TIRF Microscopy

2010 ◽  
pp. 105-105
Keyword(s):  
Tirf Microscopy

scholarly journals Insulin Stimulates Membrane Fusion and GLUT4 Accumulation in Clathrin Coats on Adipocyte Plasma Membranes

2007 ◽  
Vol 27 (9) ◽  
pp. 3456-3469 ◽  
Author(s):  
Shaohui Huang ◽  
Larry M. Lifshitz ◽  
Christine Jones ◽  
Karl D. Bellve ◽  
Clive Standley ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Fusion ◽  
Insulin Signaling ◽  
Plasma Membranes ◽  
Glucose Transporter ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Tirf Microscopy ◽  
Adipocyte Plasma Membranes ◽  
Kinetics Of

ABSTRACT Total internal reflection fluorescence (TIRF) microscopy reveals highly mobile structures containing enhanced green fluorescent protein-tagged glucose transporter 4 (GLUT4) within a zone about 100 nm beneath the plasma membrane of 3T3-L1 adipocytes. We developed a computer program (Fusion Assistant) that enables direct analysis of the docking/fusion kinetics of hundreds of exocytic fusion events. Insulin stimulation increases the fusion frequency of exocytic GLUT4 vesicles by ∼4-fold, increasing GLUT4 content in the plasma membrane. Remarkably, insulin signaling modulates the kinetics of the fusion process, decreasing the vesicle tethering/docking duration prior to membrane fusion. In contrast, the kinetics of GLUT4 molecules spreading out in the plasma membrane from exocytic fusion sites is unchanged by insulin. As GLUT4 accumulates in the plasma membrane, it is also immobilized in punctate structures on the cell surface. A previous report suggested these structures are exocytic fusion sites (Lizunov et al., J. Cell Biol. 169:481-489, 2005). However, two-color TIRF microscopy using fluorescent proteins fused to clathrin light chain or GLUT4 reveals these structures are clathrin-coated patches. Taken together, these data show that insulin signaling accelerates the transition from docking of GLUT4-containing vesicles to their fusion with the plasma membrane and promotes GLUT4 accumulation in clathrin-based endocytic structures on the plasma membrane.

scholarly journals Visualization of ligand-induced dopamine D2S and D2L receptor internalization by TIRF microscopy

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Alina Tabor ◽  
Dorothee Möller ◽  
Harald Hübner ◽  
Johannes Kornhuber ◽  
Peter Gmeiner
Keyword(s):  
Receptor Internalization ◽  
Tirf Microscopy

scholarly journals Interactions of tropomyosin Tpm1.1 on a single actin filament: A method for extraction and processing of high resolution TIRF microscopy data

PLoS ONE ◽  
2018 ◽  
Vol 13 (12) ◽  
pp. e0208586 ◽  
Author(s):  
Miro Janco ◽  
Till Böcking ◽  
Stanley He ◽  
Adelle C. F. Coster
Keyword(s):  
High Resolution ◽  
Actin Filament ◽  
Tirf Microscopy ◽  
Microscopy Data

scholarly journals Analyzing Receptor Assemblies in the Cell Membrane Using Fluorescence Anisotropy Imaging with TIRF Microscopy

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e100526 ◽  
Author(s):  
Miklos Erdelyi ◽  
Joseph Simon ◽  
Eric A. Barnard ◽  
Clemens F. Kaminski
Keyword(s):  
Cell Membrane ◽  
Fluorescence Anisotropy ◽  
Tirf Microscopy

scholarly journals Methods for Studying Membrane-Associated Bacterial Cytoskeleton Proteins In Vivo by TIRF Microscopy

2019 ◽  
pp. 123-133 ◽  
Author(s):  
Charlène Cornilleau ◽  
Arnaud Chastanet ◽  
Cyrille Billaudeau ◽  
Rut Carballido-López
Keyword(s):  
Tirf Microscopy ◽  
Bacterial Cytoskeleton

Confocal TIRF microscopy of single molecules

2003 ◽  
Author(s):  
Thomas Ruckstuhl ◽  
Michael Rankl ◽  
Stefan Seeger
Keyword(s):  
Single Molecules ◽  
Tirf Microscopy

Elliptical mirror-based TIRF microscopy with shadowless illumination and adjustable penetration depth

2017 ◽  
Vol 42 (13) ◽  
pp. 2587 ◽  
Author(s):  
Jian Liu ◽  
Qiang Li ◽  
Mengzhou Li ◽  
Shan Gao ◽  
Chenguang Liu ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Tirf Microscopy
Sign in / Sign up

Export Citation Format

Share Document