scholarly journals Design, Preparation, and Characterization of High-Activity Mutants of Human Butyrylcholinesterase Specific for Detoxification of Cocaine

2010 ◽  
Vol 79 (2) ◽  
pp. 290-297 ◽  
Author(s):  
Liu Xue ◽  
Mei-Chuan Ko ◽  
Min Tong ◽  
Wenchao Yang ◽  
Shurong Hou ◽  
...  
Keyword(s):  
High Activity ◽  
Human Butyrylcholinesterase

scholarly journals Kinetic characterization of high-activity mutants of human butyrylcholinesterase for the cocaine metabolite norcocaine

2013 ◽  
Vol 457 (1) ◽  
pp. 197-206 ◽  
Author(s):  
Max Zhan ◽  
Shurong Hou ◽  
Chang-Guo Zhan ◽  
Fang Zheng
Keyword(s):  
High Activity ◽  
Kinetic Modelling ◽  
Kinetic Characterization ◽  
In Vivo Tests ◽  
Human Butyrylcholinesterase

Catalytic parameters of butyrylcholinesterase and its mutants against norcocaine have been characterized in comparison with those against cocaine, indicating that the mutants can efficiently metabolize norcocaine, in addition to cocaine. Further in vivo tests and kinetic modelling support the indication.

scholarly journals Characterization of a high-activity mutant of human butyrylcholinesterase against (−)-cocaine

2010 ◽  
Vol 187 (1-3) ◽  
pp. 148-152 ◽  
Author(s):  
Wenchao Yang ◽  
Liu Xue ◽  
Lei Fang ◽  
Xi Chen ◽  
Chang-Guo Zhan
Keyword(s):  
High Activity ◽  
Human Butyrylcholinesterase

scholarly journals Identification and characterization of a phospholipase C activity in resident mouse peritoneal macrophages. Inhibition of the enzyme by phenothiazines

1981 ◽  
Vol 197 (2) ◽  
pp. 523-526 ◽  
Author(s):  
Paul D. Wightman ◽  
Mary Ellen Dahlgren ◽  
James C. Hall ◽  
Philip Davies ◽  
Robert J. Bonney
Keyword(s):  
Phospholipase C ◽  
High Activity ◽  
Peritoneal Macrophages ◽  
Ph Optimum ◽  
Reaction Velocity ◽  
Neutral Ph ◽  
Maximum Reaction ◽  
Mouse Peritoneal Macrophages ◽  
Identification And Characterization

Resident mouse peritoneal macrophages contain a phospholipase C of high activity that is specific for phosphatidylinositol. The activity has a neutral pH optimum, is Ca2+-dependent and has a maximum reaction velocity of 525nmol/h per mg of protein. Certain phenothiazines are potent inhibitors of this activity.

Immobilization and Characterization of Tannase and its Haze-removing

2009 ◽  
Vol 15 (6) ◽  
pp. 545-552 ◽  
Author(s):  
Erzheng Su ◽  
Tao Xia ◽  
Liping Gao ◽  
Qianying Dai ◽  
Zhengzhu Zhang
Keyword(s):  
Kinetic Parameter ◽  
High Activity ◽  
Green Tea ◽  
Storage Stability ◽  
Residual Activity ◽  
Black Tea ◽  
Optimum Temperature ◽  
Oolong Tea ◽  
Optimum Ph

Tannase was effectively immobilized on alginate by the method of crosslinking-entrapment-crosslinking with a high activity recovery of 76.6%. The properties of immobilized tannase were investigated. Its optimum temperature was determined to be 35 ° C, decreasing 10 °C compared with that of free enzyme, whereas the optimum pH of 5.0 did not change. The thermal and pH stabilities of immobilized tannase increased to some degree. The kinetic parameter, Km, for immobilized tannase was estimated to be 11.6 × 10-4 mol/L. Fe2+ and Mn2+ could activate the activity of immobilized tannase. The immobilized tannase was also applied to treat the tea beverage to investigate its haze-removing effect. The content of non-estern catechins in green tea, black tea and oolong tea increased by 52.17%, 12.94% and 8.83%, respectively. The content of estern catechins in green tea, oolong tea and black tea decreased by 20.0%, 16.68% and 5.04%, respectively. The anti-sediment effect of green tea infusion treated with immobilized tannase was significantly increased. The storage stability and reusability of the immobilized tannase were improved greatly, with 72.5% activity retention after stored for 42 days and 86.9% residual activity after repeatedly used for 30 times.

Synthesis and Characterization of Novel PEPPSI type Pd–BICAAC Complexes

2022 ◽  
Author(s):  
Márton Nagyházi ◽  
Balázs Almási ◽  
Ádám Lukács ◽  
Attila Bényei ◽  
Tibor Nagy ◽  
...  
Keyword(s):  
High Activity ◽  
Coupling Reaction ◽  
Kinetic Studies ◽  
Elemental Mercury ◽  
Palladium Complexes ◽  
Synthesis And Characterization ◽  
Heck Coupling ◽  
Moisture Resistant

A series of bicyclic alkylamino carbenes (BICAAC) (where N-aryl = dipp, mes, 2,6-dimethyl-4-(dimethylamino)phenyl, 5a-d) and their novel air- and moisture-resistant pyridine (pyridine, 4 dimethylaminopyridine) containing palladium PEPPSI-type Pd(II) complexes (6a-e) were synthetized and characterized. The new palladium complexes have shown high activity in Mizoroki–Heck coupling reaction even at as low as 100 ppm loading (TON up to 10000). Kinetic studies revealed that reactions carried out in the presence of elemental mercury resulted in decrease in activity. It indicates that the coupling reaction may have both molecular and Pd(0)-mediated catalytic paths.

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