Cell lysis using acoustic cavitation bubbles in microfluidics

2012 ◽  
Vol 132 (3) ◽  
pp. 1953-1953 ◽  
Author(s):  
Tandiono Tandiono ◽  
Siew-Wan Ohl ◽  
Cara Sze-Hui Chin ◽  
Dave Siak-Wei Ow ◽  
Claus-Dieter Ohl
Keyword(s):  
Acoustic Cavitation ◽  
Cell Lysis ◽  
Cavitation Bubbles

Theoretical Procedure for the Characterization of Acoustic Cavitation Bubbles

2014 ◽  
Vol 100 (5) ◽  
pp. 823-833 ◽  
Author(s):  
Slimane Merouani ◽  
Oualid Hamdaoui ◽  
Yacine Rezgui ◽  
Miloud Guemini
Keyword(s):  
Acoustic Cavitation ◽  
Cavitation Bubbles ◽  
Theoretical Procedure

scholarly journals APPLICATION OF LASER-INDUCED BREAKDOWN CAVITATION BUBBLES FOR CELL LYSIS IN VITRO

2019 ◽  
pp. 186-190
Author(s):  
DARINA JASIKOVA ◽  
MIROSLAVA RYSOVA ◽  
MICHAL KOTEK
Keyword(s):  
Cavitation Bubble ◽  
Laser Energy ◽  
Cell Lysis ◽  
Basic Mechanism ◽  
Free Cell ◽  
Cavitation Bubbles ◽  
Optical Visualization ◽  
Laser Induced Breakdown ◽  
The Impact

Objective: Understanding the basic mechanism of the cavitation bubble action on living cells as a crucial step of development and application ofsophisticated methods based on controlled cavitation in cell behaviour manipulation. Optimisation of parameters in order to expand cell lysis regioncreated by a single bubble.Methods: The cavitation bubbles are generated by the laser-induced breakdown method. The impact of controlled cavitation bubble on thebiological system is synchronously monitored under a microscope and recorded. Visualization of the cavitation bubble course is monitored by a highspeedcamera. The impact of technology on the healthy confluent cell layer is verified. Evaluation of the cavitation bubbles´ effect on cells in real timeand by subsequent analysis of the cell lysis region and impact of the cavitation bubble on cell viability is carried out by optical visualization and life/dead fluorescence staining.Results: Cavitation bubble induced in distance of 1.5 mm from the cell surface overcomes properties of sessile bubble and enables to create cell lysisregion over 1000 μm in diameter due to transient shear stress produced by liquid displaced by the bubble expansion.Conclusion: Cell lysis region is strongly dependent on the spot laser energy (SLE) and the bubble induction distance from cells. This knowledge iscrucial for application in chemical free cell lysis in vitro, wound induction for experimental purposes and cell layers patterning in desired scale.

scholarly journals Observation of acoustic cavitation bubbles at 2250 frames per second

2001 ◽  
Vol 8 (3) ◽  
pp. 159-162 ◽  
Author(s):  
S. Luther ◽  
R. Mettin ◽  
P. Koch ◽  
W. Lauterborn
Keyword(s):  
Acoustic Cavitation ◽  
Cavitation Bubbles

LITHOTRIPTER SHOCK WAVES CAUSE IN VITRO CELL LYSIS IN THE ABSENCE OF ACOUSTIC CAVITATION

1999 ◽  
pp. 251
Author(s):  
James C. Williams ◽  
Jason Woodward ◽  
Mark A. Stonehill ◽  
Andrew P. Evan ◽  
James A. McAteer
Keyword(s):  
Shock Waves ◽  
Acoustic Cavitation ◽  
Cell Lysis

Correlation between Na* Emission and “Chemically Active” Acoustic Cavitation Bubbles

ChemPhysChem ◽  
2007 ◽  
Vol 8 (16) ◽  
pp. 2331-2335 ◽  
Author(s):  
Devi Sunartio ◽  
Kyuichi Yasui ◽  
Toru Tuziuti ◽  
Teruyuki Kozuka ◽  
Yasuo Iida ◽  
...  
Keyword(s):  
Acoustic Cavitation ◽  
Cavitation Bubbles

Characterization of Acoustic Cavitation Bubbles in Different Sound Fields

2010 ◽  
Vol 114 (34) ◽  
pp. 11010-11016 ◽  
Author(s):  
Adam Brotchie ◽  
Franz Grieser ◽  
Muthupandian Ashokkumar
Keyword(s):  
Acoustic Cavitation ◽  
Sound Fields ◽  
Cavitation Bubbles

The penetration of acoustic cavitation bubbles into micrometer-scale cavities

Ultrasonics ◽  
2016 ◽  
Vol 67 ◽  
pp. 190-198 ◽  
Author(s):  
Haresh Anant Vaidya ◽  
Özgür Ertunç ◽  
Thomas Lichtenegger ◽  
Antonio Delgado ◽  
Andreas Skupin
Keyword(s):  
Acoustic Cavitation ◽  
Cavitation Bubbles ◽  
Micrometer Scale

scholarly journals Particle Removal in Ultrasonic Water Flow Cleaning Role of Cavitation Bubbles as Cleaning Agents

2021 ◽  
Vol 314 ◽  
pp. 218-221
Author(s):  
Keita Ando ◽  
Mao Sugawara ◽  
Riria Sakota
Keyword(s):  
Water Flow ◽  
Acoustic Cavitation ◽  
Silica Particles ◽  
Particle Removal ◽  
Cleaning Performance ◽  
Cleaning Agents ◽  
Cavitation Bubbles ◽  
Visualization Experiments ◽  
Glass Surfaces

Visualization experiments are performed to examine the role of acoustic cavitation bubbles that appear in 0.43-MHz ultrasonic water flow spreading over glass surfaces in the context of physical cleaning. The cleaning performance is evaluated using glass samples on which small silica particles are spin-coated. The visualization suggests that acoustic cavitation bubbles play a major role in particle removal as in the case of conventional cleaning with ultrasonic cleaning baths.

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