scholarly journals Impaired DNA replication derepresses chromatin and generates a transgenerationally inherited epigenetic memory

2017 ◽  
Vol 3 (8) ◽  
pp. e1701143 ◽  
Author(s):  
Adam Klosin ◽  
Kadri Reis ◽  
Cristina Hidalgo-Carcedo ◽  
Eduard Casas ◽  
Tanya Vavouri ◽  
...  
Keyword(s):  
Dna Replication ◽  
Epigenetic Memory

scholarly journals Epigenetic Memory Independent of Symmetric Histone Inheritance

2019 ◽  
Author(s):  
Daniel S Saxton ◽  
Jasper Rine
Keyword(s):  
Gene Regulation ◽  
Dna Replication ◽  
Gene Silencing ◽  
Histone Modifications ◽  
Epigenetic Memory ◽  
Important Form

AbstractHeterochromatic gene silencing is an important form of gene regulation that usually requires specific histone modifications. A popular model posits that inheritance of modified histones, especially in the form of H3-H4 tetramers, underlies inheritance of heterochromatin. Because H3-H4 tetramers are randomly distributed between daughter chromatids during DNA replication, rare occurrences of asymmetric tetramer inheritance within a heterochromatic domain would have the potential to destabilize heterochromatin. This model makes a prediction that shorter heterochromatic domains would experience unbalanced tetramer inheritance more frequently, and thereby be less stable. In contrast to this prediction, we found that shortening a heterochromatic domain in Saccharomyces had no impact on the strength of silencing nor its heritability. Additionally, we found that replisome mutations that disrupt inheritance of H3-H4 tetramers had only minor effects on heterochromatin stability. These findings suggest that histones carry little or no memory of the heterochromatin state through DNA replication.

scholarly journals Epigenetic memory independent of symmetric histone inheritance

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Daniel S Saxton ◽  
Jasper Rine
Keyword(s):  
Gene Regulation ◽  
Dna Replication ◽  
Gene Silencing ◽  
Histone Modifications ◽  
Epigenetic Memory ◽  
Important Form

Heterochromatic gene silencing is an important form of gene regulation that usually requires specific histone modifications. A popular model posits that inheritance of modified histones, especially in the form of H3-H4 tetramers, underlies inheritance of heterochromatin. Because H3-H4 tetramers are randomly distributed between daughter chromatids during DNA replication, rare occurrences of asymmetric tetramer inheritance within a heterochromatic domain would have the potential to destabilize heterochromatin. This model makes a prediction that shorter heterochromatic domains would experience unbalanced tetramer inheritance more frequently, and thereby be less stable. In contrast to this prediction, we found that shortening a heterochromatic domain in Saccharomyces had no impact on the strength of silencing nor its heritability. Additionally, we found that replisome mutations that disrupt inheritance of H3-H4 tetramers had only minor effects on heterochromatin stability. These findings suggest that histones carry little or no memory of the heterochromatin state through DNA replication.

Replication of Bacteriophage 186 DNA

1972 ◽  
Vol 30 ◽  
pp. 194-195
Author(s):  
Dhruba K. Chattoraj ◽  
Ross B. Inman
Keyword(s):  
Electron Microscopy ◽  
Dna Replication ◽  
Frame Of Reference ◽  
Dna Molecules ◽  
E Coli ◽  
Phage Dna ◽  
Partial Denaturation

Electron microscopy of replicating intermediates has been quite useful in understanding the mechanism of DNA replication in DNA molecules of bacteriophage, mitochondria and plasmids. The use of partial denaturation mapping has made the tool more powerful by providing a frame of reference by which the position of the replicating forks in bacteriophage DNA can be determined on the circular replicating molecules. This provided an easy means to find the origin and direction of replication in λ and P2 phage DNA molecules. DNA of temperate E. coli phage 186 was found to have an unique denaturation map and encouraged us to look into its mode of replication.

The infection and viral DNA replication ofBocavirusminute virus of canine in permissive cells and non-permissive cells

2010 ◽  
Vol 34 (8) ◽  
pp. S60-S60
Author(s):  
Yuning Sun ◽  
Fang Li ◽  
Jianming Qiu ◽  
Xiaohong Lu
Keyword(s):  
Dna Replication ◽  
Viral Dna ◽  
Viral Dna Replication

DNA replication and the development of preprophase bands in soybean protoplast cultures

1991 ◽  
Vol 82 (2) ◽  
pp. 150-156 ◽  
Author(s):  
Hong Wang ◽  
Adrian J. Cutler ◽  
Larry C. Fowke
Keyword(s):  
Dna Replication ◽  
Preprophase Bands
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