TH-AB-204-01: Cherenkov- Excited Luminescence Scanned Imaging (CELSI) for High-Resolution, Deep-Tissue, in Vivo Optical Molecular Imaging with Limited Radiation Dose

2015 ◽  
Vol 42 (6) ◽  
pp. 3713-3714
Author(s):  
R Zhang ◽  
A Dsouza ◽  
J Gunn ◽  
T Esipova ◽  
S Vinogradov ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
High Resolution ◽  
Radiation Dose ◽  
Deep Tissue ◽  
Optical Molecular Imaging ◽  
Excited Luminescence

scholarly journals H-Type Dimer Formation of Fluorophores: A Mechanism for Activatable, in Vivo Optical Molecular Imaging

2009 ◽  
Vol 4 (7) ◽  
pp. 535-546 ◽  
Author(s):  
Mikako Ogawa ◽  
Nobuyuki Kosaka ◽  
Peter L. Choyke ◽  
Hisataka Kobayashi
Keyword(s):  
Molecular Imaging ◽  
Dimer Formation ◽  
Optical Molecular Imaging

scholarly journals Ultrabright red AIEgens for two-photon vascular imaging with high resolution and deep penetration

2018 ◽  
Vol 9 (10) ◽  
pp. 2705-2710 ◽  
Author(s):  
Wei Qin ◽  
Pengfei Zhang ◽  
Hui Li ◽  
Jacky W. Y. Lam ◽  
Yuanjing Cai ◽  
...  
Keyword(s):  
High Resolution ◽  
Vascular Imaging ◽  
Tissue Imaging ◽  
Deep Penetration ◽  
High Contrast ◽  
Deep Tissue ◽  
Two Photon ◽  
High Penetration ◽  
Deep Tissue Imaging

A successful strategy for the design of ultrabright red luminogens with aggregation-induced emission (AIE) features is reported. The AIE dots can be utilized as efficient fluorescent probes for in vivo deep-tissue imaging with high penetration depth and high contrast.

scholarly journals Photoacoustic reporter genes for noninvasive molecular imaging and theranostics

2020 ◽  
Vol 13 (03) ◽  
pp. 2030005
Author(s):  
Zhao Lei ◽  
Yun Zeng ◽  
Xiaofen Zhang ◽  
Xiaoyong Wang ◽  
Gang Liu
Keyword(s):  
Molecular Imaging ◽  
Fluorescent Proteins ◽  
Photoacoustic Imaging ◽  
High Sensitivity ◽  
Reporter Genes ◽  
Biological Processes ◽  
Deep Tissue ◽  
Potential Applications ◽  
Improved Performance

Noninvasive molecular imaging makes the observation and comprehensive understanding of complex biological processes possible. Photoacoustic imaging (PAI) is a fast evolving hybrid imaging technology enabling in vivo imaging with high sensitivity and spatial resolution in deep tissue. Among the various probes developed for PAI, genetically encoded reporters attracted increasing attention of researchers, which provide improved performance by acquiring images of a PAI reporter gene’s expression driven by disease-specific enhancers/promoters. Here, we present a brief overview of recent studies about the existing photoacoustic reporter genes (RGs) for noninvasive molecular imaging, such as the pigment enzyme reporters, fluorescent proteins and chromoproteins, photoswitchable proteins, including their properties and potential applications in theranostics. Furthermore, the challenges that PAI RGs face when applied to the clinical studies are also examined.

scholarly journals In Vivo Optical Molecular Imaging of Matrix Metalloproteinase Activity following Celecoxib Therapy for Colorectal Cancer

2012 ◽  
Vol 11 (5) ◽  
pp. 7290.2012.00003 ◽  
Author(s):  
Rahul A. Sheth ◽  
Alexandra Kunin ◽  
Lars Stangenberg ◽  
Mark Sinnamon ◽  
Kenneth E. Hung ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Molecular Imaging ◽  
Matrix Metalloproteinase ◽  
Optical Molecular Imaging ◽  
Metalloproteinase Activity

In vivo Optical Molecular Imaging of Cardiovascular Diseases: Long Road Ahead

2015 ◽  
Vol 3 (2) ◽  
pp. 118-128
Author(s):  
Yingfeng Tu ◽  
Lei Jiang ◽  
Ruiping Zhang ◽  
Baozhong Shen ◽  
Zhen Cheng
Keyword(s):  
Cardiovascular Diseases ◽  
Molecular Imaging ◽  
Optical Molecular Imaging

Abstract 14935: Targeted Optical Molecular Imaging of Atheroma Calcification Using Novel Aldendronate-based Probe

Circulation ◽  
2020 ◽  
Vol 142 (Suppl_3) ◽  
Author(s):  
Dong Oh Kang ◽  
Yong Geun Lim ◽  
Joon Woo Song ◽  
Ye Hee Park ◽  
Hyun Jung Kim ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
High Resolution ◽  
Murine Model ◽  
Calcium Binding ◽  
Laser Scanning ◽  
Near Infrared ◽  
Imaging System ◽  
Whole Body ◽  
Imaging Results

Background/Objectives: Vascular spotty calcification is an actively regulated biological process resulting in plaque vulnerability. We investigated the feasibility of a novel alendronate-based near-infrared fluorescence (NIRF)-emitting probe to specifically target atherosclerotic calcification in a murine model in vivo using our customized high-resolution multichannel intravital molecular imaging system (IVFM). Methods/Results: We have fabricated a calcium-binding NIRF probe by chemically coupling alendronate, a specific targeting ligand, and NIRF-emitting Cy5.5 to the ends of azide-PEG-NHS ester (Al-Cy5.5). Prepared Al-Cy5.5 has high affinity for calcium phosphate-containing bone minerals. In vitro, Al-Cy5.5 specifically binds to RANKL-induced osteogenic-macrophages as compared to macrophages (p<0.01). On whole body fluorescence imaging to assess time-dependent excretion, NIRF signals remained visible up to 48 hrs. Then, in mice with calcified plaque induced by a combination diet of high-cholesterol and warfarin, Al-Cy5.5 (2.5 mg/kg) was intravenously injected. 48 hrs after administration, murine calcified atheroma was assessed using a customized high-resolution multichannel IVFM, which demonstrated highly enhanced NIRF signals in vivo in the calcified areas of murine carotid plaques (p<0.01, Figure). Ex vivo laser scanning fluorescence microscopic and immune-histological findings from the corresponding sister sections well corroborated the in vivo imaging results, which demonstrated the co-localization of NIRF signals with plaque calcifications (von-Kossa stain). Conclusions: Our novel calcification targeted probe, Al-Cy5.5, was able to selectively target atheroma calcification in vivo in a murine model as assessed by optical IVFM. This novel targetable strategy is expected to provide a promising theranostic basis for calcified high-risk plaques by integration with multimodal customized catheter imaging system.

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