scholarly journals Persistent Activation by and Receptor Reserve for an Irreversible A1-Adenosine Receptor Agonist in DDT1 MF-2 Cells and in Guinea Pig Heart

1997 ◽  
Vol 52 (3) ◽  
pp. 491-498 ◽  
Author(s):  
Jiahui Zhang ◽  
Luiz Belardinelli ◽  
Kenneth A. Jacobson ◽  
Deborah H. Otero ◽  
Stephen P. Baker
Keyword(s):  
Guinea Pig ◽  
Adenosine Receptor ◽  
Receptor Agonist ◽  
A1 Adenosine Receptor ◽  
Guinea Pig Heart ◽  
Receptor Reserve ◽  
Adenosine Receptor Agonist

Evidence for regulated coupling of A1 adenosine receptors by phosphorylation in Zucker rats

1995 ◽  
Vol 268 (4) ◽  
pp. E693-E704 ◽  
Author(s):  
D. A. Berkich ◽  
D. R. Luthin ◽  
R. L. Woodard ◽  
S. J. Vannucci ◽  
J. Linden ◽  
...  
Keyword(s):  
Adenosine Receptor ◽  
Adenosine Receptors ◽  
Receptor Agonist ◽  
High Sensitivity ◽  
Zucker Rats ◽  
Guanine Nucleotide ◽  
Agonist Binding ◽  
A1 Adenosine Receptor ◽  
Adenosine Receptor Agonist ◽  
A1 Adenosine Receptors

Studies were designed to find the molecular basis for previous observations that lipolysis is less active and A1 adenosine receptor signaling is more active in adipocytes from obese than from lean Zucker rats. With quantitative immunoblot procedures for detection, Gi alpha 1 and Gs alpha 45 levels were found anomalously low in obese compared with lean membranes (50 and 30%, respectively), but other G alpha subunit levels were normal. However, the sensitivity of the receptor-Gi protein to GTP was about 5- to 10-fold higher in obese compared with lean membranes when assessed from 1) the ability of GTP to inhibit forskolin-stimulated adenylyl cyclase in the presence of an adenosine receptor agonist and 2) the ability of a nonhydrolyzable guanine nucleotide analogue to alter A1 adenosine receptor agonist binding. Alkaline phosphatase treatment of isolated adipocyte membranes from obese but not lean animals decreased guanine nucleotide sensitivity of agonist binding. Surprisingly, solubilized adipocyte A1 adenosine receptors from all animals exhibited the same high sensitivity to guanine nucleotides as that of intact obese membranes, and this high sensitivity could be decreased 20-fold by treatment with alkaline phosphatase. These data suggest that protein phosphorylation may regulate coupling of the A1 adenosine receptor in rat adipocyte membranes.

scholarly journals Circannual Rhythm in Body Temperature, Torpor, and Sensitivity to A1 Adenosine Receptor Agonist in Arctic Ground Squirrels

2013 ◽  
Vol 28 (3) ◽  
pp. 201-207 ◽  
Author(s):  
Jasmine M. Olson ◽  
Tulasi R. Jinka ◽  
Lindy K. Larson ◽  
Jeffrey J. Danielson ◽  
Jeanette T. Moore ◽  
...  
Keyword(s):  
Body Temperature ◽  
Adenosine Receptor ◽  
Receptor Agonist ◽  
Ground Squirrels ◽  
Circannual Rhythm ◽  
A1 Adenosine Receptor ◽  
Adenosine Receptor Agonist ◽  
Arctic Ground Squirrels
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