Statistical characterization of high‐frequency ultrasonic scattering from human skin in vivo

2000 ◽  
Vol 108 (5) ◽  
pp. 2548-2548
Author(s):  
Balasundar Raju ◽  
Mandayam Srinivasan
Keyword(s):  
Human Skin ◽  
High Frequency ◽  
Ultrasonic Scattering ◽  
Statistical Characterization

In vivo ultrasonic tissue characterization of human skin: Estimation of the slope of the attenuation coefficient

1998 ◽  
Vol 7 ◽  
pp. S46
Author(s):  
C. Guittet ◽  
F. Ossant ◽  
J.P. Remenieras ◽  
M. Berson ◽  
L. Pourcelot
Keyword(s):  
Human Skin ◽  
Attenuation Coefficient ◽  
Tissue Characterization

CHARACTERIZATION OF VITILIGO BY IN VIVO SCATTERING COEFFICIENT OF HUMAN SKIN

2011 ◽  
Vol 04 (01) ◽  
pp. 67-72 ◽  
Author(s):  
WANRONG GAO ◽  
PENG LEE ◽  
XIANLING ZHANG
Keyword(s):  
Optical Coherence Tomography ◽  
Human Skin ◽  
Experimental Results ◽  
Scattering Coefficient ◽  
Optical Coherence ◽  
Scattering Coefficients ◽  
Significant Difference

Scattering coefficients of human skin in vivo with and without vitiligo were measured with optical coherence tomography (OCT). The experimental results show that there exist significant difference between the scattering coefficient of the epidermis of in vivo human skin with and without vitiligo disease. The results may be helpful for quantitatively diagnosing or evaluating the treatment of the disease.

Mechanical Characterization of Human Skin From In-Vivo Tests and Simulation of Reconstructive Surgery

2003 ◽  
Author(s):  
Luigi Gambarotta ◽  
Roberta Massabo ◽  
Renata Morbiducci
Keyword(s):  
Human Skin ◽  
Reconstructive Surgery ◽  
Numerical Procedure ◽  
Experimental Tests ◽  
Element Discretization ◽  
Identification Technique ◽  
Nonlinear Parameter Identification ◽  
In Vivo Characterization

A comprehensive experimental/numerical procedure is presented for the in vivo characterization of the mechanical properties of human skin and the simulation of reconstructive surgery. The procedure uses in vivo experimental tests, which can be performed during surgery, a numerical model formulated within the framework of nonlinear finite strain elasticity and a nonlinear parameter identification technique for the calibration of the model. The skin is treated as a time independent and hyperelastic membrane and the problem is solved through a finite element discretization. The procedure is applied to characterize the scalp skin tested by Raposio and Nordstro¨m, 1998, and to simulate a simple surgical procedure of excision and closure. The application highlights the validity of the proposed approach.

scholarly journals Establishment and Characterization of a Reliable Xenograft Model of Hodgkin Lymphoma Suitable for the Study of Tumor Origin and the Design of New Therapies

Cancers ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 414 ◽  
Author(s):  
Radhia M’kacher ◽  
Monika Frenzel ◽  
Mustafa Al Jawhari ◽  
Steffen Junker ◽  
Corina Cuceu ◽  
...  
Keyword(s):  
Animal Model ◽  
Histone Deacetylase ◽  
High Frequency ◽  
Histone Deacetylase Inhibitor ◽  
Telomerase Activity ◽  
Nsg Mice ◽  
Deacetylase Inhibitor

To identify the cells responsible for the initiation and maintenance of Hodgkin lymphoma (HL) cells, we have characterized a subpopulation of HL cells grown in vitro and in vivo with the aim of establishing a reliable and robust animal model for HL. To validate our model, we challenged the tumor cells in vivo by injecting the alkylating histone-deacetylase inhibitor, EDO-S101, a salvage regimen for HL patients, into xenografted mice. Methodology: Blood lymphocytes from 50 HL patients and seven HL cell lines were used. Immunohistochemistry, flow cytometry, and cytogenetics analyses were performed. The in vitro and in vivo effects of EDO-S101 were assessed. Results: We have successfully determined conditions for in vitro amplification and characterization of the HL L428-c subline, containing a higher proportion of CD30−/CD15− cells than the parental L428 cell line. This subline displayed excellent clonogenic potential and reliable reproducibility upon xenografting into immunodeficient NOD-SCID-gamma (−/−)(NSG) mice. Using cell sorting, we demonstrate that CD30−/CD15− subpopulations can gain the phenotype of the L428-c cell line in vitro. Moreover, the human cells recovered from the seventh week after injection of L428-c cells into NSG mice were small cells characterized by a high frequency of CD30−/CD15− cells. Cytogenetic analysis demonstrated that they were diploid and showed high telomere instability and telomerase activity. Accordingly, chromosomal instability emerged, as shown by the formation of dicentric chromosomes, ring chromosomes, and breakage/fusion/bridge cycles. Similarly, high telomerase activity and telomere instability were detected in circulating lymphocytes from HL patients. The beneficial effect of the histone-deacetylase inhibitor EDO-S101 as an anti-tumor drug validated our animal model. Conclusion: Our HL animal model requires only 103 cells and is characterized by a high survival/toxicity ratio and high reproducibility. Moreover, the cells that engraft in mice are characterized by a high frequency of small CD30−/CD15− cells exhibiting high telomerase activity and telomere dysfunction.

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