scholarly journals Usefulness of human jejunal spheroid-derived differentiated intestinal epithelial cells for the prediction of intestinal drug absorption in humans

2022 ◽  
pp. DMD-AR-2021-000796
Author(s):  
Kazuyoshi Michiba ◽  
Kazuya Maeda ◽  
Osamu Shimomura ◽  
Yoshihiro Miyazaki ◽  
Shinji Hashimoto ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Drug Absorption ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Intestinal Drug Absorption

Application of human IPSC-derived intestinal epithelial cells for evaluating oral drug absorption

2017 ◽  
Vol 32 (1) ◽  
pp. S70-S71
Author(s):  
Takanori Akazawa ◽  
Shinpei Yoshida ◽  
Koji Takahashi ◽  
Naoki Kusu ◽  
Yoshitaka Yamaguchi ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Drug Absorption ◽  
Intestinal Epithelial Cells ◽  
Oral Drug ◽  
Intestinal Epithelial ◽  
Oral Drug Absorption ◽  
Human Ipsc

scholarly journals Gellan Gum Promotes the Differentiation of Enterocytes from Human Induced Pluripotent Stem Cells

Pharmaceutics ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 951
Author(s):  
Shimeng Qiu ◽  
Tomoki Kabeya ◽  
Isamu Ogawa ◽  
Shiho Anno ◽  
Hisato Hayashi ◽  
...  
Keyword(s):  
Small Intestine ◽  
Epithelial Cells ◽  
Drug Absorption ◽  
Intestinal Epithelial Cells ◽  
Critical Role ◽  
Gellan Gum ◽  
Peptide Transporter ◽  
Control Group ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells

The evaluation of drug pharmacokinetics in the small intestine is critical for developing orally administered drugs. Caucasian colon adenocarcinoma (Caco-2) cells are employed to evaluate drug absorption in preclinical trials of drug development. However, the pharmacokinetic characteristics of Caco-2 cells are different from those of the normal human small intestine. Besides this, it is almost impossible to obtain primary human intestinal epithelial cells of the same batch. Therefore, human iPS cell-derived enterocytes (hiPSEs) with pharmacokinetic functions similar to human intestinal epithelial cells are expected to be useful for the evaluation of drug absorption. Previous studies have been limited to the use of cytokines and small molecules to generate hiPSEs. Dietary fibers play a critical role in maintaining intestinal physiology. We used gellan gum (GG), a soluble dietary fiber, to optimize hiPSE differentiation. hiPSEs cocultured with GG had significantly higher expression of small intestine- and pharmacokinetics-related genes and proteins. The activities of drug-metabolizing enzymes, such as cytochrome P450 2C19, and peptide transporter 1 were significantly increased in the GG treatment group compared to the control group. At the end point of differentiation, the percentage of senescent cells increased. Therefore, GG could improve the differentiation efficiency of human iPS cells to enterocytes and increase intestinal maturation by extending the life span of hiPSEs.

Three-dimensional organization and functional relationships of endocytotic compartments in pig intestinal epithelial cells

1992 ◽  
Vol 50 (1) ◽  
pp. 576-577
Author(s):  
Julian P. Heath ◽  
Buford L. Nichols ◽  
László G. Kömüves
Keyword(s):  
Electron Microscopy ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Three Dimensional ◽  
Intestinal Epithelial ◽  
Cell Surfaces ◽  
Basal Surface ◽  
Functional Relationships

The newborn pig intestine is adapted for the rapid and efficient absorption of nutrients from colostrum. In enterocytes, colostral proteins are taken up into an apical endocytotic complex of channels that transports them to target organelles or to the basal surface for release into the circulation. The apical endocytotic complex of tubules and vesicles clearly is a major intersection in the routes taken by vesicles trafficking to and from the Golgi, lysosomes, and the apical and basolateral cell surfaces.Jejunal tissues were taken from piglets suckled for up to 6 hours and prepared for electron microscopy and immunocytochemistry as previously described.

Thrombin enhances migration of intestinal epithelial cells

2001 ◽  
Vol 120 (5) ◽  
pp. A504-A504
Author(s):  
A NEUMANN ◽  
M DEPKAPRONDZINSKI ◽  
C WILHELM ◽  
K FELGENHAUER ◽  
T CASPRITZ ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial
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