Characterization of the In Vitro and In Vivo Metabolism and Disposition and Cytochrome P450 Inhibition/Induction Profile of Saxagliptin in Human

2012 ◽  
Vol 40 (7) ◽  
pp. 1345-1356 ◽  
Author(s):  
Hong Su ◽  
David W. Boulton ◽  
Anthony Barros ◽  
Lifei Wang ◽  
Kai Cao ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
In Vivo Metabolism

In Vitro and in Vivo Metabolism of Lu-AMBA, a GRP-Receptor Binding Compound, and the Synthesis and Characterization of Its Metabolites

2009 ◽  
Vol 20 (6) ◽  
pp. 1171-1178 ◽  
Author(s):  
Karen E. Linder ◽  
Edmund Metcalfe ◽  
Thangavel Arunachalam ◽  
Jianqing Chen ◽  
Stephen M. Eaton ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Synthesis And Characterization ◽  
In Vivo Metabolism ◽  
Grp Receptor

scholarly journals Characterization of in vitro and in vivo metabolism of AG-024322, a novel cyclin-dependent kinase (CDK)

Health ◽  
2009 ◽  
Vol 01 (04) ◽  
pp. 249-262 ◽  
Author(s):  
Wei-Zhu Zhong ◽  
Bojan Lalovic ◽  
Jenny Zhan
Keyword(s):  
Cyclin Dependent Kinase ◽  
In Vivo Metabolism

Localization and characterization of cytochrome P450 in the brain. In vivo and in vitro investigations on phenytoin- and phenobarbital-inducible isoforms

1995 ◽  
Vol 82-83 ◽  
pp. 655-662 ◽  
Author(s):  
Benedikt Volk ◽  
Ralf P. Meyer ◽  
Friederike von Lintig ◽  
Bernd Ibach ◽  
Rolf Knoth
Keyword(s):  
Cytochrome P450 ◽  
The Brain

Rapid, Refined, and Robust Method for Expression, Purification, and Characterization of Recombinant Human Amyloid-beta M1-42

2019 ◽  
Author(s):  
Priya Prakash ◽  
Travis Lantz ◽  
Krupal P. Jethava ◽  
Gaurav Chopra
Keyword(s):  
Amyloid Beta ◽  
Western Blots ◽  
Force Microscopy ◽  
E Coli ◽  
Atomic Force ◽  
Set Up ◽  
Short Time

Amyloid plaques found in the brains of Alzheimer’s disease (AD) patients primarily consists of amyloid beta 1-42 (Ab42). Commercially, Ab42 is synthetized using peptide synthesizers. We describe a robust methodology for expression of recombinant human Ab(M1-42) in Rosetta(DE3)pLysS and BL21(DE3)pLysS competent E. coli with refined and rapid analytical purification techniques. The peptide is isolated and purified from the transformed cells using an optimized set-up for reverse-phase HPLC protocol, using commonly available C18 columns, yielding high amounts of peptide (~15-20 mg per 1 L culture) in a short time. The recombinant Ab(M1-42) forms characteristic aggregates similar to synthetic Ab42 aggregates as verified by western blots and atomic force microscopy to warrant future biological use. Our rapid, refined, and robust technique to purify human Ab(M1-42) can be used to synthesize chemical probes for several downstream in vitro and in vivo assays to facilitate AD research.

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