Estimation of Transporters Involved in the Hepatobiliary Transport of TA-0201CA Using Sandwich-Cultured Rat Hepatocytes from Normal and Multidrug Resistance-Associated Protein 2-Deficient Rats

2010 ◽  
Vol 38 (9) ◽  
pp. 1505-1513 ◽  
Author(s):  
Hajime Fukuda ◽  
Rikiya Ohashi ◽  
Noriko Ohashi ◽  
Hikaru Yabuuchi ◽  
Ikumi Tamai
Keyword(s):  
Multidrug Resistance ◽  
Rat Hepatocytes ◽  
Hepatobiliary Transport ◽  
Cultured Rat Hepatocytes

scholarly journals Statins alter the hepatobiliary transport of unconjugated and conjugated bilirubin in sandwich-cultured rat hepatocytes

2014 ◽  
Vol 28 (6) ◽  
pp. 1136-1143 ◽  
Author(s):  
Mónika Szabó ◽  
Zsuzsa Veres ◽  
Attila Bátai-Konczos ◽  
Orsolya Kékesi ◽  
Emese Kis ◽  
...  
Keyword(s):  
Rat Hepatocytes ◽  
Hepatobiliary Transport ◽  
Cultured Rat Hepatocytes ◽  
Conjugated Bilirubin

scholarly journals Dexamethasone- and osmolarity-dependent expression of the multidrug-resistance protein 2 in cultured rat hepatocytes1

1999 ◽  
Vol 340 (3) ◽  
pp. 585-591 ◽  
Author(s):  
Ralf KUBITZ ◽  
Ulrich WARSKULAT ◽  
Marcus SCHMITT ◽  
Dieter HÄUSSINGER
Keyword(s):  
Multidrug Resistance ◽  
Culture Medium ◽  
Rat Hepatocytes ◽  
Multidrug Resistance Protein ◽  
Short Term ◽  
Multidrug Resistance Protein 2 ◽  
Long Term Effect ◽  
Cultured Rat Hepatocytes ◽  
Resistance Protein

Expression of the conjugate export pump multidrug-resistance protein 2 (MRP2) in liver is regulated by endotoxin and anti-tumour agents. This paper reports on the effects of dexamethasone and osmolarity on MRP2 expression. MRP2 expression was studied at the protein, mRNA, immunocytochemical and functional levels in cultured rat hepatocytes. Protein and mRNA expression of MRP2 in rat hepatocytes 24 and 48 h after isolation were largely dependent on the presence of dexamethasone (100 nmol/l) in the culture medium. MRP2 was localized at the pseudocanalicular membrane and increased expression of MRP2 was accompanied by a widening of the pseudocanaliculi. In presence of dexamethasone, hypo-osmolarity (205 mosmol/l) led to a strong induction of MRP2 mRNA and protein, whereas expression was decreased by hyperosmolarity (405 mosmol/l). Also, a decay of MRP2 protein and mRNA following dexamethasone withdrawal was osmosensitive. Expression of dipeptidylpeptidase IV, another canalicular protein, was unaffected by dexamethasone and osmolarity. It is concluded that glucocorticoids are strong inducers of MRP2 in liver. Besides short-term carrier insertion/retrieval, osmoregulation of MRP2 also involves a long-term effect on MRP2 expression.

Nutrition and somatomedin. XXVI. Molecular regulation of IGF-I by insulin in cultured rat hepatocytes

Diabetes ◽  
1991 ◽  
Vol 40 (11) ◽  
pp. 1525-1530 ◽  
Author(s):  
L. S. Phillips ◽  
S. Goldstein ◽  
C. I. Pao
Keyword(s):  
Rat Hepatocytes ◽  
Molecular Regulation ◽  
Igf I ◽  
Cultured Rat Hepatocytes
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