A balanced, phase sensitive back-focal plane interferometry technique to determine dynamics of a trapped bead in optical tweezers

2012 ◽  
Author(s):  
Basudev Roy ◽  
Sambit Bikas Pal ◽  
Arijit Haldar ◽  
Ratnesh Kumar Gupta ◽  
Nirmalya Ghosh ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Focal Plane ◽  
Phase Sensitive

Extended linear detection range for optical tweezers using a stop at the back focal plane of the condenser

2015 ◽  
Vol 17 (6) ◽  
pp. 065606 ◽  
Author(s):  
S Masoumeh Mousavi ◽  
Akbar Samadi ◽  
Faegheh Hajizadeh ◽  
S Nader S Reihani
Keyword(s):  
Optical Tweezers ◽  
Focal Plane ◽  
Detection Range ◽  
Linear Detection

Using back focal plane interferometry to probe the influence of Zernike aberrations in optical tweezers

2017 ◽  
Vol 42 (15) ◽  
pp. 2968 ◽  
Author(s):  
Thomas F. Dixon ◽  
Lachlan W. Russell ◽  
Ana Andres-Arroyo ◽  
Peter J. Reece
Keyword(s):  
Optical Tweezers ◽  
Focal Plane

Enhancement of axial force of optical tweezers by utilizing a circular stop at the back focal plane of the objective

2018 ◽  
Vol 35 (11) ◽  
pp. 2654 ◽  
Author(s):  
Hossein Gorjizadeh Alinezhad ◽  
Sajad Meydanloo ◽  
S. Nader S. Reihani
Keyword(s):  
Optical Tweezers ◽  
Axial Force ◽  
Focal Plane

scholarly journals Holographic optical tweezers combined with back-focal-plane displacement detection

2013 ◽  
Vol 21 (25) ◽  
pp. 30282 ◽  
Author(s):  
Ferran Marsà ◽  
Arnau Farré ◽  
Estela Martín-Badosa ◽  
Mario Montes-Usategui
Keyword(s):  
Optical Tweezers ◽  
Focal Plane ◽  
Holographic Optical Tweezers ◽  
Plane Displacement

High Resolution Two-Dimensional Detection of Ncd Motility with Optical Tweezers

1997 ◽  
Vol 489 ◽  
Author(s):  
M. W. Allersma ◽  
F. Gittes ◽  
M. J. deCastro ◽  
R. J. Stewart ◽  
C. F. Schmidt
Keyword(s):  
Optical Tweezers ◽  
Focal Plane ◽  
Detection Method ◽  
Field Of View ◽  
Viscous Drag ◽  
Two Dimensional ◽  
Laser Focus ◽  
Temporal And Spatial ◽  
Elastic Constraints

AbstractThe ATP-dependent motility of the kinesin-related non claret disjunctional (ncd) mechanoenzyme was observed in an in vitro bead motility assay using optical tweezers in combination with a new two-dimensional displacement detection method. The detection technique is based on observing the far-field interference pattern formed in the back focal plane (BFP) of the microscope condenser by the illuminating laser focus and the light scattered from the trapped dielectric bead. The ability to observe the two-dimensional motion, with high temporal and spatial resolution, and in a manner largely independent of position in the microscope field-of-view, is the particular advantage of this detection method. In the assay, a fusion protein (GST-N195) of truncated ncd and glutathione-Stransferase was adsorbed to silica beads and the axial and lateral motions of the beads along the microtubule surface were observed. The average axial velocity of the ncd coated beads was 230 ± 30 nm/s (± std. dev.). Spectral analysis of bead motion showed an increase in viscous drag near the surface. Furthermore, we also found that any elastic constraints of the moving motors are much smaller than the constraints due to binding in the presence of the non-hydrolyzable nucleotide adenylylimido-diphosphate (AMP-PNP).

scholarly journals Probing the dynamics of an optically trapped particle by phase sensitive back focal plane interferometry

2012 ◽  
Vol 20 (8) ◽  
pp. 8317 ◽  
Author(s):  
Basudev Roy ◽  
Sambit Bikas Pal ◽  
Arijit Haldar ◽  
Ratnesh Kumar Gupta ◽  
Nirmalya Ghosh ◽  
...  
Keyword(s):  
Focal Plane ◽  
Phase Sensitive ◽  
Optically Trapped

scholarly journals Calibration of optical tweezers with positional detection in the back focal plane

2006 ◽  
Vol 77 (10) ◽  
pp. 103101 ◽  
Author(s):  
Simon F. Tolić-Nørrelykke ◽  
Erik Schäffer ◽  
Jonathon Howard ◽  
Francesco S. Pavone ◽  
Frank Jülicher ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Focal Plane
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