Ultrasound-mediated drug delivery with real-time cell permeability measurements

2013 ◽  
Vol 133 (5) ◽  
pp. 3496-3496
Author(s):  
Pavlos Anastasiadis ◽  
Michelle L. Matter ◽  
John S. Allen
Keyword(s):  
Drug Delivery ◽  
Real Time ◽  
Cell Permeability

Ultrasound-mediated drug delivery with real-time cell permeability measurements

2013 ◽  
Author(s):  
Pavlos Anastasiadis ◽  
Michelle L. Matter ◽  
John S. Allen
Keyword(s):  
Drug Delivery ◽  
Real Time ◽  
Cell Permeability

Real-time quantitative monitoring of in vitro nasal drug delivery by a nasal epithelial mucosa-on-a-chip model

2021 ◽  
Author(s):  
Hanieh Gholizadeh ◽  
Hui Xin Ong ◽  
Peta Bradbury ◽  
Agisilaos Kourmatzis ◽  
Daniela Traini ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Real Time ◽  
Nasal Drug Delivery ◽  
Quantitative Monitoring

A Magnetically Guided Self‐Rolled Microrobot for Targeted Drug Delivery, Real‐Time X‐Ray Imaging, and Microrobot Retrieval

2021 ◽  
pp. 2001681
Author(s):  
Kim Tien Nguyen ◽  
Gwangjun Go ◽  
Zhen Jin ◽  
Bobby Aditya Darmawan ◽  
Ami Yoo ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Real Time ◽  
Targeted Drug Delivery ◽  
X Ray ◽  
X Ray Imaging ◽  
Targeted Drug

scholarly journals Interventional MRI-guided catheter placement and real time drug delivery to the central nervous system

2016 ◽  
Vol 16 (6) ◽  
pp. 635-639 ◽  
Author(s):  
Seunggu J. Han ◽  
Krystof Bankiewicz ◽  
Nicholas A. Butowski ◽  
Paul S. Larson ◽  
Manish K. Aghi
Keyword(s):  
Drug Delivery ◽  
Central Nervous System ◽  
Nervous System ◽  
Real Time ◽  
Interventional Mri ◽  
Catheter Placement ◽  
The Central Nervous System ◽  
Mri Guided

scholarly journals NIR-Responsive Lysosomotropic Phototrigger: ʽAIE + ESIPTʼ Active Naphthalene Based Single Component Photoresponsive Nanocarrier with Two-Photon Uncaging and Real-Time Monitoring Ability

2021 ◽  
Author(s):  
Biswajit Roy ◽  
Rakesh Mengji ◽  
Samrat Roy ◽  
Bipul Pal ◽  
Avijit Jana ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Drug Release ◽  
Real Time ◽  
Near Infrared ◽  
Single Component ◽  
Photon Absorption ◽  
Real Time Monitoring ◽  
Two Photon ◽  
Nir Light

In recent times, organelle-targeted drug delivery systems gained tremendous attention due to the site specific delivery of active drug molecules resulting in enhanced bioefficacy. In this context, the phototriggered drug delivery system (DDS) for releasing an active molecule is superior as it provides spatial and temporal control over the release. So far, near infrared (NIR) light responsive organelle targeted DDS has not yet been developed. Hence, we introduced a two-photon NIR-light responsive lysosome targeted ʽAIE + ESIPTʼ active single component DDS based on naphthalene chromophore. The Two-photon absorption cross-section of our DDS is 142 GM at 850 nm. The DDS was converted into pure organic nanoparticles for biological applications. Our nano-DDS is capable of selective targeting, AIE-luminogenic imaging, and drug release within the lysosome. In vitro studies using cancerous cell lines showed that our single component photoresponsive nanocarrier exhibited enhanced cytotoxicity and real-time monitoring ability of the drug release.

Anti-HER2 VHH Targeted Fluorescent Liposome as Bimodal Nanoparticle for Drug Delivery and Optical Imaging

2021 ◽  
Vol 16 ◽  
Author(s):  
Sepideh Khaleghi ◽  
Fatemeh Rahbarizadeh ◽  
Shahryar Khoshtinat Nikkhoi
Keyword(s):  
Drug Delivery ◽  
Real Time ◽  
Targeted Delivery ◽  
Cell Tracking ◽  
Physicochemical Characterization ◽  
Target Cells ◽  
Wide Field ◽  
Cell Trafficking ◽  
Microscopy Imaging ◽  
Intracellular Drug Delivery

Objective: The aim of this study was to formulate fluorescent-labeled targeted immunoliposome to visualize the delivery and distribution of drugs in real-time. Methods: In this study, fluorescent-labeled liposomes were decorated with anti-HER2 VHH or Herceptin to improve the monitoring of intracellular drug delivery and tumor cell tracking with minimal side effects. The conjugation efficiency of antibodies was analyzed by SDS-PAGE silver staining. In addition, the physicochemical characterization of liposomes was performed using DLS and TEM. Finally, confocal microscopy visualized nanoparticles in the target cells. Results: Quantitative and qualitative methods characterized the intracellular uptake of 110±10 nm particles with near 70% conjugation efficiency. In addition, live-cell trafficking during hours of incubation was monitored by wide-field microscopy imaging. The results show that the fluorescent-labeled nanoparticles can specifically bind to HER2-positive breast cancer with minimal off-target delivery. Conclusion: This kind of nanoparticles can have several applications in personalized medicine, especially drug delivery and real-time visualization of cancer therapy. Moreover, this method also can be applied in the targeted delivery of contrast agents in imaging and thermotherapy.

scholarly journals Applications of Nanomaterials for Theranostics of Melanoma

2020 ◽  
Vol 1 (1) ◽  
pp. 39-55
Author(s):  
Guanqiao Jin ◽  
Pohlee Cheah ◽  
Jing Qu ◽  
Lijuan Liu ◽  
Yongfeng Zhao
Keyword(s):  
Drug Delivery ◽  
Molecular Imaging ◽  
Early Diagnosis ◽  
Real Time ◽  
Treatment Plan ◽  
Chemotherapeutic Agents ◽  
The Body ◽  
Diagnostic Methods ◽  
Therapeutic Drug

Melanoma is an aggressive form of skin cancer with a very high mortality rate. Early diagnosis of the disease, the utilization of more potent pharmacological agents, and more effective drug delivery systems are essential to achieve an optimal treatment plan. The applications of nanotechnology to improve therapeutic efficacy and early diagnosis for melanoma treatment have received great interest among researchers and clinicians. In this review, we summarize the recent progress of utilizing various nanomaterials for theranostics of melanoma. The key importance of using nanomaterials for theranostics of melanoma is to improve efficacy and reduce side effects, ensuring safe implementation in clinical use. As opposed to conventional in vitro diagnostic methods, in vivo medical imaging technologies have the advantages of being a type of non-invasive, real-time monitoring. Several common nanoparticles, including ultrasmall superparamagnetic iron oxide nanoparticles, silica nanoparticles, and carbon-based nanoparticles, have been applied to deliver chemotherapeutic agents for the theranostics of melanoma. The application of nanomaterials for theranostics in molecular imaging (MRI, PET, US, OI, etc.) plays an important role in targeting drug delivery of melanoma, by monitoring the distribution site of the molecular imaging probe and the therapeutic drug in the body in real-time. Hence, it is worthwhile to anticipate the approval of these nanomaterials for theranostics in molecular imaging by the US Food and Drug Administration in clinical trials.

Multimodal Cancer Treatment: Real Time Monitoring, Optimization, and Synergistic Effects

2008 ◽  
Vol 7 (5) ◽  
pp. 409-414 ◽  
Author(s):  
Gunnar Myhr
Keyword(s):  
Drug Delivery ◽  
Drug Release ◽  
Adjuvant Therapy ◽  
Ionizing Radiation ◽  
Cancer Treatment ◽  
Real Time ◽  
Treatment System ◽  
Multi Drug Resistance ◽  
Real Time Monitoring ◽  
Site Specific

The primary objective of this analysis is to provide the theoretical framework for a novel multimodal cancer treatment system emphasizing the use of ultrasound as a synergistic drug release mechanism, real time monitoring by MRI of hyperthermic, pO2, and ultrasound induced released effects. The aim is to provide a cure for the 20% of cancer victims who will die of complications from local solid tumors. Adjuvant therapy usually refers to surgery preceding or following chemotherapy and/or ionizing radiation treatment to decrease the risk of recurrence, but the absolute benefit for survival obtained with adjuvant therapy compared to control is only approximately 6%. Tumor hypoxia represents a primary therapeutic concern, besides multi-drug resistance (MDR), because it can reduce the effectiveness of drugs and radiotherapy; well-oxygenated cells require one-third the dose of hypoxic cells to achieve a given level of cell killing. The era of systemic and indiscriminate chemotherapeutic drug delivery into both healthy and pathologic tissues is near an end. Targeted drug delivery using nanoparticles is emerging as the new vehicle, either as a single treatment option, as part of adjuvant procedures or as a component of a multimodal cancer treatment system. There are more than 100 nanosized liposomes or particles, and conjugated anticancer agents in various stages of preclinical and clinical development. Active targeting can be achieved by site-specific delivery or site-specific triggering. Ultrasound can be utilized as both a site triggering and synergistic mechanism in drug release. The process can be monitored using MRI by a physical process called cavitation. An analysis of low frequency ultrasound exposure in combination with liposomally encapsulated doxorubicin (Caelyx) on Balb/c nude mice inoculated with a WiDr (human colon cancer) tumor cell line provided tumor growth inhibition of 30–40%. Mild hyperthermia causes mean intratumor pO2 to increase by 25% and enhances tumor radiosensitization. Hyperthermia causes the extravasation of liposome nanoparticles in deep tumor regions. Ionizing radiation improves the distribution and uptake of drugs. Liposomally encapsulated drugs and ultrasound mediated hyperthermia have been proven to circumvent MDR effects. Hyperthermic effects and pO2 monitoring of bodily fluid have been performed by MRI. It is hypothesized that increased vascularization and subsequent increase in pO2 levels to hypoxic regions, and monitoring of drug release through cavitation, can facilitate optimized real time concomitant or sequential treatments of drug therapy, hyperthermia, ionizing radiation, etc., before or after surgery. An improved therapeutic index with the use of the outlined system seems probable.

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