Insulin-Like Growth Factor Binding Protein-2: Contributions of the C-Terminal Domain to Insulin-Like Growth Factor-1 Binding

2005 ◽  
Vol 69 (3) ◽  
pp. 833-845 ◽  
Author(s):  
Megan M. Kibbey ◽  
Mark J. Jameson ◽  
Erin M. Eaton ◽  
Steven A. Rosenzweig
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Factor Binding ◽  
Terminal Domain

Insulin-like growth factor binding protein-2: NMR analysis and structural characterization of the N-terminal domain

Biochimie ◽  
2012 ◽  
Vol 94 (3) ◽  
pp. 608-616 ◽  
Author(s):  
Charles A. Galea ◽  
Mehdi Mobli ◽  
Kerrie A. McNeil ◽  
Terrence D. Mulhern ◽  
John C. Wallace ◽  
...  
Keyword(s):  
Growth Factor ◽  
Structural Characterization ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Nmr Analysis ◽  
Factor Binding ◽  
Terminal Domain

scholarly journals Ligand-binding characteristics of recombinant amino- and carboxyl-terminal fragments of human insulin-like growth factor-binding protein-3

2001 ◽  
Vol 169 (1) ◽  
pp. 123-133 ◽  
Author(s):  
M Galanis ◽  
SM Firth ◽  
J Bond ◽  
A Nathanielsz ◽  
AA Kortt ◽  
...  
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Factor Binding ◽  
Amino Terminal ◽  
Terminal Domain ◽  
Igf I ◽  
Igf Binding ◽  
Carboxyl Terminal ◽  
Igfbp 3

Insulin-like growth factor-binding protein-3 (IGFBP-3) is a member of a family of structurally conserved proteins (IGFBP-1 to -6) which act as carriers and regulators of the mitogenic peptide hormones IGF-I and IGF-II. Members of the IGFBP family share conserved cysteine-rich amino- and carboxyl-terminal regions. The amino-terminal domain of these proteins is recognised to contain an IGF-binding determinant, but evidence to support a binding site in the carboxyl-terminal region of the protein is less rigorous. To further investigate this, we have synthesised both the amino-terminal (residues 1-88; N-88) and carboxyl-terminal (residues 165-264; C-165) domains of human IGFBP-3 in bacteria, as fusion proteins with a carboxyl-terminal FLAG peptide. Although only C-165 showed binding to IGF-I and -II by solution-binding assays, both N-88 and C-165 demonstrated binding to IGF-I and -II by biosensor analysis albeit with reduced affinities compared with full-length IGFBP-3. Only the carboxyl-terminal fragment (C-165) was able to form hetero-trimeric complexes with IGF-I and the acid-labile subunit (ALS). We conclude that the carboxyl-terminal domain of IGFBP-3 contains an IGF-binding determinant and can form ternary complexes with ALS.

Structure, Dynamics and Heparin Binding of the C-terminal Domain of Insulin-like Growth Factor-binding Protein-2 (IGFBP-2)

2006 ◽  
Vol 364 (4) ◽  
pp. 690-704 ◽  
Author(s):  
Zhihe Kuang ◽  
Shenggen Yao ◽  
David W. Keizer ◽  
Chunxiao C. Wang ◽  
Leon A. Bach ◽  
...  
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Heparin Binding ◽  
Factor Binding ◽  
Structure Dynamics ◽  
Terminal Domain

OR04-3 C-TERMINAL DOMAIN OF INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN-2 (IGFBP-2): STRUCTURE, DYNAMICS AND INTERACTION WITH IGFS

2006 ◽  
Vol 16 ◽  
pp. S7
Author(s):  
Briony E Forbes ◽  
Zhihe Kuang ◽  
Shenggen Yao ◽  
David W Keizer ◽  
Chunxiao C Wang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Factor Binding ◽  
Structure Dynamics ◽  
Terminal Domain

scholarly journals Structure and Properties of the C-terminal Domain of Insulin-like Growth Factor-binding Protein-1 Isolated from Human Amniotic Fluid

2005 ◽  
Vol 280 (33) ◽  
pp. 29812-29819 ◽  
Author(s):  
Alberto Sala ◽  
Stefano Capaldi ◽  
Monica Campagnoli ◽  
Beniamino Faggion ◽  
Sara Labò ◽  
...  
Keyword(s):  
Growth Factor ◽  
Amniotic Fluid ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Structure And Properties ◽  
Human Amniotic Fluid ◽  
Factor Binding ◽  
Terminal Domain

Regulation of insulin-like growth factor-binding protein-1 in rat serum

Diabetes ◽  
1994 ◽  
Vol 43 (2) ◽  
pp. 232-239 ◽  
Author(s):  
M. S. Lewitt ◽  
H. Saunders ◽  
J. L. Phyual ◽  
R. C. Baxter
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Rat Serum ◽  
Factor Binding
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