scholarly journals Direct two-dimensional reconstruction algorithm for an inverse-geometry CT system

2009 ◽  
Vol 36 (2) ◽  
pp. 394-401 ◽  
Author(s):  
Jongduk Baek ◽  
Norbert J. Pelc
Keyword(s):  
Reconstruction Algorithm ◽  
Two Dimensional ◽  
Dimensional Reconstruction ◽  
Ct System ◽  
Inverse Geometry

scholarly journals Evaluation of the effect of filters on reconstructed image quality from cone beam CT system

2021 ◽  
Vol 11 (1) ◽  
pp. 35-47
Author(s):  
Ngoc Ha Bui ◽  
Tien Hung Bui ◽  
Thuy Duong Tran ◽  
Kim Tuan Tran ◽  
Ngoc Toan Tran
Keyword(s):  
Image Quality ◽  
Cone Beam Ct ◽  
Three Dimensional ◽  
Reconstruction Algorithm ◽  
Reconstructed Image ◽  
Cone Beam ◽  
Spatial Filters ◽  
Dimensional Reconstruction ◽  
Ct System

: 3D Filtered Back Projection (FBP) is a three-dimensional reconstruction algorithm usually used in Cone Beam Computed Tomography (CBCT) system. FBP is one of the most popular algorithms due to its reconstruction is fast while quality of the result is acceptable. It can also handle a more considerable amount of data with same computer performance with other algorithms. However, the quality of a reconstructed image by the FBP algorithm strongly depends on spatial filters and denoising filters applied to projections. In this paper an evaluation of the reconstructed image quality of the CBCT system by using different denoising filters and spatial filters to find out the best filters for the CBCT system is performed. The result shows that, there is a significantly decrease of the noise of projection with the combination of Median and Gaussian filters. The reconstructed image has high resolution with Cosine filter and becomes more sharpen with Hanning filter.

Computer Assisted Image Reconstruction of Oligomer Structure

1976 ◽  
Vol 34 ◽  
pp. 472-473
Author(s):  
A.M. Jones ◽  
A. Max Fiskin
Keyword(s):  
Three Dimensional ◽  
Depth Of Field ◽  
Computer Assisted ◽  
Projection Data ◽  
Two Dimensional ◽  
Single Particles ◽  
Dimensional Reconstruction ◽  
Computer Assisted Image ◽  
The Fourier Transform ◽  
Space Approach

If the tilt of a specimen can be varied either by the strategy of observing identical particles orientated randomly or by use of a eucentric goniometer stage, three dimensional reconstruction procedures are available (l). If the specimens, such as small protein aggregates, lack periodicity, direct space methods compete favorably in ease of implementation with reconstruction by the Fourier (transform) space approach (2). Regardless of method, reconstruction is possible because useful specimen thicknesses are always much less than the depth of field in an electron microscope. Thus electron images record the amount of stain in columns of the object normal to the recording plates. For single particles, practical considerations dictate that the specimen be tilted precisely about a single axis. In so doing a reconstructed image is achieved serially from two-dimensional sections which in turn are generated by a series of back-to-front lines of projection data.

Three-Dimensional Structure of Cloned T3 Connector Protein at 1.6nm Resolution

1990 ◽  
Vol 48 (1) ◽  
pp. 282-283
Author(s):  
José L. Carrascosa ◽  
José M. Valpuesta ◽  
Hisao Fujisawa
Keyword(s):  
Dna Sequences ◽  
Expression Vector ◽  
Three Dimensional ◽  
Deae Cellulose ◽  
Dna Packaging ◽  
Dimensional Structure ◽  
Two Dimensional ◽  
Freezing And Thawing ◽  
Three Dimensional Structure ◽  
Dimensional Reconstruction

The head to tail connector of bacteriophages plays a fundamental role in the assembly of viral heads and DNA packaging. In spite of the absence of sequence homology, the structure of connectors from different viruses (T4, Ø29, T3, P22, etc) share common morphological features, that are most clearly revealed in their three-dimensional structure. We have studied the three-dimensional reconstruction of the connector protein from phage T3 (gp 8) from tilted view of two dimensional crystals obtained from this protein after cloning and purification.DNA sequences including gene 8 from phage T3 were cloned, into Bam Hl-Eco Rl sites down stream of lambda promotor PL, in the expression vector pNT45 under the control of cI857. E R204 (pNT89) cells were incubated at 42°C for 2h, harvested and resuspended in 20 mM Tris HC1 (pH 7.4), 7mM 2 mercaptoethanol, ImM EDTA. The cells were lysed by freezing and thawing in the presence of lysozyme (lmg/ml) and ligthly sonicated. The low speed supernatant was precipitated by ammonium sulfate (60% saturated) and dissolved in the original buffer to be subjected to gel nitration through Sepharose 6B, followed by phosphocellulose colum (Pll) and DEAE cellulose colum (DE52). Purified gp8 appeared at 0.3M NaCl and formed crystals when its concentration increased above 1.5 mg/ml.

Three-Dimensional Reconstruction of Two Forms of the Chaperonin GRO EL

1990 ◽  
Vol 48 (1) ◽  
pp. 258-259
Author(s):  
J.L. Carrascosa ◽  
G. Abella ◽  
S. Marco ◽  
M. Muyal ◽  
J.M. Carazo
Keyword(s):  
Three Dimensional ◽  
The Other ◽  
Two Dimensional ◽  
Series Method ◽  
Three Dimensional Reconstruction ◽  
Structural Components ◽  
E Coli ◽  
Dimensional Reconstruction ◽  
Morphogenetic Factors ◽  
Tilt Series

Chaperonins are a class of proteins characterized by their role as morphogenetic factors. They trantsiently interact with the structural components of certain biological aggregates (viruses, enzymes etc), promoting their correct folding, assembly and, eventually transport. The groEL factor from E. coli is a conspicuous member of the chaperonins, as it promotes the assembly and morphogenesis of bacterial oligomers and/viral structures.We have studied groEL-like factors from two different bacteria:E. coli and B.subtilis. These factors share common morphological features , showing two different views: one is 6-fold, while the other shows 7 morphological units. There is also a correlation between the presence of a dominant 6-fold view and the fact of both bacteria been grown at low temperature (32°C), while the 7-fold is the main view at higher temperatures (42°C). As the two-dimensional projections of groEL were difficult to interprete, we studied their three-dimensional reconstruction by the random conical tilt series method from negatively stained particles.

scholarly journals An Efficient Signal Reconstruction Algorithm for Stepped Frequency MIMO-SAR in the Spotlight and Sliding Spotlight Modes

2014 ◽  
Vol 2014 ◽  
pp. 1-8
Author(s):  
Jiajia Zhang ◽  
Guangcai Sun ◽  
Mengdao Xing ◽  
Zheng Bao ◽  
Fang Zhou
Keyword(s):  
Efficient Algorithm ◽  
Low Cost ◽  
Signal Reconstruction ◽  
Multiple Input Multiple Output ◽  
Reconstruction Algorithm ◽  
Synthetic Aperture ◽  
Two Dimensional ◽  
Multiple Input ◽  
Input Multiple Output ◽  
Stepped Frequency

Multiple-input multiple-output (MIMO) synthetic aperture radar (SAR) using stepped frequency (SF) waveforms enables a high two-dimensional (2D) resolution with wider imaging swath at relatively low cost. However, only the stripmap mode has been discussed for SF MIMO-SAR. This paper presents an efficient algorithm to reconstruct the signal of SF MIMO-SAR in the spotlight and sliding spotlight modes, which includes Doppler ambiguity resolving algorithm based on subaperture division and an improved frequency-domain bandwidth synthesis (FBS) method. Both simulated and constructed data are used to validate the effectiveness of the proposed algorithm.

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