SU-D-213-07: Initial Characterization of a Gel Patch Dosimeter for in Vivo Dosimetry

2015 ◽  
Vol 42 (6) ◽  
pp. 3207-3207
Author(s):  
C Matrosic ◽  
W Culberson ◽  
B Rosen ◽  
E Madsen ◽  
G Frank ◽  
...  
Keyword(s):  
In Vivo Dosimetry ◽  
Initial Characterization

Characterization of microMOSFET detectors for in vivo dosimetry in high‐dose‐rate brachytherapy with 192 Ir

2020 ◽  
Vol 47 (5) ◽  
pp. 2242-2253 ◽  
Author(s):  
Samuel Ruiz‐Arrebola ◽  
Rosa Fabregat‐Borrás ◽  
Eduardo Rodríguez ◽  
Manuel Fernández‐Montes ◽  
Mercedes Pérez‐Macho ◽  
...  
Keyword(s):  
Dose Rate ◽  
High Dose Rate ◽  
In Vivo Dosimetry ◽  
High Dose ◽  
High Dose Rate Brachytherapy

SU-E-T-241: Characterization of MOSFET Detectors for in Vivo Dosimetry in Interventional Radiology and for Dose Reconstruction in Case of Accident

2011 ◽  
Vol 38 (6Part14) ◽  
pp. 3542-3542
Author(s):  
C Bassinet ◽  
C Huet ◽  
M Baumann ◽  
C Etard ◽  
J-L Réhel ◽  
...  
Keyword(s):  
Interventional Radiology ◽  
In Vivo Dosimetry ◽  
Dose Reconstruction

Discovery and initial characterization of YloC, a novel endoribonuclease in Bacillus subtilis

RNA ◽  
2021 ◽  
pp. rna.078962.121
Author(s):  
Shakti Ingle ◽  
Shivani Chhabra ◽  
Jiandong Chen ◽  
Michael B. Lazarus ◽  
Xing Luo ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Small Rna ◽  
Protein Extract ◽  
Rna Regulation ◽  
Initial Characterization ◽  
Oligomeric Form ◽  
Gene Knockouts ◽  
Subtilis Genome

Bacillus subtilis genome is predicted to encode numerous ribonucleases, including four 3’ exoribonucleases that have been characterized to some extent. A strain containing gene knockouts of all four known 3’ exoribonucleases is viable, suggesting that one or more additional RNases remain to be discovered. A protein extract from the quadruple RNase mutant strain was fractionated and RNase activity was followed, resulting in identification of an enzyme activity catalyzed by the YloC protein. YloC is an endoribonuclease and is a member of the highly conserved “YicC family” of proteins that is widespread in bacteria. YloC is a metal-dependent enzyme that catalyzes cleavage of single-stranded RNA, preferentially at U residues, and exists in an oligomeric form, most likely a hexamer. As such, YloC shares some characteristics with the SARS-CoV Nsp15 endoribonuclease. While the in vivo function of YloC in B. subtilis is yet to be determined, YloC was found to act similarly to YicC in an Escherichia coli in vivo assay that assesses decay of the small RNA, RyhB. Thus, YloC may play a role in small RNA regulation.

scholarly journals A General RNA-Binding Protein Complex That Includes the Cytoskeleton-associated Protein MAP 1A

1998 ◽  
Vol 9 (7) ◽  
pp. 1695-1708 ◽  
Author(s):  
Christopher DeFranco ◽  
Marina E. Chicurel ◽  
Huntington Potter
Keyword(s):  
Protein Complex ◽  
Rna Binding ◽  
Fundamental Aspect ◽  
Dependent Manner ◽  
Size Dependent ◽  
Protein Map ◽  
Initial Characterization

Association of mRNA with the cytoskeleton represents a fundamental aspect of RNA physiology likely involved in mRNA transport, anchoring, translation, and turnover. We report the initial characterization of a protein complex that binds RNA in a sequence-independent but size-dependent manner in vitro. The complex includes a ∼160-kDa protein that is bound directly to mRNA and that appears to be either identical or highly related to a ∼1600-kDa protein that binds directly to mRNA in vivo. In addition, the microtubule-associated protein, MAP 1A, a cytoskeletal associated protein is a component of this complex. We suggest that the general attachment of mRNA to the cytoskeleton may be mediated, in part, through the formation of this ribonucleoprotein complex.

scholarly journals 3249 Defining the Extracellular Vesicle Content of Interstitial Fluid for Blood-Free Diagnostics; Extraction Methods and Initial Characterization

2019 ◽  
Vol 3 (s1) ◽  
pp. 7-7
Author(s):  
Justin Baca Robert Taylor ◽  
Srinivasa Rao Gadam ◽  
Lauren Perez
Keyword(s):  
Electron Microscopy ◽  
Minimally Invasive ◽  
Intercellular Communication ◽  
Interstitial Fluid ◽  
Human Subjects ◽  
Extracellular Vesicle ◽  
Vis Spectroscopy ◽  
Initial Characterization

OBJECTIVES/SPECIFIC AIMS: Recent advances in microneedle technology have enabled practical, in vivo dermal interstitial fluid (ISF) sampling. These minimally-invasive techniques allow for collection of ISF without damage to adjacent tissues and do not rely on blister formation. Initial reports of extracellular vesicle (EV) isolation from dermal ISF and paired blood samples suggest that EVs may be more abundant in ISF. Analysis of ISF-derived EVs may allow for more detailed study of intercellular communication at the tissue level, particularly in acute inflammatory conditions. The objective of this study is to describe the isolation and initial characterization of interstitial fluid-derived exosomes. METHODS/STUDY POPULATION: We apply electron microscopy, nanoparticle tracking analysis (NTA), immuochemical, and sequencing methods to describe and distinguish the EV content of interstitial fluid. We include apparently healthy adult human subjects with no active skin disease. We also study immunocompetent, CD-hairless rats to demonstrate the generalizability of the methods. RESULTS/ANTICIPATED RESULTS: We successfully isolated EVs from human and rat interstitial fluid using commercially available precipitation methods. The EVs were initially characterized using UV/Vis spectroscopy, electron microscopy, and NTA. While the study is ongoing, initial results suggest that the concentration and size distribution of EVs differs significantly between blood fractions and ISF. Further immunochemical and sequencing characterization is ongoing. DISCUSSION/SIGNIFICANCE OF IMPACT: We present here the initial characterization of EVs isolated from dermal interstitial fluid. This appears to be the first report of EV characterization using ISF collection methods that do not perturb adjacent tissues (such as with blister or microdialysis methods). The present study lays a foundation for further examination of ISF-derived EVs in acute inflammatory disease such as cellulitis or infectious neuritis. This may enable minimally invasive diagnostics and new research tools to understand intercellular communication in living organisms with increased spatial and temporal resolution.

scholarly journals Structure-Guided Discovery of Novel Aminoglycoside Mimetics as Antibacterial Translation Inhibitors

2005 ◽  
Vol 49 (12) ◽  
pp. 4942-4949 ◽  
Author(s):  
Yuefen Zhou ◽  
Vlad E. Gregor ◽  
Zhongxiang Sun ◽  
Benjamin K. Ayida ◽  
Geoffrey C. Winters ◽  
...  
Keyword(s):  
Respiratory Pathogen ◽  
Gram Negative Bacteria ◽  
The Novel ◽  
Guided Discovery ◽  
Initial Characterization ◽  
Low Toxicity ◽  
Translation Inhibitor

ABSTRACT We report the structure-guided discovery, synthesis, and initial characterization of 3,5-diamino-piperidinyl triazines (DAPT), a novel translation inhibitor class that targets bacterial rRNA and exhibits broad-spectrum antibacterial activity. DAPT compounds were designed as structural mimetics of aminoglycoside antibiotics which bind to the bacterial ribosomal decoding site and thereby interfere with translational fidelity. We found that DAPT compounds bind to oligonucleotide models of decoding-site RNA, inhibit translation in vitro, and induce translation misincorporation in vivo, in agreement with a mechanism of action at the ribosomal decoding site. The novel DAPT antibacterials inhibit growth of gram-positive and gram-negative bacteria, including the respiratory pathogen Pseudomonas aeruginosa, and display low toxicity to human cell lines. In a mouse protection model, an advanced DAPT compound demonstrated efficacy against an Escherichia coli infection at a 50% protective dose of 2.4 mg/kg of body weight by single-dose intravenous administration.

Characterization of MOSFET Detectors for In Vivo Dosimetry in Interventional Radiology and for Dose Reconstruction in Case of Overexposure

2013 ◽  
Vol 104 (4) ◽  
pp. 379-384 ◽  
Author(s):  
Céline Bassinet ◽  
Christelle Huet ◽  
Marion Baumann ◽  
Cécile Etard ◽  
Jean-Luc Réhel ◽  
...  
Keyword(s):  
Interventional Radiology ◽  
In Vivo Dosimetry ◽  
Dose Reconstruction

scholarly journals PO-0825: Characterization of a commercial EPID 3d software for in vivo dosimetry

2016 ◽  
Vol 119 ◽  
pp. S390-S391
Author(s):  
M. Esposito ◽  
P. Bastiani ◽  
A. Bruschi ◽  
A. Ghirelli ◽  
S. Pini ◽  
...  
Keyword(s):  
In Vivo Dosimetry ◽  
3D Software
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