Sci-PM Thurs - 06: Diffusion studies of human brain tissue in-vivo by MRI

2005 ◽  
Vol 32 (7Part1) ◽  
pp. 2409-2409
Author(s):  
M Nezamzadeh ◽  
I Cameron
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
Human Brain Tissue ◽  
Diffusion Studies

scholarly journals TMOD-12. THE ROLE OF INTEGRIN α V AND CD44 IN GBM MIGRATION USING HUMAN ORGANOTYPIC SLICES

2019 ◽  
Vol 21 (Supplement_6) ◽  
pp. vi265-vi265
Author(s):  
Zev Binder ◽  
Sarah Hyun Ji Kim ◽  
Pei-Hsun Wu ◽  
Anjil Giri ◽  
Gary Gallia ◽  
...  
Keyword(s):  
Human Brain ◽  
Cell Motility ◽  
Brain Tissue ◽  
Brain Slices ◽  
Model System ◽  
Model Systems ◽  
In Vivo Model ◽  
Human Brain Tissue

Abstract Current model systems used for GBM research include traditional in vitro cell line-based assays and in vivo animal studies. In vitro model systems offer the advantages of being easy to use, relatively inexpensive, and fast growing. However, these models lack key elements of the pathology they are attempting to model, including the biochemical and biophysical microenvironment and three-dimensional structure inherent to human brain tissue. In vivo model systems address these limitations, but have restrictions of their own. Species differences may result in non-applicable results and animal experiments are often not designed like clinical trials. Evidence of the limitations of current GBM models is found in the disparity between basic research findings and successful new treatments for GBMs in the clinic. Here we present an alternative model system for the study of human GBM cell motility and invasion, which features advantages of both in vitro and in vivo model systems. Using human organotypic brain slices as scaffolding for tumor growth, we explored the dynamic process of GBM cell invasion within human brain tissue. To demonstrate the utility of the model system, we investigated the effects of depletion of integrin α V (ITGAV) and CD44 on GBM cell motility. These two cell-surface proteins have been identified to have key functions in GBM cell motility. However, knockdown of ITGAV had little effect on tumor cell motility in organotypics while CD44 knockdown significantly reduced cell movement. Finally, we compare motility results from cells in human brain slices to those from cells growing on standard Matrigel and in mouse brain organotypics. We found significant differences in motility depending on the substrate in which the cells were moving. Our findings highlight the physiologic characteristics of human brain organotypics and demonstrate the use of real-time imaging in the ex vivo system.

scholarly journals In situ impedance measurements on postmortem porcine brain

2020 ◽  
Author(s):  
Lucas Poßner ◽  
Matthias Laukner ◽  
Florian Wilhelmy ◽  
Dirk Lindner ◽  
Uwe Pliquett ◽  
...  
Keyword(s):  
Experimental Study ◽  
White Matter ◽  
Human Brain ◽  
Brain Tissue ◽  
Human Brain Tissue ◽  
Experimental Conditions ◽  
Impedance Measurements ◽  
Porcine Brain

AbstractThe paper presents an experimental study where the distinctness of grey and white matter of an in situ postmortem porcine brain by impedance measurements is investigated. Experimental conditions that would allow to conduct the same experiment on in vivo human brain tissue are replicated.https://doi.org/10.1515/cdbme-2019-XXXX

scholarly journals Human cerebrospinal fluid induces neuronal excitability changes in resected human neocortical and hippocampal brain slices

2019 ◽  
Author(s):  
Jenny Wickham ◽  
Andrea Corna ◽  
Niklas Schwarz ◽  
Betül Uysal ◽  
Nikolas Layer ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Human Brain ◽  
Brain Tissue ◽  
Ex Vivo ◽  
Brain Slices ◽  
Electrophysiological Recording ◽  
Recording Medium ◽  
Human Brain Tissue ◽  
Human Cerebrospinal Fluid

AbstractHuman cerebrospinal fluid (hCSF) have proven advantageous over conventional medium when culturing both rodent and human brain tissue. Increased excitability and synchronicity, similar to the active state exclusively recorded in vivo, reported in rodent slice and cell-cultures with hCSF as recording medium, indicates properties of the hCSF not matched by the artificial cerebrospinal fluid (aCSF) commonly used for electrophysiological recording. To evaluate the possible importance of using hCSF as electrophysiological recording medium of human brain tissue, we compared the general excitability in ex vivo human brain tissue slice cultures during perfusion with hCSF and aCSF. For measuring the general activity from a majority of neurons within neocortical and hippocampal human ex vivo slices we used a microelectrode array (MEA) recording technique with 252 electrodes covering an area of 3.2 x 3.2 mm2 and a second CMOS-based MEA with 4225 electrodes on a 2 x 2 mm2 area for detailed mapping of action potential waveforms. We found that hCSF increase the number of active neurons and the firing rate of the neurons in the slices as well as increasing the numbers of bursts while leaving the duration of the bursts unchanged. Interestingly, not only an increase in the overall activity in the slices was observed, but a reconfiguration of the network functionality could be detected with specific activation and inactivation of subpopulations of neuronal ensembles. In conclusion, hCSF is an important component to consider for future human tissue studies, especially for experiments designed to mimic the in vivo situation.

Ex vivo and in vivo imaging of human brain tissue with different OCT systems

2019 ◽  
Author(s):  
Paul Strenge ◽  
Birgit Lange ◽  
Christin Grill ◽  
Wolfgang Draxinger ◽  
Veit Danicke ◽  
...  
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
In Vivo Imaging ◽  
Ex Vivo ◽  
Human Brain Tissue

scholarly journals In-vivo measurements of human brain tissue conductivity using focal electrical current injection through intracerebral multicontact electrodes

2016 ◽  
Vol 38 (2) ◽  
pp. 974-986 ◽  
Author(s):  
Laurent Koessler ◽  
Sophie Colnat-Coulbois ◽  
Thierry Cecchin ◽  
Janis Hofmanis ◽  
Jacek P. Dmochowski ◽  
...  
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
Electrical Current ◽  
Current Injection ◽  
Human Brain Tissue ◽  
Tissue Conductivity ◽  
In Vivo Measurements

Measurement of the force required to move a neurosurgical probe through in vivo human brain tissue

1999 ◽  
Vol 46 (7) ◽  
pp. 891-894 ◽  
Author(s):  
M.A. Howard ◽  
B.A. Abkes ◽  
M.C. Ollendieck ◽  
M.D. Noh ◽  
C. Ritter ◽  
...  
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
Human Brain Tissue

scholarly journals In situ impedance measurements on postmortem porcine brain

2020 ◽  
Vol 6 (3) ◽  
pp. 143-146
Author(s):  
Lucas Poßner ◽  
Matthias Laukner ◽  
Florian Wilhelmy ◽  
Dirk Lindner ◽  
Uwe Pliquett ◽  
...  
Keyword(s):  
Experimental Study ◽  
White Matter ◽  
Human Brain ◽  
Brain Tissue ◽  
Human Brain Tissue ◽  
Experimental Conditions ◽  
Impedance Measurements ◽  
Porcine Brain

AbstractThe paper presents an experimental study where the distinctness of grey and white matter of an in situ postmortem porcine brain by impedance measurements is investigated. Experimental conditions that would allow to conduct the same experiment on in vivo human brain tissue are replicated.

Establishing norms for age-related changes in proton T1 of human brain tissue in vivo

1997 ◽  
Vol 15 (10) ◽  
pp. 1133-1143 ◽  
Author(s):  
Seong Cho ◽  
Dana Jones ◽  
Wilburn E. Reddick ◽  
Robert J. Ogg ◽  
R.Grant Steen
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
Human Brain Tissue ◽  
Age Related ◽  
Age Related Changes
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