5-Hydroxytryptamine1AReceptor-Mediated Increases in Receptor Expression and Activation of Nuclear Factor-κB in Transfected Chinese Hamster Ovary Cells

1997 ◽  
Vol 52 (2) ◽  
pp. 221-226 ◽  
Author(s):  
Daniel S. Cowen ◽  
Perry B. Molinoff ◽  
David R. Manning
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Nuclear Factor Κb ◽  
Receptor Expression ◽  
Nuclear Factor ◽  
Ovary Cells

Enhanced inducible mGlu1α receptor expression in Chinese hamster ovary cells

2001 ◽  
Vol 77 (6) ◽  
pp. 1664-1667 ◽  
Author(s):  
Mark S. Nash ◽  
Julie V. Selkirk ◽  
Caroline E. Gaymer ◽  
R. A. John Challiss ◽  
Stefan R. Nahorski
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Receptor Expression ◽  
Ovary Cells

scholarly journals Enhanced IgG1 production by overexpression of nuclear factor kappa B inhibitor zeta (NFKBIZ) in Chinese hamster ovary cells

2017 ◽  
Vol 70 (2) ◽  
pp. 675-685 ◽  
Author(s):  
Masayoshi Onitsuka ◽  
Yukie Kinoshita ◽  
Akitoshi Nishizawa ◽  
Tomomi Tsutsui ◽  
Takeshi Omasa
Keyword(s):  
Chinese Hamster Ovary ◽  
Nuclear Factor Kappa B ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Nuclear Factor ◽  
Ovary Cells ◽  
Nuclear Factor Kappa

scholarly journals High-level stable expression of recombinant 5-HT1A 5-hydroxytryptamine receptors in Chinese hamster ovary cells

1992 ◽  
Vol 285 (3) ◽  
pp. 933-938 ◽  
Author(s):  
A Newman-Tancredi ◽  
R Wootton ◽  
P G Strange
Keyword(s):  
Adenylate Cyclase ◽  
Cell Line ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Receptor Gene ◽  
Chinese Hamster ◽  
Receptor Expression ◽  
Ovary Cells ◽  
Recombinant Cell ◽  
High Level

The human 5-hydroxytryptamine 5-HT1A receptor gene was transfected into Chinese hamster ovary cells. A series of recombinant monoclonal cell lines expressing the receptor were isolated and the properties of one cell line that expressed receptors at a high level (2.8 pmol/mg) were studied in detail. In ligand binding assays with the selective 5-HT1A receptor agonist 2-(NN-di[3H]propylamino)-8-hydroxy-1,2,3,4-tetrahydronaphthalene ([3H]8-OH-DPAT) only a single class of saturable high-affinity binding sites was detected, with a pharmacological profile in competition experiments essentially identical to that of the 5-HT1A receptor of bovine hippocampus. [3H]8-OH-DPAT binding to the recombinant cell membranes was inhibited by GTP, showing that the receptors in the transfected cells couple to G-proteins. A series of 5-hydroxytryptamine agonists inhibited forskolin-stimulated adenylate cyclase activity in the cells and, despite the high level of receptor expression, their apparent efficacies were similar to those observed for inhibition of adenylate cyclase in brain. This recombinant cell line provides a complete model system for studying the 5-HT1A receptor and its transmembrane signalling system. The recombinant cells can also be grown in suspension culture for long periods but, whereas 5-HT1A receptor numbers and receptor regulation by guanine nucleotides are maintained in suspension-grown cells, the inhibition of adenylate cyclase by the 5-HT1A receptor is gradually lost.

scholarly journals IκB Is a Substrate for a Selective Pathway of Lysosomal Proteolysis

1998 ◽  
Vol 9 (8) ◽  
pp. 1995-2010 ◽  
Author(s):  
Ana Maria Cuervo ◽  
Wei Hu ◽  
Bing Lim ◽  
J. Fred Dice
Keyword(s):  
Chinese Hamster Ovary ◽  
Half Life ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Degradation Pathway ◽  
Receptor Protein ◽  
Nuclear Factor ◽  
Lysosomal Degradation ◽  
Ovary Cells ◽  
Nuclear Factor Κ B

In lysosomes isolated from rat liver and spleen, a percentage of the intracellular inhibitor of the nuclear factor κ B (IκB) can be detected in the lysosomal matrix where it is rapidly degraded. Levels of IκB are significantly higher in a lysosomal subpopulation that is active in the direct uptake of specific cytosolic proteins. IκB is directly transported into isolated lysosomes in a process that requires binding of IκB to the heat shock protein of 73 kDa (hsc73), the cytosolic molecular chaperone involved in this pathway, and to the lysosomal glycoprotein of 96 kDa (lgp96), the receptor protein in the lysosomal membrane. Other substrates for this degradation pathway competitively inhibit IκB uptake by lysosomes. Ubiquitination and phosphorylation of IκB are not required for its targeting to lysosomes. The lysosomal degradation of IκB is activated under conditions of nutrient deprivation. Thus, the half-life of a long-lived pool of IκB is 4.4 d in serum-supplemented Chinese hamster ovary cells but only 0.9 d in serum-deprived Chinese hamster ovary cells. This increase in IκB degradation can be completely blocked by lysosomal inhibitors. In Chinese hamster ovary cells exhibiting an increased activity of the hsc73-mediated lysosomal degradation pathway due to overexpression of lamp2, the human form of lgp96, the degradation of IκB is increased. There are both short- and long-lived pools of IκB, and it is the long-lived pool that is subjected to the selective lysosomal degradation pathway. In the presence of antioxidants, the half-life of the long-lived pool of IκB is significantly increased. Thus, the production of intracellular reactive oxygen species during serum starvation may be one of the mechanisms mediating IκB degradation in lysosomes. This selective pathway of lysosomal degradation of IκB is physiologically important since prolonged serum deprivation results in an increase in the nuclear activity of nuclear factor κ B. In addition, the response of nuclear factor κ B to several stimuli increases when this lysosomal pathway of proteolysis is activated.

Malignancy-related characteristics of wild type and drug-resistant chinese hamster ovary cells

Pathology ◽  
1993 ◽  
Vol 25 (3) ◽  
pp. 268-276 ◽  
Author(s):  
Wanda B. Mackinnon ◽  
Marlen Dyne ◽  
Rebecca Hancock ◽  
Carolyn E. Mountford ◽  
Adrienne J. Grant ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Drug Resistant ◽  
Wild Type ◽  
Ovary Cells

Review of the current methods of Chinese Hamster Ovary (CHO) cells cultivation for production of therapeutic protein

2020 ◽  
Vol 17 ◽  
Author(s):  
Shazid Md. Sharker ◽  
Md. Atiqur Rahman
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Mass Production ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Therapeutic Protein ◽  
Specific Productivity ◽  
Ovary Cells ◽  
Further Development ◽  
Interesting Area

Most of clinical approved protein-based drugs or under in clinical trial have a profound impact in the treatment of critical diseases. The mammalian eukaryotic cells culture approaches, particularly the CHO (Chinese Hamster Ovary) cells are mainly used in the biopharmaceutical industry for the mass-production of therapeutic protein. Recent advances in CHO cell bioprocessing to yield recombinant proteins and monoclonal antibodies have enabled the expression of quality protein. The developments of cell lines are possible to upgrade specific productivity. As a result, it holds an interesting area for academic as well as industrial researchers around the world. This review will concentrate on the recent progress of the mammalian CHO cells culture technology and the future scope of further development for the mass-production of protein therapeutics.

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