Enzyme Kinetics and Its Analytical Applications

Author(s):  
Brovko Lubov
1968 ◽  
Vol 19 (03/04) ◽  
pp. 364-367 ◽  
Author(s):  
H. C Hemker ◽  
P. W Hemker

SummaryThe enzyme kinetics of competitive inhibition under conditions prevailing in clotting tests are developed and a method is given to measure relative amounts of a competitive inhibitor by means of the t — D plot.


1965 ◽  
Vol 13 (01) ◽  
pp. 155-175 ◽  
Author(s):  
H. C Hemker ◽  
P.W Hemker ◽  
E. A Loeliger

SummaryApplication of the methods of enzyme-kinetic analysis to the results of clotting tests is feasible and can yield useful results. However, the standard methods of enzyme kinetics are not applicable without modifications imposed by the peculiarities of the blood-clotting enzyme system. The influence of the following complicating circumstances is calculated :1. Substrate is not present in excess.2. Only relative measures exist for concentrations of substrate or enzymes.3. Enzymes and substrates are often added together.4. Reagents are not pure.5. Clotting-time is our only measure for clotting-velocity.Formulas are deduced, which makes it possible to recognize the effect of these complications.


2020 ◽  
Vol 63 (7) ◽  
pp. 708-720 ◽  
Author(s):  
V L Vaks ◽  
V A Anfertev ◽  
V Yu Balakirev ◽  
S A Basov ◽  
E G Domracheva ◽  
...  

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 1151-P
Author(s):  
LI CHEN ◽  
YONGQIANG SHAN ◽  
XIAOWEI JIN ◽  
XIAOBING LV

2012 ◽  
Vol 29 (4) ◽  
pp. 440
Author(s):  
Yaqiong WANG ◽  
Shaopu LIU ◽  
Zhongfang LIU ◽  
Xiaoli HU

1983 ◽  
Vol 48 (4) ◽  
pp. 1123-1128
Author(s):  
S. P. Novitskii ◽  
I. I. Burenkov ◽  
V. I. Kenzin ◽  
R. Yu. Beck

Design, principal function and technical parameters of two polarograph-impendancemeters are given. The instruments are suitable both for analytical applications and for investigation of the kinetics and mechanism of electrode processes.


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