scholarly journals HIV virions sense plasma membrane heterogeneity for cell entry

2017 ◽  
Vol 3 (6) ◽  
pp. e1700338 ◽  
Author(s):  
Sung-Tae Yang ◽  
Alex J. B. Kreutzberger ◽  
Volker Kiessling ◽  
Barbie K. Ganser-Pornillos ◽  
Judith M. White ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Cell Entry ◽  
Membrane Heterogeneity ◽  
Plasma Membrane Heterogeneity

Effects of different detachment procedures on viability, nitroxide reduction kinetics and plasma membrane heterogeneity of V-79 cells

2010 ◽  
Vol 34 (6) ◽  
pp. 663-668 ◽  
Author(s):  
Urška Batista ◽  
Maja Garvas ◽  
Marjana Nemec ◽  
Milan Schara ◽  
Peter Veranič ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Reduction Kinetics ◽  
Membrane Heterogeneity ◽  
Plasma Membrane Heterogeneity

scholarly journals Minimal Model of Plasma Membrane Heterogeneity Requires Coupling Cortical Actin to Criticality

2011 ◽  
Vol 100 (7) ◽  
pp. 1668-1677 ◽  
Author(s):  
Benjamin B. Machta ◽  
Stefanos Papanikolaou ◽  
James P. Sethna ◽  
Sarah L. Veatch
Keyword(s):  
Plasma Membrane ◽  
Minimal Model ◽  
Cortical Actin ◽  
Membrane Heterogeneity ◽  
Plasma Membrane Heterogeneity

What do nanometer molecular trajectories tell us about heterogeneous cell surface domaines?

1995 ◽  
Vol 53 ◽  
pp. 786-787
Author(s):  
Watt W. Webb
Keyword(s):  
Cell Surface ◽  
Lipid Membranes ◽  
Surface Proteins ◽  
Protein Diffusion ◽  
Coated Pits ◽  
Cell Surface Proteins ◽  
Membrane Heterogeneity ◽  
Fluorescence Photobleaching Recovery ◽  
Photobleaching Recovery ◽  
Plasma Membrane Heterogeneity

Plasma membrane heterogeneity is implicit in the existence of specialized cell surface organelles which are necessary for cellular function; coated pits, post and pre-synaptic terminals, microvillae, caveolae, tight junctions, focal contacts and endothelial polarization are examples. The persistence of these discrete molecular aggregates depends on localized restraint of the constituent molecules within specific domaines in the cell surface by strong intermolecular bonds and/or anchorage to extended cytoskeleton. The observed plasticity of many of organelles and the dynamical modulation of domaines induced by cellular signaling evidence evanescent intermolecular interactions even in conspicuous aggregates. There is also strong evidence that universal restraints on the mobility of cell surface proteins persist virtually everywhere in cell surfaces, not only in the discrete organelles. Diffusion of cell surface proteins is slowed by several orders of magnitude relative to corresponding protein diffusion coefficients in isolated lipid membranes as has been determined by various ensemble average methods of measurement such as fluorescence photobleaching recovery(FPR).

scholarly journals Stabilizing membrane domains antagonizes n-alcohol anesthesia

2016 ◽  
Author(s):  
B.B. Machta ◽  
E. Grey ◽  
M. Nouri ◽  
N.L.C. McCarthy ◽  
E.M. Gray ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Phase Separation ◽  
Critical Temperature ◽  
Hydrostatic Pressure ◽  
General Anesthesia ◽  
Membrane Domains ◽  
Strongly Correlated ◽  
Membrane Heterogeneity ◽  
Anesthetic Potency ◽  
Better Than

AbstractDiverse molecules induce general anesthesia with potency strongly correlated both with their hydrophobicity and their effects on certain ion channels. We recently observed that several n-alcohol anesthetics inhibit heterogeneity in plasma membrane derived vesicles by lowering the critical temperature (Tc) for phase separation. Here we exploit conditions that stabilize membrane heterogeneity to further test the correlation between the anesthetic potency of n-alcohols and effects on Tc. First we show that hexadecanol acts oppositely to n-alcohol anesthetics on membrane mixing and antagonizes ethanol induced anesthesia in a tadpole behavioral assay. Second, we show that two previously described ‘intoxication reversers’ raise Tc and counter ethanol’s effects in vesicles, mimicking the findings of previous electrophysiological and behavioral measurements. Third, we find that hydrostatic pressure, long known to reverse anesthesia, also raises Tc in vesicles with a magnitude that counters the effect of butanol at relevant concentrations and pressures. Taken together,these results demonstrate that ΔTc predicts anesthetic potency for n-alcohols better than hydrophobicity in a range of contexts, supporting a mechanistic role for membrane heterogeneity in general anesthesia.

scholarly journals Membrane Heterogeneity Beyond the Plasma Membrane

2020 ◽  
Vol 8 ◽  
Author(s):  
Hong-Yin Wang ◽  
Deepti Bharti ◽  
Ilya Levental
Keyword(s):  
Plasma Membrane ◽  
Membrane Heterogeneity

scholarly journals The broad anti-viral agent glycyrrhizin directly modulates the fluidity of plasma membrane and HIV-1 envelope

2005 ◽  
Vol 392 (1) ◽  
pp. 191-199 ◽  
Author(s):  
Shinji Harada
Keyword(s):  
Plasma Membrane ◽  
Membrane Fluidity ◽  
Viral Envelope ◽  
Cell Entry ◽  
Cell Leukaemia ◽  
Fusion Pore ◽  
Type I ◽  
Viral Agent ◽  
Leukaemia Virus ◽  
Hiv 1

Cell entry of enveloped viruses requires a wide-fusion-pore mechanism, involving clustering of fusion-activated proteins and fluidization of the plasma membrane and viral envelope. In the present study, GL (glycyrrhizin) is reported to lower membrane fluidity, thus suppressing infection by HIV, influenza A virus and vesicular stomatitis virus, but not by poliovirus. GL-treated HIV-1 particles showed reduced infectivity. GL also inhibited cell-to-cell fusion induced by HIV-1 and HTLV-I (human T-cell leukaemia virus type I). However, when cells treated with 1 mg/ml GL were placed in GL-free medium, they showed increased susceptibility to HIV-1 infection and HTLV-I fusion due to enhancement of membrane fluidity. The membrane dependence of GL and GL removal experiments suggest that GL does affect the cell entry of viruses. HIVs with more gp120 were less dependent on temperature and less sensitive to GL treatment than those with less gp120, indicating that the existence of more gp120 molecules resulted in a higher probability of forming a cluster of fusion-activated proteins.

The target cell plasma membrane is a critical interface for Salmonella cell entry effector-host interplay

2004 ◽  
Vol 54 (4) ◽  
pp. 887-904 ◽  
Author(s):  
Robert J. Cain ◽  
Richard D. Hayward ◽  
Vassilis Koronakis
Keyword(s):  
Plasma Membrane ◽  
Target Cell ◽  
Cell Entry ◽  
Cell Plasma Membrane ◽  
Cell Plasma
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