Correlation trends in Naval Surface Warfare Center Panama City Division’s database of simulated and collected target scattering responses focused on automated target recognition

In the research of intelligent sports vision systems, the stability and accuracy of vision system target recognition, the reasonable effectiveness of task assignment, and the advantages and disadvantages of path planning are the key factors for the vision system to successfully perform tasks. Aiming at the problem of target recognition errors caused by uneven brightness and mutations in sports competition, a dynamic template mechanism is proposed. In the target recognition algorithm, the correlation degree of data feature changes is fully considered, and the time control factor is introduced when using SVM for classification,At the same time, this study uses an unsupervised clustering method to design a classification strategy to achieve rapid target discrimination when the environmental brightness changes, which improves the accuracy of recognition. In addition, the Adaboost algorithm is selected as the machine learning method, and the algorithm is optimized from the aspects of fast feature selection and double threshold decision, which effectively improves the training time of the classifier. Finally, for complex human poses and partially occluded human targets, this paper proposes to express the entire human body through multiple parts. The experimental results show that this method can be used to detect sports players with multiple poses and partial occlusions in complex backgrounds and provides an effective technical means for detecting sports competition action characteristics in complex backgrounds.

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Clickable Cellulosic Surfaces for Peptide-Based Bioassays

10.26434/chemrxiv.8109143.v1 ◽

2019 ◽

Author(s):

Maria Teresa Odinolfi ◽

Alessandro Romanato ◽

Greta Bergamaschi ◽

Alessandro Strada ◽

Laura Sola ◽

...

Keyword(s):

Target Recognition ◽

Real Case ◽

Polymeric Coating ◽

Healthy Controls ◽

Nonspecific Adsorption ◽

Cellulosic Materials ◽

Cellulose Substrates

The use of peptides in paper-based analytics is a highly appealing field, yet it suffers from severe limitations. This is mostly due to the loss of effective target recognition properties of this relatively small bioprobes upon nonspecific adsorption onto cellulose substrates. Here, we address this issue by introducing a simple polymer-based strategy to obtain clickable cellulosic surfaces, that we exploited for the chemoselective bioconjugation of peptide bioprobes. Our method largely outperformed standard adsorption-based immobilization strategy in a challenging, real-case immunoassay, namely the diagnostic discrimination of Zika+ individuals from healthy controls. Of note, the clickable polymeric coating not only allows efficient peptides bioconjugation, but it provides favorable anti-fouling properties to the cellulosic support. We envisage our strategy to broaden the repertoire of cellulosic materials manipulation and promote a renewed interest in peptide-based paper bioassays.

Download Full-text

Clickable Cellulosic Surfaces for Peptide-Based Bioassays

10.26434/chemrxiv.8109143 ◽

2019 ◽

Author(s):

Maria Teresa Odinolfi ◽

Alessandro Romanato ◽

Greta Bergamaschi ◽

Alessandro Strada ◽

Laura Sola ◽

...

Keyword(s):

Target Recognition ◽

Real Case ◽

Polymeric Coating ◽

Healthy Controls ◽

Nonspecific Adsorption ◽

Cellulosic Materials ◽

Cellulose Substrates

The use of peptides in paper-based analytics is a highly appealing field, yet it suffers from severe limitations. This is mostly due to the loss of effective target recognition properties of this relatively small bioprobes upon nonspecific adsorption onto cellulose substrates. Here, we address this issue by introducing a simple polymer-based strategy to obtain clickable cellulosic surfaces, that we exploited for the chemoselective bioconjugation of peptide bioprobes. Our method largely outperformed standard adsorption-based immobilization strategy in a challenging, real-case immunoassay, namely the diagnostic discrimination of Zika+ individuals from healthy controls. Of note, the clickable polymeric coating not only allows efficient peptides bioconjugation, but it provides favorable anti-fouling properties to the cellulosic support. We envisage our strategy to broaden the repertoire of cellulosic materials manipulation and promote a renewed interest in peptide-based paper bioassays.

Download Full-text