scholarly journals Antisense Oligonucleotide In Vitro Protein Binding Determination in Plasma, Brain and Cerebral Spinal Fluid Using Hybridization LC-MS/MS

2021 ◽  
pp. DMD-AR-2021-000751
Author(s):  
Guilherme Guimaraes ◽  
Long Yuan ◽  
Pei Li
Keyword(s):  
Protein Binding ◽  
Antisense Oligonucleotide ◽  
Cerebral Spinal Fluid ◽  
Spinal Fluid ◽  
Vitro Protein

scholarly journals Bilirubin Production and Oxidation in CSF of Patients with Cerebral Vasospasm after Subarachnoid Hemorrhage

2005 ◽  
Vol 25 (8) ◽  
pp. 1070-1077 ◽  
Author(s):  
Gail J Pyne-Geithman ◽  
Chad J Morgan ◽  
Kenneth Wagner ◽  
Elizabeth M Dulaney ◽  
Janice Carrozzella ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Subarachnoid Hemorrhage ◽  
Cerebral Vasospasm ◽  
Cerebral Spinal Fluid ◽  
Spinal Fluid ◽  
Oxidation Products ◽  
Mortality And Morbidity ◽  
Delayed Cerebral Vasospasm ◽  
And Oxidative Stress

Delayed cerebral vasospasm after subarachnoid hemorrhage (SAH) remains a significant cause of mortality and morbidity; however, the etiology is, as yet, unknown, despite intensive research efforts. Research in this laboratory indicates that bilirubin and oxidative stress may be responsible by leading to formation of bilirubin oxidation products (BOXes), so we investigated changes in bilirubin concentration and oxidative stress in vitro, and in cerebral spinal fluid (CSF) from SAH patients. Non-SAH CSF, a source of heme oxygenase I (HO-1), and blood were incubated, and in vitro bilirubin production measured. Cerebrospinal fluid from SAH patients was collected, categorized using stimulation of vascular smooth muscle metabolism in vitro, and information obtained regarding occurrence of vasospasm in the patients. Cerebral spinal fluid was analyzed for hemoglobin, total protein and bilirubin, BOXes, malonyldialdehyde and peroxidized lipids (indicators of an oxidizing environment), and HO-1 concentration. The formation of bilirubin in vitro requires that CSF is present, as well as whole, non-anti-coagulated blood. Bilirubin, BOXes, HO-1, and peroxidized lipid content were significantly higher in CSF from SAH patients with vasospasm, compared with nonvasospasm SAH CSF, and correlated with occurrence of vasospasm. We conclude that vasospasm may be more likely in patients with elevated BOXes. The conditions necessary for the formation of BOXes are indeed present in CSF from SAH patients with vasospasm, but not CSF from SAH patients without vasospasm.

scholarly journals Divergent subcellular locations of HTLV-I tax and Int-6: A contrast between in vitro protein-protein binding and intracellular protein colocalization

1997 ◽  
Vol 4 (5) ◽  
pp. 229-234 ◽  
Author(s):  
Christine Neuveut ◽  
Dong-Yan Jin ◽  
Oliver J. Semmes ◽  
Francesca Diella ◽  
Robert Callahan ◽  
...  
Keyword(s):  
Protein Binding ◽  
Intracellular Protein ◽  
Vitro Protein

In vitro protein-binding characteristics of delavirdine and its N-dealkylated metabolite

1996 ◽  
Vol 32 (2) ◽  
pp. 81-89 ◽  
Author(s):  
Alan J Chaput ◽  
Robin D'Ambrosio ◽  
Gene D Morse
Keyword(s):  
Protein Binding ◽  
Binding Characteristics ◽  
Vitro Protein

In Vitro Protein Binding of Diclofenac Sodium in Plasma and Synovial Fluid

1987 ◽  
Vol 76 (2) ◽  
pp. 105-108 ◽  
Author(s):  
Keith K.H. Chan ◽  
Kunjbala H. Vyas ◽  
Kenneth D. Brandt
Keyword(s):  
Synovial Fluid ◽  
Protein Binding ◽  
Diclofenac Sodium ◽  
Vitro Protein

In vitro protein binding of propafenone in normal and uraemic human sera

1989 ◽  
Vol 36 (5) ◽  
pp. 495-499 ◽  
Author(s):  
G. L. -Y. Chan ◽  
J. E. Axelson ◽  
J. D. E. Price ◽  
K. M. McErlane ◽  
C. R. Kerr
Keyword(s):  
Protein Binding ◽  
Human Sera ◽  
Vitro Protein

In vitro protein binding interaction studies involving cefixime

1988 ◽  
Vol 9 (3) ◽  
pp. 315-320 ◽  
Author(s):  
Meir Bialer ◽  
Wen Hui Wu ◽  
Robert D. Faulkner ◽  
B. Michael Silber ◽  
Avraham Yacobi
Keyword(s):  
Protein Binding ◽  
Binding Interaction ◽  
Interaction Studies ◽  
Vitro Protein
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