Novel Mechanisms of G Protein-Dependent Regulation of Endothelial Nitric-Oxide Synthase

2005 ◽  
Vol 69 (3) ◽  
pp. 975-982 ◽  
Author(s):  
Alexandra V. Andreeva ◽  
Rita Vaiskunaite ◽  
Mikhail A. Kutuzov ◽  
Jasmina Profirovic ◽  
Randal A. Skidgel ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
G Protein ◽  
Endothelial Nitric Oxide Synthase ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

scholarly journals Endothelial nitric oxide synthase interactions with G-protein-coupled receptors

1999 ◽  
Vol 343 (2) ◽  
pp. 335 ◽  
Author(s):  
Mario B. MARRERO ◽  
Virginia J. VENEMA ◽  
Hong JU ◽  
Han HE ◽  
Haiying LIANG ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
G Protein ◽  
Endothelial Nitric Oxide Synthase ◽  
G Protein Coupled Receptors ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide ◽  
G Protein Coupled

Effects of increased pulmonary flow on the expression of endothelial nitric oxide synthase and endothelin-1 in the rat

2002 ◽  
Vol 103 (s2002) ◽  
pp. 289S-293S ◽  
Author(s):  
Zen-Kong DAI ◽  
Mian-Shin TAN ◽  
Chee-Yin CHAI ◽  
Ing-Jun CHEN ◽  
Arco Y. JENG ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Pulmonary Artery ◽  
Mrna Expression ◽  
G Protein ◽  
Endothelial Nitric Oxide Synthase ◽  
Aortocaval Shunt ◽  
Pulmonary Flow ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

The purpose of the study was to assess whether increased pulmonary flow and subsequent development of pulmonary vascular remodelling could alter the expression of endothelial nitric oxide synthase (eNOS) and endothelin-1 (ET-1) in the rat lung. Nine 42-day-old Wistar rats underwent abdominal aortocaval shunt to increase pulmonary blood flow for 12 weeks. The shunt resulted in significant medial hypertrophy of pulmonary artery without significant alterations in pulmonary or systemic blood pressure. Using competitive reverse transcription–PCR, significant increases in the preproET-1 mRNA expression and eNOS mRNA expression in the lungs of rats with abdominal aortocaval shunt were detected. Increased eNOS protein in the lung of shunt rats was also found by Western blot analysis. However, the plasma ET-1 concentration in the pulmonary artery (sham: 5±0.7pg/ml; shunt: 6±0.8pg/ml) or the lung ET-1 content (sham: 218±41ng/g protein; shunt: 224±40ng/g protein) was unchanged. There was an elevated immunohistochemical expression of eNOS, but not ET-1, in the pulmonary vascular endothelium in rats with the shunt. These results suggest that eNOS and ET-1 may be involved in remodelling prior to the development of pulmonary hypertension.

Localization of endothelial nitric oxide synthase in the normal and failing human atrial myocardium

1996 ◽  
Vol 54 ◽  
pp. 786-787
Author(s):  
Chi-Ming Wei ◽  
Margarita Bracamonte ◽  
Shi-Wen Jiang ◽  
Richard C. Daly ◽  
Christopher G.A. McGregor ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Heart Failure ◽  
Nitric Oxide Synthase ◽  
Endothelial Nitric Oxide Synthase ◽  
Cardiac Tissues ◽  
Mammalian Tissues ◽  
End Stage Heart Failure ◽  
End Stage ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

Nitric oxide (NO) is a potent endothelium-derived relaxing factor which also may modulate cardiomyocyte inotropism and growth via increasing cGMP. While endothelial nitric oxide synthase (eNOS) isoforms have been detected in non-human mammalian tissues, expression and localization of eNOS in the normal and failing human myocardium are poorly defined. Therefore, the present study was designed to investigate eNOS in human cardiac tissues in the presence and absence of congestive heart failure (CHF).Normal and failing atrial tissue were obtained from six cardiac donors and six end-stage heart failure patients undergoing primary cardiac transplantation. ENOS protein expression and localization was investigated utilizing Western blot analysis and immunohistochemical staining with the polyclonal rabbit antibody to eNOS (Transduction Laboratories, Lexington, Kentucky).

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