Internal Trafficking and Surface Mobility of a Functionally Intact β2-Adrenergic Receptor-Green Fluorescent Protein Conjugate

1997 ◽  
Vol 51 (2) ◽  
pp. 177-184 ◽  
Author(s):  
Larry S. Barak ◽  
Stephen S. G. Ferguson ◽  
Jie Zhang ◽  
Christopher Martenson ◽  
Tobias Meyer ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Adrenergic Receptor ◽  
Fluorescent Protein ◽  
Surface Mobility ◽  
Protein Conjugate ◽  
Β2 Adrenergic Receptor ◽  
Green Fluorescent

scholarly journals pHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis

2014 ◽  
Vol 207 (3) ◽  
pp. 419-432 ◽  
Author(s):  
Yi Shen ◽  
Morgane Rosendale ◽  
Robert E. Campbell ◽  
David Perrais
Keyword(s):  
Transferrin Receptor ◽  
Adrenergic Receptor ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Ph Sensitive ◽  
Vesicle Formation ◽  
Red Fluorescent Protein ◽  
Endocytic Vesicle ◽  
Β2 Adrenergic Receptor ◽  
Green Fluorescent

Fluorescent proteins with pH-sensitive fluorescence are valuable tools for the imaging of exocytosis and endocytosis. The Aequorea green fluorescent protein mutant superecliptic pHluorin (SEP) is particularly well suited to these applications. Here we describe pHuji, a red fluorescent protein with a pH sensitivity that approaches that of SEP, making it amenable for detection of single exocytosis and endocytosis events. To demonstrate the utility of the pHuji plus SEP pair, we perform simultaneous two-color imaging of clathrin-mediated internalization of both the transferrin receptor and the β2 adrenergic receptor. These experiments reveal that the two receptors are differentially sorted at the time of endocytic vesicle formation.

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