Sonoporation by single‐shot pulsed ultrasound with microbubbles—Little effect of sonochemical reaction of inertial cavitation

2006 ◽  
Vol 120 (5) ◽  
pp. 3231-3232
Author(s):  
Kengo Okada ◽  
Nobuki Kudo ◽  
Katsuyuki Yamamoto
Keyword(s):  
Single Shot ◽  
Pulsed Ultrasound ◽  
Inertial Cavitation ◽  
Sonochemical Reaction

Inertial cavitation produced by pulsed ultrasound in controlled host media

1996 ◽  
Vol 100 (2) ◽  
pp. 1199-1208 ◽  
Author(s):  
Cheri X. Deng ◽  
Qihong Xu ◽  
Robert E. Apfel ◽  
Christy K. Holland
Keyword(s):  
Pulsed Ultrasound ◽  
Inertial Cavitation

scholarly journals Sonoporation by Single-Shot Pulsed Ultrasound with Microbubbles Adjacent to Cells

2009 ◽  
Vol 96 (12) ◽  
pp. 4866-4876 ◽  
Author(s):  
Nobuki Kudo ◽  
Kengo Okada ◽  
Katsuyuki Yamamoto
Keyword(s):  
Single Shot ◽  
Pulsed Ultrasound

Thresholds for inertial cavitation in Albunex suspensions under pulsed ultrasound conditions

2001 ◽  
Vol 48 (1) ◽  
pp. 161-170 ◽  
Author(s):  
P.P. Chang ◽  
Wen-Shiang Chen ◽  
P.D. Mourad ◽  
S.L. Poliachik ◽  
L.A. Crum
Keyword(s):  
Pulsed Ultrasound ◽  
Inertial Cavitation

Mechanism of Enhancement of Sonochemical-Reaction Efficiency by Pulsed Ultrasound

2008 ◽  
Vol 112 (22) ◽  
pp. 4875-4878 ◽  
Author(s):  
Toru Tuziuti ◽  
Kyuichi Yasui ◽  
Judy Lee ◽  
Teruyuki Kozuka ◽  
Atsuya Towata ◽  
...  
Keyword(s):  
Pulsed Ultrasound ◽  
Reaction Efficiency ◽  
Sonochemical Reaction

scholarly journals Membrane blebbing as a recovery manoeuvre in site-specific sonoporation mediated by targeted microbubbles

2015 ◽  
Vol 12 (105) ◽  
pp. 20150029 ◽  
Author(s):  
Ruen Shan Leow ◽  
Jennifer M. F. Wan ◽  
Alfred C. H. Yu
Keyword(s):  
Pulse Length ◽  
Membrane Dynamics ◽  
Drug Uptake ◽  
Single Shot ◽  
Site Specific ◽  
Membrane Blebbing ◽  
Inertial Cavitation ◽  
New Findings ◽  
Targeted Microbubbles ◽  
Model Drug

Site-specific perforation of the plasma membrane can be achieved through ultrasound-triggered cavitation of a single microbubble positioned adjacent to the cell. However, for this perforation approach (sonoporation), the recovery manoeuvres invoked by the cell are unknown. Here, we report new findings on how membrane blebbing can be a recovery manoeuvre that may take place in sonoporation episodes whose pores are of micrometres in diameter. Each sonoporation site was created using a protocol involving single-shot ultrasound exposure (frequency: 1 MHz; pulse length: 30 cycles; peak negative pressure: 0.45 MPa) which triggered inertial cavitation of a single targeted microbubble (diameter: 1–5 µm). Over this process, live confocal microscopy was conducted in situ to monitor membrane dynamics, model drug uptake kinetics and cytoplasmic calcium ion (Ca 2+ ) distribution. Results show that blebbing would occur at a recovering sonoporation site after its resealing, and it may emerge elsewhere along the membrane periphery. The bleb size was correlated with the pre-exposure microbubble diameter, and 99% of blebbing cases at sonoporation sites were inflicted by microbubbles larger than 1.5 µm diameter (analysed over 124 sonoporation episodes). Blebs were not observed at irreversible sonoporation sites or when sonoporation site repair was inhibited via extracellular Ca 2+ chelation. Functionally, the bleb volume was found to serve as a buffer compartment to accommodate the cytoplasmic Ca 2+ excess brought about by Ca 2+ influx during sonoporation. These findings suggest that membrane blebbing would help sonoporated cells restore homeostasis.

Inertial cavitation dose produced in ex vivo rabbit ear arteries with optison® by 1-mhz pulsed ultrasound

2006 ◽  
Vol 32 (2) ◽  
pp. 281-288 ◽  
Author(s):  
Juan Tu ◽  
Thomas J. Matula ◽  
Andrew A. Brayman ◽  
Lawrence A. Crum
Keyword(s):  
Ex Vivo ◽  
Pulsed Ultrasound ◽  
Inertial Cavitation ◽  
Rabbit Ear

scholarly journals Inertial cavitation initiated by polytetrafluoroethylene nanoparticles under pulsed ultrasound stimulation

2016 ◽  
Vol 32 ◽  
pp. 1-7 ◽  
Author(s):  
Qiaofeng Jin ◽  
Shih-Tsung Kang ◽  
Yuan-Chih Chang ◽  
Hairong Zheng ◽  
Chih-Kuang Yeh
Keyword(s):  
Pulsed Ultrasound ◽  
Inertial Cavitation ◽  
Ultrasound Stimulation
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