Analysis of a Phenobarbital-Responsive Enhancer Sequence Located in the 5′ Flanking Region of the ChickenCYP2H1Gene: Identification and Characterization of Functional Protein-Binding Sites

1999 ◽  
Vol 55 (1) ◽  
pp. 14-22 ◽  
Author(s):  
Satish C. Dogra ◽  
Benjamin P. Davidson ◽  
Brian K. May
Keyword(s):  
Protein Binding ◽  
Binding Sites ◽  
Functional Protein ◽  
Protein Binding Sites ◽  
Enhancer Sequence ◽  
Flanking Region ◽  
Identification And Characterization

Activation of Platinum(IV) Prodrugs by Cytochrome c and Characterization of the Protein Binding Sites

2016 ◽  
Vol 13 (9) ◽  
pp. 3216-3223 ◽  
Author(s):  
Alessia Lasorsa ◽  
Olga Stuchlíková ◽  
Viktor Brabec ◽  
Giovanni Natile ◽  
Fabio Arnesano
Keyword(s):  
Cytochrome C ◽  
Protein Binding ◽  
Binding Sites ◽  
Protein Binding Sites

scholarly journals Protein-binding sites within the 5' DNase I-hypersensitive region of the chicken alpha D-globin gene.

1987 ◽  
Vol 7 (6) ◽  
pp. 2059-2069 ◽  
Author(s):  
B Kemper ◽  
P D Jackson ◽  
G Felsenfeld
Keyword(s):  
Protein Binding ◽  
Binding Sites ◽  
Globin Gene ◽  
Regulatory Elements ◽  
Dnase I ◽  
Specific Factor ◽  
Mobility Shift ◽  
Protein Binding Sites ◽  
Dnase I Footprinting ◽  
Flanking Region

We mapped at high resolution and as a function of development the hypersensitive domain in the 5'-flanking region of the chicken alpha D-globin gene and determined the specific protein-binding sites within the domain. The domain extends from -130 to +80 nucleotides (nt) relative to the cap site. DNase I footprinting within intact embryonic erythrocyte nuclei revealed a strongly protected area from -71 to -52 nt. The same area was weakly protected in adult nuclei. A factor was present in extracts of erythrocyte nuclei from both embryos and adults that protected the sequence AAGATAAGG (-63 to -55 nt) in DNase I footprinting experiments; at higher concentrations of extract, sequences immediately adjacent (-73 to -64 and -53 to -38) were also protected. The same pattern of binding was revealed by gel mobility shift assays. The identical AAGATAAGG sequence is found in the 5'-flanking region of the beta rho gene; it competed for binding of the alpha D-specific factor, suggesting that regulatory elements are shared.

Characterization of Nuclear Protein Binding Sites in the Promoter of Keratin K17 Gene

1996 ◽  
Vol 15 (1) ◽  
pp. 65-74 ◽  
Author(s):  
VLADANA MILISAVLJEVIC ◽  
IRWIN M. FREEDBERG ◽  
MIROSLAV BLUMENBERG
Keyword(s):  
Protein Binding ◽  
Binding Sites ◽  
Nuclear Protein ◽  
Protein Binding Sites
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