Poster - 13: Evaluation of an in-house CCD camera film dosimetry imaging system for small field deliveries

2016 ◽  
Vol 43 (8Part2) ◽  
pp. 4938-4938 ◽  
Author(s):  
Michel Lalonde ◽  
Kevin Alexander ◽  
Tim Olding ◽  
L. John Schreiner ◽  
Andrew T. Kerr
Keyword(s):  
Imaging System ◽  
Ccd Camera ◽  
Small Field ◽  
Film Dosimetry

SU-E-T-51: Characterization of a Novel CCD Camera Based Imaging System for Radiochromic Film Dosimetry

2013 ◽  
Vol 40 (6Part11) ◽  
pp. 214-215
Author(s):  
K Alexander ◽  
T Olding ◽  
G Salomons ◽  
LJ Schreiner
Keyword(s):  
Imaging System ◽  
Ccd Camera ◽  
Radiochromic Film ◽  
Film Dosimetry

Multi-mode light microscopy of microtubule assembly dynamics and chromosome movement in vivo and In vitro

1996 ◽  
Vol 54 ◽  
pp. 730-731
Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer
Keyword(s):  
Imaging System ◽  
Ccd Camera ◽  
Transmitted Light ◽  
Microtubule Assembly ◽  
Live Cells ◽  
Optical Efficiency ◽  
Multiple Band ◽  
Multi Mode

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.

Spatio-temporal measurement of beam properties in the PLS diagnostic beamline

1998 ◽  
Vol 5 (3) ◽  
pp. 642-644 ◽  
Author(s):  
J. Y. Huang ◽  
I. S. Ko
Keyword(s):  
Visible Light ◽  
Imaging System ◽  
Ccd Camera ◽  
Photon Beam ◽  
Transverse Beam ◽  
Beam Position ◽  
X Ray ◽  
X Ray Imaging ◽  
Visible Light Imaging ◽  
Temporal Measurement

A diagnostic beamline is being constructed in the PLS storage ring for measurement of electron- and photon-beam properties. It consists of two 1:1 imaging systems: a visible-light imaging system and a soft X-ray imaging system. In the visible-light imaging system, the transverse beam size and beam position are measured with various detectors: a CCD camera, two photodiode arrays and a photon-beam position monitor. Longitudinal bunch structure is also investigated with a fast photodiode detector and a picosecond streak camera. On the other hand, the soft X-ray imaging system is under construction to measure beam sizes with negligible diffraction-limited error. The X-ray image optics consist of a flat cooled mirror and two spherical focusing mirrors.

scholarly journals Using a CCD Camera Imaging System as a Recording Device to Quantify Human DNA by Slot Blot Hybridization

BioTechniques ◽  
2001 ◽  
Vol 30 (3) ◽  
pp. 680-685 ◽  
Author(s):  
Bruce Budowle ◽  
William R. Hudlow ◽  
Steven B. Lee ◽  
Leonard Klevan
Keyword(s):  
Imaging System ◽  
Blot Hybridization ◽  
Ccd Camera ◽  
Recording Device ◽  
Slot Blot ◽  
Camera Imaging ◽  
Human Dna ◽  
Slot Blot Hybridization

Recent Development of Cone-Beam X-Ray Microtomography at Sunyab

1997 ◽  
Vol 3 (S2) ◽  
pp. 1125-1126
Author(s):  
S.J. Pan ◽  
A. Shih ◽  
W.S. Liou ◽  
M.S. Park ◽  
G. Wang ◽  
...  
Keyword(s):  
Dynamic Range ◽  
Imaging System ◽  
Tomographic Reconstruction ◽  
Ccd Camera ◽  
Test Sample ◽  
Glass Beads ◽  
Cone Beam ◽  
Projection Data ◽  
X Ray ◽  
Cooled Ccd

An experimental X-ray cone-beam microtomographic imaging system utilizing a generalized Feldkamp reconstruction algorithm has been developed in our laboratory. This microtomographic imaging system consists of a conventional dental X-ray source (Aztech 65, Boulder, CO), a sample position and rotation stage, an X-ray scintillation phosphor screen, and a high resolution slow scan cooled CCD camera (Kodak KAF 1400). A generalized Feldkamp cone-beam algorithm was used to perform tomographic reconstruction from cone-beam projection data. This algorithm was developed for various hardware configuration to perform reconstruction of spherical, rod-shaped and plate-like specimen.A test sample consists of 8 glass beads (approx. 800μm in diameter) dispersed in an epoxy-filled #0 gelatin capsule. One hundred X-ray projection images were captured equal angularly (at 3.6 degree spacing) by the cooled CCD camera at a of 1317×967 (17×17mm2) pixels with 12-bit dynamic range. Figure 1 shows a 3D isosurface rendering of the test sample. The eight glass beads and trapped air bubbles (arrows) in the epoxy resin (e) are clearly visible.

Multi-Mode Light Microscopy of Microtubule and Endoplasmic Reticulum Dynamics in Migrating Newt Epithelial Cells.

1997 ◽  
Vol 3 (S2) ◽  
pp. 211-212
Author(s):  
C. M. Waterman-Storer ◽  
E. D. Salmon
Keyword(s):  
Digital Imaging ◽  
Imaging System ◽  
Ccd Camera ◽  
Diaphragm Position ◽  
Multiple Band ◽  
Fluorescence Light ◽  
Band Pass ◽  
Cooled Ccd ◽  
System 1 ◽  
Multi Mode

We have developed a multi-mode digital imaging system (1-3) which acquires images with a 12 bit cooled CCD camera. A multiple band pass dichromatic mirror and robotically controlled excitation filter wheels provide rapid wavelength selection for epi-fluorescence with DAPI, fluorescein or GFP and X-rhodamine fluorophores while maintaining image registration on the cooled CCD detector. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. A robotically controlled emission filter wheel in front of the CCD camera inserts an analyzer in the light path for DIC imaging. To maximize fluorescence light intensity, the analyzer is removed and an optical flat of equivalent optical thickness is inserted for fluorescence imaging. A slider is inserted at the field diaphragm position of the fluorescence epi-illuminator to provide in-focus slit and spot targets for 360 nm photoactivation of “caged” fluorophores. The microscope system is robotically controlled and image acquisition and analysis is performed using MetaMorph™ digital imaging software.

Development of Near-Diffraction Limited, Low-Cost and High-Resolution CCD Camera System for Imaging Coulter Orifices

2014 ◽  
Vol 4 (2) ◽  
pp. 54-61
Author(s):  
Sami D. Alaruri
Keyword(s):  
Imaging System ◽  
Low Cost ◽  
Ccd Camera ◽  
Image Plane ◽  
Geometric Optic ◽  
Optical Path Difference ◽  
Path Difference ◽  
Modulation Transfer ◽  
Camera System ◽  
Micro Lens

A low-cost CCD camera system for imaging Coulter orifices ranging in diameter between 20 µm and 2 mm has been developed and tested. The imaging system incorporates a 6X magnifying lens for viewing the required range of Coulter orifices and LEDs (Light emitting diodes) lamp for back illuminating the orifices. Geometric optic calculations using Zemax® for the micro-lens interfaced with the camera suggest that the spot diameter and the MTF spatial frequency at field of view equal to 0 ° and at the image plane are 5.13 µm and 271.6 lines/mm (at contrast= 37.6%), respectively. Images captured with the camera system for 20 µm, 100 µm and 2 mm diameter orifices are provided. Furthermore, a discussion for the camera micro-lens modulation transfer function, spot diagram, root-mean-square wavefront error versus field and optical path difference plots is given.

Nucleate Boiling From Micro-Machined Surfaces

2003 ◽  
Author(s):  
Adrian M. Holland ◽  
Colin P. Garner
Keyword(s):  
Heat Transfer ◽  
High Speed ◽  
Imaging System ◽  
Laser System ◽  
Ccd Camera ◽  
Nucleate Boiling ◽  
Heat Fluxes ◽  
Frame Rate ◽  
High Speed Imaging ◽  
Machined Surfaces

This paper discusses the production and use of laser-machined surfaces that provide enhanced nucleate boiling and heat transfer characteristics. The surface features of heated plates are known to have a significant effect on nucleate boiling heat transfer and bubble growth dynamics. Nucleate boiling starts from discrete bubbles that form on surface imperfections, such as cavities or scratches. The gas or vapours trapped in these imperfections serve as nuclei for the bubbles. After inception, the bubbles grow to a certain size and depart from the surface. In this work, special heated surfaces were manufactured by laser machining cavities into polished aluminium plates. This was accomplished with a Nd:YAG laser system, which allowed drilling of cavities of a known diameter. The size range of cavities was 20 to 250 micrometers. The resulting nucleate pool boiling was analysed using a novel high-speed imaging system comprising an infrared laser and high resolution CCD camera. This system was operated up to a 2 kHz frame rate and digital image processing allowed bubbles to be analysed statistically in terms of departure diameter, departure frequency, growth rate, shape and velocity. Data was obtained for heat fluxes up to 60 kW.m−2. Bubble measurements were obtained working with water at atmospheric pressure. The surface cavity diameters were selected to control the temperature at which vapour bubbles started to grow on the surface. The selected size and spacing of the cavities was also explored to provide optimal heat transfer.

scholarly journals In Situ Image Acquisition and Measurement of Microdroplets Based on Delay Triggering

Micromachines ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 148
Author(s):  
Xuefeng Chang ◽  
Kang Zheng ◽  
Dan Xie ◽  
Xiayun Shu ◽  
Keyu Xu ◽  
...  
Keyword(s):  
Light Source ◽  
Delay Time ◽  
Imaging System ◽  
Image Acquisition ◽  
Ccd Camera ◽  
Time Interval ◽  
Charge Coupled Device ◽  
Drop On Demand ◽  
Relative Standard

An in situ image acquisition apparatus based on delay triggering for visualizing microdroplets formation is described. The imaging system includes a charge-coupled device camera, a motion control card, a driving circuit, a time delay triggering circuit, and a light source. By adjusting the varying trigger delay time which is synchronized with respect to the signal for jetting, the steady sequential images of the droplet flying in free space can be captured real-time by the system. Several image processing steps are taken to measure the diameters and coordinates of the droplets. Also, the jetting speeds can be calculated according to the delay time interval. For glycerin/water (60:40, mass ratio), under the given conditions of the self-made pneumatically diaphragm-driven drop-on-demand inkjet apparatus, the average of diameter and volume are measured as 266.8 μm and 9944 pL, respectively, and the maximum average velocity of the microdroplets is 0.689 m/s. Finally, the imaging system is applied to measure the volume of 200 microsolder balls generated from the inkjet apparatus. The average diameter is 87.96 μm, and the relative standard deviation is 0.83%. The results show good reproducibility. Unlike previous stroboscopic techniques, the present in situ imaging system which is absence of instantaneous high intensity light employs two control signals to stimulate the microdroplet generator and the charge-coupled device (CCD) camera. Hence, the system can avoid the desynchronization problem of signals which control the strobe light-emitting diode (LED) light source and the camera in previous equipment. This technology is a reliable and cost-effective approach for capturing and measuring microdroplets.

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