In vivo imaging of developing mouse embryos using a high-frequency annular-array and chirp-coded excitation.

2009 ◽  
Vol 126 (4) ◽  
pp. 2214
Author(s):  
Jonathan Mamou ◽  
Jeffrey A. Ketterling ◽  
Orlando Aristizábal ◽  
Daniel H. Turnbull
Keyword(s):  
In Vivo Imaging ◽  
High Frequency ◽  
Mouse Embryos ◽  
Coded Excitation ◽  
Annular Array

In vivo volumetric imaging of the mouse embryonic brain using chirp‐coded excitation and a high‐frequency annular‐array.

2010 ◽  
Vol 128 (4) ◽  
pp. 2364-2364
Author(s):  
Jonathan Mamou ◽  
Jeffrey A. Ketterling ◽  
Orlando Aristizábal ◽  
Daniel H. Turnbull
Keyword(s):  
High Frequency ◽  
Embryonic Brain ◽  
Coded Excitation ◽  
Volumetric Imaging ◽  
Annular Array

In vivo imaging of blood flow in the mouse Achilles tendon using high-frequency ultrasound

Ultrasonics ◽  
2009 ◽  
Vol 49 (2) ◽  
pp. 226-230 ◽  
Author(s):  
Chih-Kuang Yeh ◽  
Jia-Jiun Chen ◽  
Meng-Lin Li ◽  
Jer-Junn Luh ◽  
Jia-Jin Jason Chen
Keyword(s):  
Blood Flow ◽  
Achilles Tendon ◽  
In Vivo Imaging ◽  
High Frequency ◽  
High Frequency Ultrasound ◽  
Frequency Ultrasound

scholarly journals Prime editing primarily induces undesired outcomes in mice

2020 ◽  
Author(s):  
Tomomi Aida ◽  
Jonathan J. Wilde ◽  
Lixin Yang ◽  
Yuanyuan Hou ◽  
Mengqi Li ◽  
...  
Keyword(s):  
Genome Editing ◽  
High Frequency ◽  
Mouse Embryos ◽  
Biomedical Science ◽  
New Approach ◽  
Chemically Modified ◽  
Highly Efficient ◽  
Large Deletions

SummaryGenome editing has transformed biomedical science, but is still unpredictable and often induces undesired outcomes. Prime editing (PE) is a promising new approach due to its proposed flexibility and ability to avoid unwanted indels. Here, we show highly efficient PE-mediated genome editing in mammalian zygotes. Utilizing chemically modified guideRNAs, PE efficiently introduced 10 targeted modifications including substitutions, deletions, and insertions across 6 genes in mouse embryos. However, we unexpectedly observed a high frequency of undesired outcomes such as large deletions and found that these occurred more often than pure intended edits across all of the edits/genes. We show that undesired outcomes result from the double-nicking PE3 strategy, but that omission of the second nick largely ablates PE function. However, sequential double-nicking with PE3b, which is only applicable to a fraction of edits, eliminated undesired outcomes. Overall, our findings demonstrate the promising potential of PE for predictable, flexible, and highly efficient in vivo genome editing, but highlight the need for improved variations of PE before it is ready for widespread use.

40 MHz annular-array in utero imaging of mouse embryos with chirp coded excitation

2008 ◽  
Author(s):  
Orlando Arsitizabal ◽  
Daniel H. Turnbull ◽  
Jonathan Mamou ◽  
Jeffrey A. Ketterling
Keyword(s):  
In Utero ◽  
Mouse Embryos ◽  
Coded Excitation ◽  
Annular Array

In‐utero imaging of developing mouse embryos using a 34‐MHz annular array and chirp‐coded excitation.

2009 ◽  
Vol 125 (4) ◽  
pp. 2514-2514
Author(s):  
Jonathan Mamou ◽  
Jeffrey A. Ketterling ◽  
Orlando Aristizábal ◽  
Daniel H. Turnbull
Keyword(s):  
In Utero ◽  
Mouse Embryos ◽  
Coded Excitation ◽  
Annular Array

scholarly journals In Vivo Imaging of Cerebral Hemodynamics Using High-Frequency Micro-Ultrasound

2010 ◽  
Vol 2010 (9) ◽  
pp. pdb.prot5495-pdb.prot5495 ◽  
Author(s):  
J. Sun ◽  
L. Lindvere ◽  
M. E. van Raaij ◽  
A. Dorr ◽  
B. Stefanovic ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
High Frequency ◽  
Cerebral Hemodynamics
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