Active particle tracking microrheology using artificial thermal noise

2022 ◽  
Vol 66 (1) ◽  
pp. 187-195
Author(s):  
Shalaka K. Kale ◽  
Andrew J. Petruska ◽  
Joseph R. Samaniuk
Keyword(s):  
Particle Tracking ◽  
Thermal Noise ◽  
Active Particle ◽  
Particle Tracking Microrheology

Multiple particle tracking

2018 ◽  
Author(s):  
Eric M. Furst ◽  
Todd M. Squires
Keyword(s):  
Brownian Motion ◽  
Best Practices ◽  
Particle Tracking ◽  
Heterogeneous Material ◽  
Quantitative Measurements ◽  
Particle Tracking Microrheology ◽  
Shell Models ◽  
Multiple Particle Tracking ◽  
Microscopy Data ◽  
Multiple Particle

The fundamentals and best practices of multiple particle tracking microrheology are discussed, including methods for producing video microscopy data, analyzing data to obtain mean-squared displacements and displacement correlations, and, critically, the accuracy and errors (static and dynamic) associated with particle tracking. Applications presented include two-point microrheology, methods for characterizing heterogeneous material rheology, and shell models of local (non-continuum) heterogeneity. Particle tracking has a long history. The earliest descriptions of Brownian motion relied on precise observations, and later quantitative measurements, using light microscopy.

scholarly journals Plate reader microrheology

2020 ◽  
Author(s):  
Robert F. Hawkins ◽  
Gregg A. Duncan
Keyword(s):  
Particle Tracking ◽  
Fluorescence Polarization ◽  
Rotational Diffusion ◽  
Polymeric Materials ◽  
Soft Materials ◽  
Clinical Samples ◽  
Clinical Settings ◽  
Particle Tracking Microrheology ◽  
Plate Reader ◽  
Potential Applications

AbstractIn this work, we report the development of a simplified microrheological method that can be used to rapidly study soft materials. This approach uses fluorescence polarization and a plate reader format to measure the rotational diffusion of nanoparticles within a sample of interest. We show that this measurement is sensitive to viscosity-dependent changes in polymeric soft materials and is correlated with particle tracking microrheology, a previously validated measure of microrheology. Using these fluorescence polarization-based measurements, we describe formalism that enables reasonable estimation of viscosity in polymeric materials after accounting for length-scale dependent effects of the polymer environment on the nanoparticle rotational diffusion. The use of a plate reader format allows this approach to be higher throughput, less technically challenging, and more widely accessible than standard macro- and microrheological methods, making it available to non-experts. This approach has potential applications in academic and industry settings where conventional rheological equipment may not be available, as well as in clinical settings to rapidly characterize human clinical samples.

scholarly journals Particle tracking microrheology of purified gastrointestinal mucins

Biopolymers ◽  
2014 ◽  
Vol 101 (4) ◽  
pp. 366-377 ◽  
Author(s):  
Pantelis Georgiades ◽  
Paul D. A. Pudney ◽  
David J. Thornton ◽  
Thomas A. Waigh
Keyword(s):  
Particle Tracking ◽  
Particle Tracking Microrheology

Particle tracking microrheology of cancer cells in living subjects

2020 ◽  
Vol 39 ◽  
pp. 98-109 ◽  
Author(s):  
Pei-Hsun Wu ◽  
Sanjiv Sam Gambhir ◽  
Christopher M. Hale ◽  
Wei-Chiang Chen ◽  
Denis Wirtz ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Particle Tracking ◽  
Particle Tracking Microrheology

Particle Tracking Microrheology of Lyotropic Liquid Crystals

Langmuir ◽  
2011 ◽  
Vol 27 (10) ◽  
pp. 6171-6178 ◽  
Author(s):  
Mohammad Mydul Alam ◽  
Raffaele Mezzenga
Keyword(s):  
Liquid Crystals ◽  
Particle Tracking ◽  
Lyotropic Liquid Crystals ◽  
Particle Tracking Microrheology

scholarly journals Aging in dense suspensions of soft thermosensitive microgel particles studied with particle-tracking microrheology

2010 ◽  
Vol 81 (1) ◽  
Author(s):  
Dirk van den Ende ◽  
Eko H. Purnomo ◽  
Michel H. G. Duits ◽  
Walter Richtering ◽  
Frieder Mugele
Keyword(s):  
Particle Tracking ◽  
Particle Tracking Microrheology ◽  
Dense Suspensions ◽  
Microgel Particles

Intracellular viscoelasticity of HeLa cells during cell division studied by video particle-tracking microrheology

2013 ◽  
Vol 19 (1) ◽  
pp. 011008 ◽  
Author(s):  
Yin-Quan Chen ◽  
Chia-Yu Kuo ◽  
Ming-Tzo Wei ◽  
Kelly Wu ◽  
Pin-Tzu Su ◽  
...  
Keyword(s):  
Cell Division ◽  
Particle Tracking ◽  
Hela Cells ◽  
Particle Tracking Microrheology

scholarly journals Particle-Tracking Microrheology Using Micro-Optical Coherence Tomography

2016 ◽  
Vol 111 (5) ◽  
pp. 1053-1063 ◽  
Author(s):  
Kengyeh K. Chu ◽  
Diana Mojahed ◽  
Courtney M. Fernandez ◽  
Yao Li ◽  
Linbo Liu ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Particle Tracking ◽  
Optical Coherence ◽  
Particle Tracking Microrheology
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