Genotoxic Profiling of MCF-7 Breast Cancer Cell Line Elucidates Gene Expression Modifications Underlying Toxicity of the Anticancer Drug 2-(4-Amino-3-methylphenyl)-5-fluorobenzothiazole

2003 ◽  
Vol 63 (3) ◽  
pp. 766-772 ◽  
Author(s):  
Anne Monks ◽  
Erik Harris ◽  
Curtis Hose ◽  
John Connelly ◽  
Edward A. Sausville
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Anticancer Drug ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Mcf 7

scholarly journals Apoptosis Induced by Melatonin and Vitamin D3 in MCF-7 Breast Cancer Cell Line by Upregulating the Gene Expression of P53

2020 ◽  
Author(s):  
Abir Alamro ◽  
Nora Alkeraishan ◽  
Manal AlMalky ◽  
Amani Alghamdi ◽  
Nasser M Al-Daghri ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Vitamin D3 ◽  
Breast Cancer Cell Line ◽  
Combined Treatment ◽  
Cancer Cell Line ◽  
Mcf 7

Abstract Background: P53 was identified as the first tumor suppressor gene actively involved in numerous cellular mechanisms such as initiating DNA repair mechanisms, apoptosis, and cell cycle arrest. More than 50% of all human cancers have a mutated nonfunctional p53 expression. Breast cancer (BC) is one of the leading causes of mortality in females and mutated p53 is documented to be the causative agent in only 20% of them. However, a mutation in p53 in BC results in a more aggressive form of cancer which is more resistant to conventional therapies. Recently multiple clinical trials suggested that the combined use of melatonin and vitamin D3 can slow down the growth of breast cancer cells. The genetic and molecular mechanisms through which these compounds initiate the cancerous cells to apoptosis or cell cycle arrest is still not fully understood. This study aims to investigate the effect of melatonin, vitamin D3, and their combined treatment on the gene expression of P53 in MCF-7 breast cancer cell line. Methods: The optimum treatment dose for melatonin and vitamin D3 were found by MTT and LDH assay. MCF-7 breast cancer cell line were treated with this optimum concentration, and studied the gene expression of p53 by RTPCR and concomitant protein expression by western blots analysisResults: This study indicated that 5nM melatonin, 0.5nM vitamin D3, and their combined treatment inhibited the proliferation of MCF-7 breast cancer cells and caused apoptosis by upregulating the p53 gene expression (2-fold) and significant increase (p<0.001) in p53 protein expression. Conclusion: Treatments with melatonin and vitamin D3 were found to have anti-cancerous effect and could potentially prove to be the ideal natural adjuvant therapy for the treatment of breast cancer.

scholarly journals The effects of melatonin and vitamin D3 on the gene expression of BCl-2 and BAX in MCF-7 breast cancer cell line

2021 ◽  
Vol 33 (2) ◽  
pp. 101287
Author(s):  
Abir A. Alamro ◽  
Manal M. Al-Malky ◽  
Mohammed G.A. Ansari ◽  
Osama E. Amer ◽  
Abdullah M. Alnaami ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Vitamin D3 ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Mcf 7

Radiosensitizing and Phototherapeutic Effects of AuNPs are Mediated by Differential Noxa and Bim Gene Expression in MCF-7 Breast Cancer Cell Line

2021 ◽  
Vol 20 (1) ◽  
pp. 20-27
Author(s):  
Sameh Nakhla ◽  
Ayman Rahawy ◽  
Mohamed Abd El Salam ◽  
Thana Shalaby ◽  
Mohamed Zaghloul ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Mcf 7

scholarly journals Establishment and characterization of a new spontaneously immortalized ER−/PR−/HER2+ human breast cancer cell line, DHSF-BR16

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Stefania Nobili ◽  
Antonella Mannini ◽  
Astrid Parenti ◽  
Chiara Raggi ◽  
Andrea Lapucci ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Biology ◽  
Cancer Cell Line ◽  
Cancer Tissue ◽  
Endoplasmic Reticulum Membrane ◽  
Mcf 7

AbstractInvasive ductal carcinoma (IDC) constitutes the most frequent malignant cancer endangering women’s health. In this study, a new spontaneously immortalized breast cancer cell line, DHSF-BR16 cells, was isolated from the primary IDC of a 74-years old female patient, treated with neoadjuvant chemotherapy and disease-free 5-years after adjuvant chemotherapy. Primary breast cancer tissue surgically removed was classified as ER−/PR−/HER2+, and the same phenotype was maintained by DHSF-BR16 cells. We examined DHSF-BR16 cell morphology and relevant biological and molecular markers, as well as their response to anticancer drugs commonly used for breast cancer treatment. MCF-7 cells were used for comparison purposes. The DHSF-BR16 cells showed the ability to form spheroids and migrate. Furthermore, DHSF-BR16 cells showed a mixed stemness phenotype (i.e. CD44+/CD24−/low), high levels of cytokeratin 7, moderate levels of cytokeratin 8 and 18, EpCAM and E-Cadh. Transcriptome analysis showed 2071 differentially expressed genes between DHSF-BR16 and MCF-7 cells (logFC > 2, p-adj < 0.01). Several genes were highly upregulated or downregulated in the new cell line (log2 scale fold change magnitude within − 9.6 to + 12.13). A spontaneous immortalization signature, mainly represented by extracellular exosomes-, plasma membrane- and endoplasmic reticulum membrane pathways (GO database) as well as by metabolic pathways (KEGG database) was observed in DHSF-BR16 cells. Also, these cells were more resistant to anthracyclines compared with MCF-7 cells. Overall, DHSF-BR16 cell line represents a relevant model useful to investigate cancer biology, to identify both novel prognostic and drug response predictive biomarkers as well as to assess new therapeutic strategies.

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