Specificity of G Protein-Coupled Receptor Kinase 6-Mediated Phosphorylation and Regulation of Single-Cell M3 Muscarinic Acetylcholine Receptor Signaling

2003 ◽  
Vol 64 (5) ◽  
pp. 1059-1068 ◽  
Author(s):  
Jonathon M. Willets ◽  
Rajendra Mistry ◽  
Stefan R. Nahorski ◽  
R. A. John Challiss
Keyword(s):  
Single Cell ◽  
G Protein ◽  
Acetylcholine Receptor ◽  
Muscarinic Acetylcholine Receptor ◽  
Receptor Signaling ◽  
Receptor Kinase ◽  
G Protein Coupled Receptor ◽  
Muscarinic Acetylcholine ◽  
M3 Muscarinic Acetylcholine Receptor ◽  
G Protein Coupled

Internalization of the M2 muscarinic acetylcholine receptor proceeds through an atypical pathway in HEK293 cells that is independent of clathrin and caveolae

2001 ◽  
Vol 114 (4) ◽  
pp. 739-746 ◽  
Author(s):  
A.G. Roseberry ◽  
M.M. Hosey
Keyword(s):  
G Protein ◽  
Acetylcholine Receptor ◽  
Muscarinic Acetylcholine Receptor ◽  
Hek293 Cells ◽  
G Protein Coupled Receptor ◽  
Coated Pits ◽  
Muscarinic Acetylcholine ◽  
G Protein Coupled

The M2 muscarinic acetylcholine receptor is a G-protein coupled receptor that undergoes agonist-induced internalization through an unidentified pathway that exhibits an atypical dependence on dynamin function in HEK293 cells. In this report we utilized several independent approaches to reveal that the internalization of the M2 muscarinic acetylcholine receptor did not utilize clathrin-coated pits or caveolae. However, we did observe that treatment with hypertonic sucrose, which is widely reported to specifically inhibit endocytosis through clathrin-coated pits, completely inhibited internalization of the M2 muscarinic acetylcholine receptor. Thus, the pathway that mediates the internalization of the M2 muscarinic acetylcholine receptor appears to be atypical in that it exhibits an unusual sensitivity to dynamin and is inhibited by hypertonic sucrose but lacks the involvement of clathrin and caveolae.

scholarly journals Regulation of β-Adrenergic Receptor Signaling by S-Nitrosylation of G-Protein-Coupled Receptor Kinase 2

Cell ◽  
2007 ◽  
Vol 129 (3) ◽  
pp. 511-522 ◽  
Author(s):  
Erin J. Whalen ◽  
Matthew W. Foster ◽  
Akio Matsumoto ◽  
Kentaro Ozawa ◽  
Jonathan D. Violin ◽  
...  
Keyword(s):  
G Protein ◽  
Adrenergic Receptor ◽  
Receptor Signaling ◽  
Receptor Kinase ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled
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