Prostaglandin E2Inhibits the Phospholipase D Pathway Stimulated by Formyl-methionyl-leucyl-phenylalanine in Human Neutrophils. Involvement of EP2Receptors and Phosphatidylinositol 3-kinase γ

2004 ◽  
Vol 66 (2) ◽  
pp. 293-301 ◽  
Author(s):  
Chantal Burelout ◽  
Nathalie Thibault ◽  
Sylvain Levasseur ◽  
Sébastien Simard ◽  
Paul H. Naccache ◽  
...  
Keyword(s):  
Phospholipase D ◽  
Human Neutrophils ◽  
Phosphatidylinositol 3 Kinase ◽  
Phosphatidylinositol 3

scholarly journals Control of the Oxidative Burst of Human Neutrophils by Staphylococcal Leukotoxins

2003 ◽  
Vol 71 (7) ◽  
pp. 3724-3729 ◽  
Author(s):  
Didier A. Colin ◽  
Henri Monteil
Keyword(s):  
Flow Cytometry ◽  
Oxidative Burst ◽  
Pore Formation ◽  
Human Neutrophils ◽  
Polymorphonuclear Cells ◽  
Phosphatidylinositol 3 Kinase ◽  
Intracellular Release ◽  
Two Component ◽  
Phosphatidylinositol 3

ABSTRACT The ability of staphylococcal two-component leukotoxins to induce an oxidative burst and/or to prime human polymorphonuclear cells (PMNs) was studied by using spectrofluorometry or flow cytometry. At sublytic concentrations, the HlgA-HlgB, HlgA-LukF-PV, LukS-PV-LukF-PV, and HlgC-LukF-PV combinations of leukotoxins, but not the LukS-PV-HlgB and HlgC-HlgB combinations, were able to induce H2O2 production similar to the H2O2 production induced by 1 μM N-formyl-Met-Leu-Phe (fMLP). In addition, when added at sublytic concentrations, all of the leukotoxin combinations primed PMNs for H2O2 production induced by fMLP. Leukotoxin activation was dependent on the presence of Ca2+ and was inhibited by wortmannin, an inhibitor of phosphatidylinositol 3-kinase, but not by N-methyl-l-arginine, an inhibitor of NO generation, which eliminates the possibility that NO plays a role in the action of leukotoxins. At higher concentrations, all leukotoxins inhibited H2O2 production by PMNs activated by fMLP, phorbol 12-myristate 13-acetate (PMA), or the leukotoxins themselves. This inhibition was not related to the pore formation induced by leukotoxins. Intracellular release of H2O2 induced by fMLP and PMA was not primed by leukotoxins but was inhibited. It seems that leukotoxin inhibition of H2O2 release is independent of pore formation but secondary to an intracellular event, as yet unknown, triggered by leukotoxins.

scholarly journals C5a delays apoptosis of human neutrophils by a phosphatidylinositol 3-kinase-signaling pathway

2002 ◽  
Vol 61 (2) ◽  
pp. 456-463 ◽  
Author(s):  
Mary C. Perianayagam ◽  
V.S. Balakrishnan ◽  
Andrew J. King ◽  
Brian J.G. Pereira ◽  
Bertrand L. Jaber
Keyword(s):  
Signaling Pathway ◽  
Human Neutrophils ◽  
Kinase Signaling ◽  
Phosphatidylinositol 3 Kinase ◽  
Kinase Signaling Pathway ◽  
Phosphatidylinositol 3
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