scholarly journals A Novel Nonconsensus Xenobiotic Response Element Capable of Mediating Aryl Hydrocarbon Receptor-Dependent Gene Expression

2011 ◽  
Vol 81 (3) ◽  
pp. 338-347 ◽  
Author(s):  
Gengming Huang ◽  
Cornelis J. Elferink
Keyword(s):  
Gene Expression ◽  
Aryl Hydrocarbon Receptor ◽  
Response Element ◽  
Aryl Hydrocarbon ◽  
Xenobiotic Response Element ◽  
Xenobiotic Response

scholarly journals Aryl Hydrocarbon Receptor Directly Regulates Artemin Gene Expression

2019 ◽  
Vol 39 (20) ◽  
Author(s):  
Tomohiro Edamitsu ◽  
Keiko Taguchi ◽  
Eri H. Kobayashi ◽  
Ryuhei Okuyama ◽  
Masayuki Yamamoto
Keyword(s):  
Gene Expression ◽  
Aryl Hydrocarbon Receptor ◽  
Chromatin Immunoprecipitation ◽  
Active Form ◽  
Aryl Hydrocarbon ◽  
Xenobiotic Response Element ◽  
Chromatin Immunoprecipitation Sequencing ◽  
Complex Regulation ◽  
Xenobiotic Response ◽  
Direct Induction

ABSTRACT Transgenic mice expressing a constitutively active form of the aryl hydrocarbon receptor in keratinocytes (AhR-CA mice) develop severe dermatitis that substantially recapitulates the pathology of human atopic dermatitis. The neurotrophic factor artemin (Artn) is highly expressed in the epidermis of AhR-CA mice and causes hypersensitivity to itch (alloknesis) by elongating nerves into the epidermis. However, whether the Artn gene is regulated directly by AhR or indirectly through complex regulation associated with AhR remains unclear. To this end, we previously conducted chromatin immunoprecipitation-sequencing analyses of the Artn locus and found a xenobiotic response element (XRE) motif located far upstream (52 kb) of the gene. Therefore, in this study, we addressed whether the XRE actually regulates the Artn gene expression by deleting the region containing the motif. We generated two lines of ArtnΔXRE mice. In the mouse epidermis, inducible expression of the Artn gene by the AhR agonist 3-methylcholanthrene was substantially suppressed compared to that in wild-type mice. Importantly, in AhR-CA::ArtnΔXRE mice, Artn expression was significantly suppressed, and alloknesis was improved. These results demonstrate that the Artn gene is indeed regulated by the distal XRE-containing enhancer, and alloknesis in AhR-CA mice is provoked by the AhR-mediated direct induction of the Artn gene.

scholarly journals Xenobiotic response element binding enriched in both nuclear and microsomal fractions of rat cerebellum

2003 ◽  
Vol 85 (1) ◽  
pp. 264-273 ◽  
Author(s):  
Nobuyuki Kuramoto ◽  
Katsuhiro Baba ◽  
Keiko Gion ◽  
Chie Sugiyama ◽  
Hideo Taniura ◽  
...  
Keyword(s):  
Response Element ◽  
Rat Cerebellum ◽  
Xenobiotic Response Element ◽  
Xenobiotic Response

scholarly journals Neuronal activity enhances aryl hydrocarbon receptor-mediated gene expression and dioxin neurotoxicity in cortical neurons

2008 ◽  
Vol 104 (5) ◽  
pp. 1415-1429 ◽  
Author(s):  
Chun-Hua Lin ◽  
Shu-Hui Juan ◽  
Chen Yu Wang ◽  
Yu-Yo Sun ◽  
Chih-Ming Chou ◽  
...  
Keyword(s):  
Gene Expression ◽  
Aryl Hydrocarbon Receptor ◽  
Neuronal Activity ◽  
Cortical Neurons ◽  
Aryl Hydrocarbon

scholarly journals Nascent RNA sequencing defines dynamic aryl hydrocarbon receptor signaling responses to wood smoke particles

2021 ◽  
Author(s):  
Arnav Gupta ◽  
Sarah K. Sasse ◽  
Lynn Sanford ◽  
Margaret A. Gruca ◽  
Robin D. Dowell ◽  
...  
Keyword(s):  
Gene Expression ◽  
Aryl Hydrocarbon Receptor ◽  
Airway Epithelial Cells ◽  
Wood Smoke ◽  
Primary Role ◽  
Airway Epithelial ◽  
Transcriptional Responses ◽  
Wildfire Smoke ◽  
Smoke Particles ◽  
Aryl Hydrocarbon

AbstractTranscriptional responses to wildfire smoke, an increasingly important cause of human morbidity, are poorly understood. Here, using a combination of precision nuclear run-on sequencing (PRO-seq) and the assay for transposase-accessible chromatin using sequencing (ATAC-seq), we identify rapid and dynamic changes in transcription and chromatin structure in Beas-2B airway epithelial cells after exposure to wood smoke particles (WSP). By comparing 30 and 120 minutes of WSP exposure, we defined three distinct temporal patterns of transcriptional induction and chromatin responses to WSP. Whereas transcription of canonical targets of the aryl hydrocarbon receptor (AHR), such as CYP1A1 and AHRR, was robustly increased after 30 minutes of WSP exposure, transcription of these genes and associated enhancers returned to near baseline at 120 minutes. ChIP-qPCR assays and AHR knockdown confirmed a role for AHR in regulating these transcriptional responses, and we applied bioinformatics approaches to identify novel AHR-regulated pathways and targets including the DNA methyltransferase, DNMT3L, and its interacting factor, SPOCD1. Our analysis also defined a role for NFkB as a primary transcriptional effector of WSP-induced changes in gene expression. The kinetics of AHR- and NFkB-regulated responses to WSP were distinguishable based on the timing of both transcriptional responses and chromatin remodeling, with induction of several cytokines implicated in maintaining the NFkB response. In aggregate, our data establish a direct and primary role for AHR in mediating airway epithelial responses to WSP and identify crosstalk between AHR and NFkB signaling in controlling pro-inflammatory gene expression.

Involvement of aryl hydrocarbon receptor nuclear translocator in EGF-induced c-Jun/Sp1-mediated gene expression

2010 ◽  
Vol 67 (20) ◽  
pp. 3523-3533 ◽  
Author(s):  
Wan-Chen Huang ◽  
Shu-Ting Chen ◽  
Wei-Chiao Chang ◽  
Kwang-Yu Chang ◽  
Wen-Chang Chang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Aryl Hydrocarbon Receptor ◽  
Aryl Hydrocarbon
Sign in / Sign up

Export Citation Format

Share Document