◽  
Immigrant Integration ◽  
Mutual Understanding ◽  
Positive Outcomes ◽  
The World ◽  
The One ◽  
Digital Diplomacy ◽  
Equality And Diversity

The paper provides the framework for embracing multiculturalism as a source of national identity, a political ‘profession de foi’, and an engine for a government to gain positive outcomes, leading to better immigrant integration and economic advantages for any country in the world. Noteworthy, Canadian federal policy of multiculturalism, since its official adoption in 1971, is witnessed to work stunningly and in contrast to developments elsewhere – in Canada, public support for multiculturalism is seeing unprecedented growth. Currently, the diversity of the Canadian populace is increasing faster than at any time in its history; Canada’s ethnic makeup has notably altered over the time due to changing immigration patterns. According to the latest poll findings, 84% of Canadians agree with the statement that ‘Canada’s multicultural makeup is one of the best things about this country’; 61% of Canadians believe multiculturalism ‘strengthens national identity’. Moreover, released data from Environics reveals that 27% of Canadians believe ‘multiculturalism is the one characteristic about Canada that most deserves to be celebrated on its upcoming 150thanniversary. Undeniably, people around the world tend to view Canada as “good”. Importantly, the election of Justin Trudeau is viewed as an excellent opportunity to invigorate brand Canada. Noteworthy, brand Justin Trudeau is currently composed of his belief in and promotion of the values of tolerance, equality and diversity. While recognizing the value for society of the human dignity inherent in each individual, Trudeau’s government aims to push beyond mere tolerance to mutual understanding and respect. Keywords: Multiculturalism of Canada, immigration, digital diplomacy, brand, national identity, poll, ethnic groups

scholarly journals Novel combination of CRISPR-based gene drives eliminates resistance and localises spread

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nicky R. Faber ◽  
Gus R. McFarlane ◽  
R. Chris Gaynor ◽  
Ivan Pocrnic ◽  
C. Bruce A. Whitelaw ◽  
...  
Keyword(s):  
Driving Forces ◽  
Spatial Models ◽  
Cost Effective ◽  
Biodiversity Loss ◽  
Invasive Pest ◽  
Economic Losses ◽  
Sciurus Carolinensis ◽  
Gene Drive ◽  
Grey Squirrel ◽  
Gene Drives

AbstractInvasive species are among the major driving forces behind biodiversity loss. Gene drive technology may offer a humane, efficient and cost-effective method of control. For safe and effective deployment it is vital that a gene drive is both self-limiting and can overcome evolutionary resistance. We present HD-ClvR in this modelling study, a novel combination of CRISPR-based gene drives that eliminates resistance and localises spread. As a case study, we model HD-ClvR in the grey squirrel (Sciurus carolinensis), which is an invasive pest in the UK and responsible for both biodiversity and economic losses. HD-ClvR combats resistance allele formation by combining a homing gene drive with a cleave-and-rescue gene drive. The inclusion of a self-limiting daisyfield gene drive allows for controllable localisation based on animal supplementation. We use both randomly mating and spatial models to simulate this strategy. Our findings show that HD-ClvR could effectively control a targeted grey squirrel population, with little risk to other populations. HD-ClvR offers an efficient, self-limiting and controllable gene drive for managing invasive pests.

Potential impacts of poison baiting for introduced house mice on native animals on islands in Jurien Bay, Western Australia

2016 ◽  
Vol 43 (1) ◽  
pp. 61 ◽  
Author(s):  
Clifford Bennison ◽  
J. Anthony Friend ◽  
Timothy Button ◽  
Harriet Mills ◽  
Cathy Lambert ◽  
...  
Keyword(s):  
Western Australia ◽  
Rhodamine B ◽  
Native Species ◽  
House Mice ◽  
Mus Domesticus ◽  
Target Species ◽  
Island Populations ◽  
Delivery Technique ◽  
Poison Baiting ◽  
Competition For Resources

Context House mice (Mus domesticus) are present on Boullanger and Whitlock islands, Western Australia, and could potentially threaten populations of the dibbler (Parantechinus apicalis) and grey-bellied dunnart (Sminthopsis griseoventer) through competition for resources. A workshop in 2007 recommended a study to assess the feasibility of eradicating house mice from the islands by using poison baits and of the risk posed to non-target native species. Aim We aimed to assess the risk to non-target native species if poison baiting was used to eradicate house mice on Boullanger and Whitlock islands. Methods Non-toxic baits containing the bait marker rhodamine B were distributed on Boullanger Island and on the mouse free Escape Island to determine the potential for primary poisoning. Acceptance of baits by mammals was measured through sampling and analysis of whiskers, and by reptiles through observations of dye in faeces. To determine the potential for secondary exposure to poison, the response of dibblers to mouse carcasses was observed using motion-activated cameras. Bait acceptance was compared using two methods of delivery, namely, scattering in the open and delivery in polyvinyl chloride (PVC) tubes. A cafeteria experiment of bait consumption by dibblers was also undertaken using captive animals held at the Perth Zoo. Ten dibblers were offered non-toxic baits containing rhodamine B in addition to their normal meals; consumption of bait and the presence of dye in whiskers were measured. Key results Bait acceptance on the islands was high for house mice (92% of individuals) and dibblers (48%) and it was independent of bait-delivery technique. There was no evidence of bait acceptance by grey-bellied dunnarts. Dibblers may consume mice carcasses if available; however, no direct consumption of mice carcasses was observed with movement sensor cameras but one dibbler was observed removing a mouse carcass and taking it away. During the cafeteria experiment, 9 of 10 captive dibblers consumed baits. Conclusions This investigation demonstrated that dibblers consume baits readily and island populations would experience high mortality if exposed to poison baits. Poison baiting could effectively eradicate mice from Boullanger and Whitlock islands but not without mortality for dibblers. Implications Toxic baits could be used to eradicate mice from Boullanger and Whitlock islands, provided that non-target species such as dibblers were temporarily removed from the islands before the application of baits.

scholarly journals Toxicity of neem's oil, a potential biocide against the invasive mussel Limnoperna fortunei (Dunker 1857)

2012 ◽  
Vol 84 (4) ◽  
pp. 1065-1071 ◽  
Author(s):  
Patricio J. Pereyra ◽  
Gustavo B. Rossini ◽  
Gustavo Darrigran
Keyword(s):  
Invasive Species ◽  
Toxicity Tests ◽  
Limnoperna Fortunei ◽  
Target Species ◽  
Potential Toxicity ◽  
A Value ◽  
Closed Systems ◽  
The One ◽  
Invasive Mussel ◽  
Golden Mussel

The golden mussel Limnoperna fortunei (Dunker 1857) is one of the most distributed Nuisance Invasive Species (NIS) in South America, and a threat of great concern for the industry of the area. In this study, we carried out toxicity tests made with a Neem's oil solution with L. fortunei larvae and benthonic adults (7, 13 and 19 ± 1 mm). Tests with non-target species (Daphnia magna, Lactuca sativa and Cnesterodon decemmculatus) were also made with the aim to evaluate the potential toxicity of the Neem's solution in the environment. The LC100 of Neem's solution obtained for larvae was 500 µl/L, a value much higher than the one obtained for D. magna and C. decemmaculatus. Thus, we recommend that it should not be used in open waters. However, since the adults were killed in 72 h and the larvae in 24 h, this product can be used in closed systems, in man-made facilities.

scholarly journals Priority Treatment Leaves Grassland Restoration Vulnerable to Invasion

Diversity ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 71 ◽  
Author(s):  
Katharine L. Stuble ◽  
Truman P. Young
Keyword(s):  
Native Species ◽  
Priority Effects ◽  
Native Grasses ◽  
Target Species ◽  
California Grasslands ◽  
Early Arrival ◽  
Regional Species Pool ◽  
Conservation Concern ◽  
Indirect Impacts ◽  
One Year

Priority effects can be used to promote target species during restoration. Early planting can provide an advantage over later-arriving species, increasing abundance of these early-arrivers in restored communities. However, we have limited knowledge of the indirect impacts of priority effects in restoration. In particular, we do not understand how priority effects impact non-target species. Of particular conservation concern is how these priority effects influence establishment by non-native species. We use a field-based mesocosm experiment to explore the impacts of priority effects on both target and non-target species in California grasslands. Specifically, we seeded native grasses and forbs, manipulating order of arrival by planting them at the same time, planting forbs one year before grasses, planting grasses one year before forbs, or planting each functional group alone. While our study plots were tilled and weeded for the first year, the regional species pool was heavily invaded. We found that, while early-arrival of native grasses did not promote establishment of non-native species, giving priority to native forbs ultimately left our restoration mesocosms vulnerable to invasion by non-native species. This suggests that, in some cases, establishment of non-native species may be an unintended consequence of using priority treatments as a restoration tool.

scholarly journals Making Smart and Sustainable Infrastructure Projects Viable: Private Choices, Public Support, and Systems Constraints

2017 ◽  
Vol 2 (3) ◽  
pp. 18-32 ◽  
Author(s):  
Douglas Noonan ◽  
Shan Zhou ◽  
Robert Kirkman
Keyword(s):  
Public Perception ◽  
Public Support ◽  
Green Technologies ◽  
Public And Private ◽  
Private Interests ◽  
Public Survey ◽  
Private And Public ◽  
Infrastructure Investments ◽  
Individual Use ◽  
The One

Sustainable cities will require major infrastructure investments coupled with widespread behavioral change. Examples of smart, green technologies abound, but evidence for actual use lags. This partly owes to the tension between public support and private choices: individuals thinking as members of the public may see solutions as smart for the city, but thinking of their private interests may see those same solutions as not smart for themselves. This also owes to the disconnect between private and public choices, on the one hand, and the workings of complex systems, on the other. Even if public and private interests align, existing built environment systems may resist change. This article examines public perception and use of the Atlanta BeltLine, a pioneering sustainability initiative to transform the auto-dependent city into a greener, denser city. Analyzing a general public survey reveals widespread support for the BeltLine alongside reticence from residents to change their commute or greenspace use. The findings also show that drivers of public support and prospective use of the BeltLine differ. Public support may be insufficient if individual use decisions do not follow. Yet, private adoption decisions may not follow until and unless the systems in which they are embedded are already changing.

Why They Fight: How Perceived Motivations for Military Service Shape Support for the Use of Force

2021 ◽  
Author(s):  
Ronald R Krebs ◽  
Robert Ralston ◽  
Aaron Rapport
Keyword(s):  
Military Service ◽  
Cost Benefit Analysis ◽  
Public Support ◽  
Cost Benefit ◽  
Good Citizen ◽  
Political Partisanship ◽  
Nationally Representative ◽  
And Gender ◽  
The One ◽  
The Relationship

Abstract What shapes public support for military missions? Existing scholarship points to, on the one hand, individuals’ affiliations and predispositions (such as political partisanship and gender), and, on the other hand, factors that shape a rational cost–benefit analysis (notably, mission objectives, the prospects for victory, and the magnitude and distribution of costs). We argue that public opinion is also shaped by beliefs about why soldiers voluntarily enlist. Using novel survey data and an experiment, deployed to a nationally representative sample of Americans, we test how four conceptions of soldiering affect support for a prospective military operation. We find, in observational data, that believing that a soldier is a good citizen or patriot bolsters support for the mission, while believing that a soldier has enlisted because he wants the material benefits of service or has “no other options” undermines support. These results support our causal argument: Americans’ attitudes toward military missions are shaped by their perception of whether the soldier has consented to deployment rather than by feelings of social obligation. This article has implications for debates on the determinants of public support for military missions and the relationship between military service and citizenship in democracies.

Towards CRISPR/Cas9-based gene drive in the diamondback moth Plutella xylostella

2021 ◽  
Author(s):  
Xuejiao Xu ◽  
Tim Harvey-Samuel ◽  
Hamid Anees Siddiqui ◽  
Joshua Ang ◽  
Michelle E Anderson ◽  
...  
Keyword(s):  
Genetic Control ◽  
Plutella Xylostella ◽  
Control Strategies ◽  
Diamondback Moth ◽  
Regulatory Elements ◽  
Strategy Development ◽  
Gene Drive ◽  
Global Agriculture ◽  
High Level ◽  
Gene Drives

Promising to provide powerful genetic control tools, gene drives have been constructed in multiple dipterans, yeast and mice, for the purposes of population elimination or modification. However, it remains unclear whether these techniques can be applied to lepidopterans. Here, we used endogenous regulatory elements to drive Cas9 and sgRNA expression in the diamondback moth, (Plutella xylostella), and test the first split-drive system in a lepidopteran. The diamondback moth is an economically important global agriculture pest of cruciferous crops and has developed severe resistance to various insecticides, making it a prime candidate for such novel control strategy development. A very high level of somatic editing was observed in Cas9/sgRNA transheterozygotes, although no significant homing was revealed in the subsequent generation. Although heritable, Cas9-medated germline cleavage, as well as maternal and paternal Cas9 deposition was observed, rates were far lower than for somatic cleavage events, indicating robust somatic but limited germline activity of Cas9/sgRNA under the control of selected regulatory elements. Our results provide valuable experience, paving the way for future construction of gene drive-based genetic control strategies in DBM or other lepidopterans.

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