scholarly journals Chemotactic synthetic vesicles: Design and applications in blood-brain barrier crossing

2017 ◽  
Vol 3 (8) ◽  
pp. e1700362 ◽  
Author(s):  
Adrian Joseph ◽  
Claudia Contini ◽  
Denis Cecchin ◽  
Sophie Nyberg ◽  
Lorena Ruiz-Perez ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Barrier Crossing ◽  
Blood Brain

scholarly journals Trojan Horse Transit Contributes to Blood-Brain Barrier Crossing of a Eukaryotic Pathogen

mBio ◽  
2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Felipe H. Santiago-Tirado ◽  
Michael D. Onken ◽  
John A. Cooper ◽  
Robyn S. Klein ◽  
Tamara L. Doering
Keyword(s):  
Experimental Evidence ◽  
Cryptococcus Neoformans ◽  
Blood Brain Barrier ◽  
Fungal Pathogen ◽  
Trojan Horse ◽  
Brain Barrier ◽  
Barrier Crossing ◽  
Blood Brain ◽  
The Brain

ABSTRACT The blood-brain barrier (BBB) protects the central nervous system (CNS) by restricting the passage of molecules and microorganisms. Despite this barrier, however, the fungal pathogen Cryptococcus neoformans invades the brain, causing a meningoencephalitis that is estimated to kill over 600,000 people annually. Cryptococcal infection begins in the lung, and experimental evidence suggests that host phagocytes play a role in subsequent dissemination, although this role remains ill defined. Additionally, the disparate experimental approaches that have been used to probe various potential routes of BBB transit make it impossible to assess their relative contributions, confounding any integrated understanding of cryptococcal brain entry. Here we used an in vitro model BBB to show that a “Trojan horse” mechanism contributes significantly to fungal barrier crossing and that host factors regulate this process independently of free fungal transit. We also, for the first time, directly imaged C. neoformans-containing phagocytes crossing the BBB, showing that they do so via transendothelial pores. Finally, we found that Trojan horse crossing enables CNS entry of fungal mutants that cannot otherwise traverse the BBB, and we demonstrate additional intercellular interactions that may contribute to brain entry. Our work elucidates the mechanism of cryptococcal brain invasion and offers approaches to study other neuropathogens. IMPORTANCE The fungal pathogen Cryptococcus neoformans invades the brain, causing a meningoencephalitis that kills hundreds of thousands of people each year. One route that has been proposed for this brain entry is a Trojan horse mechanism, whereby the fungus crosses the blood-brain barrier (BBB) as a passenger inside host phagocytes. Although indirect experimental evidence supports this intriguing mechanism, it has never been directly visualized. Here we directly image Trojan horse transit and show that it is regulated independently of free fungal entry, contributes to cryptococcal BBB crossing, and allows mutant fungi that cannot enter alone to invade the brain. IMPORTANCE The fungal pathogen Cryptococcus neoformans invades the brain, causing a meningoencephalitis that kills hundreds of thousands of people each year. One route that has been proposed for this brain entry is a Trojan horse mechanism, whereby the fungus crosses the blood-brain barrier (BBB) as a passenger inside host phagocytes. Although indirect experimental evidence supports this intriguing mechanism, it has never been directly visualized. Here we directly image Trojan horse transit and show that it is regulated independently of free fungal entry, contributes to cryptococcal BBB crossing, and allows mutant fungi that cannot enter alone to invade the brain.

Accumulation of Eu3+ chelates in cells expressing or not P-glycoprotein: Implications for blood–brain barrier crossing

2010 ◽  
Vol 104 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Nacira Darghal ◽  
Arlette Garnier-Suillerot ◽  
Nadia Bouchemal ◽  
Gabriel Gras ◽  
Carlos F.G.C. Geraldes ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Barrier Crossing ◽  
P Glycoprotein ◽  
Blood Brain ◽  
In Cells

scholarly journals Remarkably Efficient Blood–Brain Barrier Crossing Polyfluorene–Chitosan Nanoparticle Selectively Tweaks Amyloid Oligomer in Cerebrospinal Fluid and Aβ1–40

ACS Omega ◽  
2018 ◽  
Vol 3 (7) ◽  
pp. 8059-8066 ◽  
Author(s):  
Sayan Roy Chowdhury ◽  
Subrata Mondal ◽  
Balakrishnan Muthuraj ◽  
S. N. Balaji ◽  
Vishal Trivedi ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Barrier Crossing ◽  
Blood Brain ◽  
Chitosan Nanoparticle ◽  
Amyloid Oligomer

Emerging blood–brain-barrier-crossing nanotechnology for brain cancer theranostics

2019 ◽  
Vol 48 (11) ◽  
pp. 2967-3014 ◽  
Author(s):  
Wei Tang ◽  
Wenpei Fan ◽  
Joseph Lau ◽  
Liming Deng ◽  
Zheyu Shen ◽  
...  
Keyword(s):  
Brain Tumor ◽  
Blood Brain Barrier ◽  
Brain Cancer ◽  
Brain Barrier ◽  
Barrier Crossing ◽  
Highly Efficient ◽  
Blood Brain ◽  
Tumor Delivery ◽  
Cancer Theranostics

The advancements, perspectives, and challenges in blood–brain-barrier (BBB)-crossing nanotechnology for effective brain tumor delivery and highly efficient brain cancer theranostics.

Sign in / Sign up

Export Citation Format

Share Document