scholarly journals Acoustofluidic-mediated molecular delivery to human T cells with a three-dimensional-printed flow chamber

2021 ◽  
Vol 150 (6) ◽  
pp. 4534-4547
Author(s):  
Connor S. Centner ◽  
John T. Moore ◽  
Mary E. Baxter ◽  
Zachary T. Long ◽  
Jacob M. Miller ◽  
...  
Keyword(s):  
T Cells ◽  
Three Dimensional ◽  
Flow Chamber ◽  
Molecular Delivery ◽  
Human T Cells

Acoustofluidic-mediated molecular delivery to human T cells for improved immunotherapies

2020 ◽  
Vol 148 (4) ◽  
pp. 2801-2801
Author(s):  
Riyakumari K. Patel ◽  
Mariah Priddy ◽  
Emily Murphy ◽  
Bryce Stamp ◽  
Connor Centner ◽  
...  
Keyword(s):  
T Cells ◽  
Molecular Delivery ◽  
Human T Cells

scholarly journals Three-dimensional localization of T-cell receptors in relation to microvilli using a combination of superresolution microscopies

2016 ◽  
Vol 113 (40) ◽  
pp. E5916-E5924 ◽  
Author(s):  
Yunmin Jung ◽  
Inbal Riven ◽  
Sara W. Feigelson ◽  
Elena Kartvelishvily ◽  
Kazuo Tohya ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Receptors ◽  
Three Dimensional ◽  
Spatial Relation ◽  
Protein Distribution ◽  
Cell Receptors ◽  
Human T Cells ◽  
Antigen Presenting

Leukocyte microvilli are flexible projections enriched with adhesion molecules. The role of these cellular projections in the ability of T cells to probe antigen-presenting cells has been elusive. In this study, we probe the spatial relation of microvilli and T-cell receptors (TCRs), the major molecules responsible for antigen recognition on the T-cell membrane. To this end, an effective and robust methodology for mapping membrane protein distribution in relation to the 3D surface structure of cells is introduced, based on two complementary superresolution microscopies. Strikingly, TCRs are found to be highly localized on microvilli, in both peripheral blood human T cells and differentiated effector T cells, and are barely found on the cell body. This is a decisive demonstration that different types of T cells universally localize their TCRs to microvilli, immediately pointing to these surface projections as effective sensors for antigenic moieties. This finding also suggests how previously reported membrane clusters might form, with microvilli serving as anchors for specific T-cell surface molecules.

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