Characterization of the Tritium-Labeled Analog of L-threo-β-Benzyloxyaspartate Binding to Glutamate Transporters

2006 ◽  
Vol 71 (1) ◽  
pp. 294-302 ◽  
Author(s):  
Keiko Shimamoto ◽  
Yasuto Otsubo ◽  
Yasushi Shigeri ◽  
Yoshimi Yasuda-Kamatani ◽  
Masamichi Satoh ◽  
...  
Keyword(s):  
Glutamate Transporters

Characterization of Novel L-threo-β-Benzyloxyaspartate Derivatives, Potent Blockers of the Glutamate Transporters

2004 ◽  
Vol 65 (4) ◽  
pp. 1008-1015 ◽  
Author(s):  
Keiko Shimamoto ◽  
Ryuichi Sakai ◽  
Kiyo Takaoka ◽  
Noboru Yumoto ◽  
Terumi Nakajima ◽  
...  
Keyword(s):  
Glutamate Transporters

Molecular characterization of substrate-binding sites in the glutamate transporter family

2001 ◽  
Vol 29 (6) ◽  
pp. 707-710 ◽  
Author(s):  
B. I. Kanner ◽  
M. P. Kavanaugh ◽  
A. Bendahan
Keyword(s):  
Glutamate Transporter ◽  
Glutamate Transporters ◽  
Amino Acid Residues ◽  
Sodium Potassium ◽  
Synaptic Excitation ◽  
Transporter Family ◽  
Carboxyl Terminal ◽  
Substrate Binding Sites

Glutamate transporters are essential for terminating synaptic excitation and for maintaining extracellular glutamate concentrations below neurotoxic levels. These transporters also mediate a thermodynamically uncoupled chloride flux that is activated by two of the molecules that they transport – sodium and glutamate. Five eukaryotic glutamate transporters have been cloned and identified. They exhibit ~ 50% identity and this homology is even greater in the carboxyl terminal half, which is predicted to have an unusual topology. Determination of the topology shows that the carboxyl terminal part of the molecule contains several transmembrane domains that are separated by at least two re-entrant loops. In these structural elements, we have identified several conserved amino acid residues that play crucial roles in the interaction with the transporter substrates sodium, potassium and glutamate.

[3H](2S,4R)-4-Methylglutamate: a novel ligand for the characterization of glutamate transporters

2001 ◽  
Vol 77 (5) ◽  
pp. 1218-1225 ◽  
Author(s):  
Karina Apricó ◽  
Philip M. Beart ◽  
Andrew J. Lawrence ◽  
Duncan Crawford ◽  
Ross D. O'Shea
Keyword(s):  
Glutamate Transporters

scholarly journals Functional and morphological characterization of glutamate transporters in the rat locus coeruleus

2013 ◽  
Vol 169 (8) ◽  
pp. 1781-1794 ◽  
Author(s):  
M C Medrano ◽  
I Gerrikagoitia ◽  
L Martínez-Millán ◽  
A Mendiguren ◽  
J Pineda
Keyword(s):  
Locus Coeruleus ◽  
Glutamate Transporters ◽  
Morphological Characterization

scholarly journals S3.16 Molecular and functional characterization of new pathogenic mutations in mitochondrial ornithine and aspartate/glutamate transporters

2008 ◽  
Vol 1777 ◽  
pp. S28
Author(s):  
Giuseppe Fiermonte ◽  
Eleonora Paradies ◽  
Alessandra Tessa ◽  
Filippo M. Santorelli ◽  
Carlo Dionisi-Vici ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Glutamate Transporters ◽  
Pathogenic Mutations

Synthesis and Characterization of 4-Methoxy-7-nitroindolinyl-d-aspartate, a Caged Compound for Selective Activation of Glutamate Transporters andN-Methyl-d-aspartate Receptors in Brain Tissue†

Biochemistry ◽  
2005 ◽  
Vol 44 (9) ◽  
pp. 3316-3326 ◽  
Author(s):  
Yanhua H. Huang ◽  
Saurabh R. Sinha ◽  
Olesya D. Fedoryak ◽  
Graham C. R. Ellis-Davies ◽  
Dwight E. Bergles
Keyword(s):  
Brain Tissue ◽  
Glutamate Transporters ◽  
Synthesis And Characterization ◽  
Selective Activation

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

Sign in / Sign up

Export Citation Format

Share Document