Assessment of soft and mineralized tissue formation in a rat bone healing model using quantitative ultrasound (QUS)

2012 ◽  
Vol 131 (4) ◽  
pp. 3458-3458
Author(s):  
Daniel Rohrbach ◽  
Bernhard Hesse ◽  
Bernd Preininger ◽  
Carsten Perka ◽  
Kay Raum
Keyword(s):  
Bone Healing ◽  
Quantitative Ultrasound ◽  
Tissue Formation ◽  
Mineralized Tissue ◽  
Healing Model ◽  
Rat Bone

Podoplanin is dispensable for mineralized tissue formation and maintenance in the Swiss outbred mouse background

genesis ◽  
2021 ◽  
Author(s):  
Masako Toda Nakamura ◽  
Honghao Zhang ◽  
Dayong Guo ◽  
Hiroki Ueharu ◽  
Haichun Pan ◽  
...  
Keyword(s):  
Tissue Formation ◽  
Mineralized Tissue ◽  
Outbred Mouse

scholarly journals Metabolism of rat bone proteoglycans in vivo

1983 ◽  
Vol 216 (3) ◽  
pp. 589-596 ◽  
Author(s):  
C W Prince ◽  
F Rahemtulla ◽  
W T Butler
Keyword(s):  
Extraction Procedure ◽  
Total Radioactivity ◽  
Mineralized Tissue ◽  
Guanidinium Chloride ◽  
Disaccharide Composition ◽  
A Minor ◽  
Papain Digestion ◽  
Rat Bone

Former evaluations of the role of proteoglycans in mineralization have neglected to address the possibility that the metabolism of proteoglycans may be of significance in this regard. This problem was studied by using radiolabeling in vivo of rat calvaria with [35Sulphate for 2-72 h and a sequential extraction procedure to yield two pools of newly synthesized proteoglycans: one obtained from non-mineralized tissue by extraction with guanidinium chloride (GdmCl) and another obtained only after demineralization with EDTA. Total radioactivity in calvaria was maximal after 12 h of incorporation, but by 36 h had declined to a level that was about 55-65% of maximum. Radioactivity in the GdmCl extract declined steadily after 12 h, whereas that in the EDTA extract remained constant until 36 h, when it began to increase. Each extract contained a minor proteoglycan that eluted at the void volume (Vo) of a Sepharose CL-6B column. Unlike in the EDTA extract, this proteoglycan gradually disappeared from the GdmCl extract. Each extract also contained a major, smaller proteoglycan, with a Kav. of 0.24 and 0.36 in the GdmCl and EDTA extracts respectively. Papain digestion of each extract yielded glycosaminoglycan chains with Kav. values of 0.32 and 0.50 on CL-6B in the GdmCl and EDTA extracts respectively. Digestion of each extract with chondroitinase ABC and chondroitinase AC showed that the glycosaminoglycans were of similar disaccharide composition, with about 85% being 4-sulphated and the remainder 6-sulphated and/or iduronic acid-containing. These data suggest that about 45% of the newly synthesized proteoglycans are removed from the tissue during the course of mineralization.

Corrigendum to “A mechano-regulatory bone-healing model incorporating cell-phenotype specific activity”

2008 ◽  
Vol 254 (3) ◽  
pp. 717
Author(s):  
Hanna Isaksson ◽  
Corrinus C. van Donkelaar ◽  
Rik Huiskes ◽  
Keita Ito
Keyword(s):  
Bone Healing ◽  
Specific Activity ◽  
Cell Phenotype ◽  
Healing Model

Manipulations in Hydrogel Degradation Behavior Enhance Osteoblast Function and Mineralized Tissue Formation

2006 ◽  
Vol 0 (0) ◽  
pp. 060706073730047
Author(s):  
Danielle S.W. Benoit ◽  
Andrew R. Durney ◽  
Kristi S. Anseth
Keyword(s):  
Tissue Formation ◽  
Degradation Behavior ◽  
Mineralized Tissue ◽  
Osteoblast Function

Mineralized Tissue Formation Associated With 2 Different Dental Implant Designs: Histomorphometric Analyses Performed in Dogs

2011 ◽  
Vol 37 (3) ◽  
pp. 319-324 ◽  
Author(s):  
Sergio Olate ◽  
Henrique Duque de Miranda Chaves Netto ◽  
Leandro Eduardo Klüppel ◽  
Renato Mazzonetto ◽  
Jose Ricardo de Albergaria-Barbosa
Keyword(s):  
Bone Formation ◽  
Dental Implants ◽  
Clinical Success ◽  
Tissue Formation ◽  
Mineralized Tissue ◽  
Significance Level ◽  
Significant Difference ◽  
Screw Threads ◽  
Cervical Area ◽  
Apical Area

Abstract The clinical success of dental implants might be associated with such factors as installation technique, implant shape, size, material, and screw threads. Therefore, the aim of this study is to analyze mineralized tissue formation on the screw threads of conical and cylindrical dental implants. This study includes 7 beagle dogs that had the lower premolars extracted. Three months after bone and soft tissue repair, 2 different designs of dental implants (1 conical and 1 cylindrical) were installed in each hemimandible using a nonsubmerged technique. Both implants when installed had different shape and thread, as revealed by scanning electron microscopy. Six weeks after implant installation, animals were killed and submitted to histomorphometric analysis. Cervical, middle, and apical areas were analyzed. Statistical analysis was carried out using Student t test at a significance level of P < .05. Statistically significant differences were not found between the conical and cylindrical implants. The conical implants presented fewer threads, a smaller area, and more bone formation when compared with the cylindrical ones, without significant differences (P  =  .1226). The highest values concerning bone formation were observed for the cervical area (P  =  .4005), and the lowest for the apical area (P  =  .1899); however, no statistically significant difference was observed. In conclusion, no statistically significant difference was observed in thread bone formation between the cylindrical and conical implant designs when placed using the nonsubmerged technique.

Analysis of Pulpal Reactions to Restorative Procedures, Materials, Pulp Capping, and Future Therapies

2002 ◽  
Vol 13 (6) ◽  
pp. 509-520 ◽  
Author(s):  
Peter E. Murray ◽  
L. Jack Windsor ◽  
Thomas W. Smyth ◽  
Abeer A. Hafez ◽  
Charles F. Cox
Keyword(s):  
Dental Health ◽  
Management Strategies ◽  
The United States ◽  
Tissue Formation ◽  
Oral Diseases ◽  
Mineralized Tissue ◽  
Operative Complications ◽  
Caries Management ◽  
Caries Lesions ◽  
Post Operative Complications

Every year, despite the effectiveness of preventive dentistry and dental health care, 290 million fillings are placed each year in the United States; two-thirds of these involve the replacement of failed restorations. Improvements in the success of restorative treatments may be possible if caries management strategies, selection of restorative materials, and their proper use to avoid post-operative complications were investigated from a biological perspective. Consequently, this review will examine pulp injury and healing reactions to different restorative variables. The application of tissue engineering approaches to restorative dentistry will require the transplantation, replacement, or regeneration of cells, and/or stimulation of mineralized tissue formation. This might solve major dental problems, by remineralizing caries lesions, vaccinating against caries and oral diseases, and restoring injured or replacing lost teeth. However, until these therapies can be introduced clinically, the avoidance of post-operative complications with conventional therapies requires attention to numerous aspects of treatment highlighted in this review.

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