In vitro quantitation of human femoral artery atherosclerosis using near-infrared Raman spectroscopy

2012 ◽  
Author(s):  
Ava C. Dykes ◽  
Pavlos Anastasiadis ◽  
John S. Allen ◽  
Shiv K. Sharma
Keyword(s):  
Raman Spectroscopy ◽  
Femoral Artery ◽  
Near Infrared

scholarly journals Identification of hepatitis C in human blood serum by near-infrared Raman spectroscopy

2008 ◽  
Vol 22 (5) ◽  
pp. 387-395 ◽  
Author(s):  
Jamil Saade ◽  
Marcos Tadeu Tavares Pacheco ◽  
Maira Regina Rodrigues ◽  
Landulfo Silveira Jr.
Keyword(s):  
Raman Spectroscopy ◽  
Hepatitis C ◽  
Blood Serum ◽  
Human Blood ◽  
Near Infrared ◽  
Viral Infections ◽  
Human Blood Serum ◽  
Biochemical Alterations ◽  
Viral Hepatitis C

Hepatitis C has become one of the higher health problems around the world. Near-infrared Raman spectroscopy had been used to spectrally differentiate among health human blood serum from the one with hepatitis C contaminationin vitro. In this study a Raman spectrometer with 80 mW, 830 nm excitation, liquid-nitrogen cooled CCD and imaging spectrograph were used to collect Raman scattering from 24 blood samples (14 healthy and 10 diseased) with collection time of 120 s. It has been used an algorithm based on the Principal Components Analysis (PCA) for main spectral features identification and Mahalanobis distance for blood spectrum classification depending on the serology. It was observed that the highest spectral differences between the two types of human blood serum were found in 1002, 1169, 1262 and 1348 cm−1Raman bands. The spectral analysis using multivariate statistics presented good results when compared to classical diagnosis for viral hepatitis C, showing that Raman spectroscopy can classify human blood serum spectrum in one of the two categories by identifying biochemical alterations that occur in the presence of viral infections.

scholarly journals Application of FTIR and Raman Spectroscopy to Characterisation of Bioactive Materials and Living Cells

2003 ◽  
Vol 17 (2-3) ◽  
pp. 275-288 ◽  
Author(s):  
I. Notingher ◽  
J. R. Jones ◽  
S. Verrier ◽  
I. Bisson ◽  
P. Embanga ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Near Infrared ◽  
Cell Damage ◽  
Living Cells ◽  
Biologically Active ◽  
Bioactive Materials ◽  
45S5 Bioglass ◽  
Ftir And Raman Spectroscopy

Both Fourier Transform Infrared (FTIR) and Raman spectroscopy have been applied to thein vitrocharacterisation of biomaterials, mainly surface reactions leading to the formation of a biologically active hydroxycarbonate apatite (HCA) layer on the sample surface when immersed in simulated body fluids (SBF). The HCA layer indicates the degree of bioactivity of the sample, because it leads to a strong bond between the biomaterial and living tissue. Reflection measurements using FTIR allow quick, non-destructive detection of the HCA layer for solid and powder samples. Due to the low Raman scattering efficiency and low absorption of water in the visible-near infrared region, Raman micro-spectroscopy was successfully used for thein situcharacterisation of 20 and 40µm diameter 45S5 Bioglass®fibres. Thein situcapabilities of the Raman micro-spectrometer have also been extended to the characterisation of living cells attached on bioinert silica and bioactive 45S5 Bioglass®and 58S substrates. Using a high power 785 nm laser, living cells in physiological conditions can be real-time sampled over long periods of time without inducing cell damage and with good signal strength. Cell death can be monitored because it proved to induce strong changes in the Raman signature in the spectral regions 1000–1150 cm–1and 1550–1650 cm–1.

Near-Infrared Raman Spectroscopy for In Vitro Detection of Cervical Precancers

1998 ◽  
Vol 68 (1) ◽  
pp. 123-132 ◽  
Author(s):  
Anita Mahadevan-Jansen ◽  
Michele Follen Mitchell ◽  
Nirmala Ramanujamf ◽  
Anais Malpica ◽  
Sharon Thomsen ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Near Infrared

scholarly journals Near-Infrared Micro-Raman Spectroscopy for in Vitro Detection of Cervical Cancer

2010 ◽  
Vol 64 (3) ◽  
pp. 255-261 ◽  
Author(s):  
Lori E. Kamemoto ◽  
Anupam K. Misra ◽  
Shiv K. Sharma ◽  
Marc T. Goodman ◽  
Hugh Luk ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Raman Spectroscopy ◽  
Near Infrared ◽  
Micro Raman Spectroscopy

scholarly journals Comparative analysis of mouse bone marrow and adipose tissue mesenchymal stem cells for critical limb ischemia cell therapy

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Pegah Nammian ◽  
Seyedeh-Leili Asadi-Yousefabad ◽  
Sajad Daneshi ◽  
Mohammad Hasan Sheikhha ◽  
Seyed Mohammad Bagher Tabei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cell Therapy ◽  
Femoral Artery ◽  
Critical Limb Ischemia ◽  
Limb Ischemia

Abstract Introduction Critical limb ischemia (CLI) is the most advanced form of peripheral arterial disease (PAD) characterized by ischemic rest pain and non-healing ulcers. Currently, the standard therapy for CLI is the surgical reconstruction and endovascular therapy or limb amputation for patients with no treatment options. Neovasculogenesis induced by mesenchymal stem cells (MSCs) therapy is a promising approach to improve CLI. Owing to their angiogenic and immunomodulatory potential, MSCs are perfect candidates for the treatment of CLI. The purpose of this study was to determine and compare the in vitro and in vivo effects of allogeneic bone marrow mesenchymal stem cells (BM-MSCs) and adipose tissue mesenchymal stem cells (AT-MSCs) on CLI treatment. Methods For the first step, BM-MSCs and AT-MSCs were isolated and characterized for the characteristic MSC phenotypes. Then, femoral artery ligation and total excision of the femoral artery were performed on C57BL/6 mice to create a CLI model. The cells were evaluated for their in vitro and in vivo biological characteristics for CLI cell therapy. In order to determine these characteristics, the following tests were performed: morphology, flow cytometry, differentiation to osteocyte and adipocyte, wound healing assay, and behavioral tests including Tarlov, Ischemia, Modified ischemia, Function and the grade of limb necrosis scores, donor cell survival assay, and histological analysis. Results Our cellular and functional tests indicated that during 28 days after cell transplantation, BM-MSCs had a great effect on endothelial cell migration, muscle restructure, functional improvements, and neovascularization in ischemic tissues compared with AT-MSCs and control groups. Conclusions Allogeneic BM-MSC transplantation resulted in a more effective recovery from critical limb ischemia compared to AT-MSCs transplantation. In fact, BM-MSC transplantation could be considered as a promising therapy for diseases with insufficient angiogenesis including hindlimb ischemia.

scholarly journals Fibroblast activation protein targeted near infrared photoimmunotherapy (NIR PIT) overcomes therapeutic resistance in human esophageal cancer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ryoichi Katsube ◽  
Kazuhiro Noma ◽  
Toshiaki Ohara ◽  
Noriyuki Nishiwaki ◽  
Teruki Kobayashi ◽  
...  
Keyword(s):  
Esophageal Cancer ◽  
Cancer Cells ◽  
Cancer Progression ◽  
Near Infrared ◽  
Fibroblast Activation Protein ◽  
Therapeutic Resistance ◽  
Fibroblast Activation ◽  
Human Esophageal Cancer

AbstractCancer-associated fibroblasts (CAFs) have an important role in the tumor microenvironment. CAFs have the multifunctionality which strongly support cancer progression and the acquisition of therapeutic resistance by cancer cells. Near-infrared photoimmunotherapy (NIR-PIT) is a novel cancer treatment that uses a highly selective monoclonal antibody (mAb)-photosensitizer conjugate. We developed fibroblast activation protein (FAP)-targeted NIR-PIT, in which IR700 was conjugated to a FAP-specific antibody to target CAFs (CAFs-targeted NIR-PIT: CAFs-PIT). Thus, we hypothesized that the control of CAFs could overcome the resistance to conventional chemotherapy in esophageal cancer (EC). In this study, we evaluated whether EC cell acquisition of stronger malignant characteristics and refractoriness to chemoradiotherapy are mediated by CAFs. Next, we assessed whether the resistance could be rescued by eliminating CAF stimulation by CAFs-PIT in vitro and in vivo. Cancer cells acquired chemoradiotherapy resistance via CAF stimulation in vitro and 5-fluorouracil (FU) resistance in CAF-coinoculated tumor models in vivo. CAF stimulation promoted the migration/invasion of cancer cells and a stem-like phenotype in vitro, which were rescued by elimination of CAF stimulation. CAFs-PIT had a highly selective effect on CAFs in vitro. Finally, CAF elimination by CAFs-PIT in vivo demonstrated that the combination of 5-FU and NIR-PIT succeeded in producing 70.9% tumor reduction, while 5-FU alone achieved only 13.3% reduction, suggesting the recovery of 5-FU sensitivity in CAF-rich tumors. In conclusion, CAFs-PIT could overcome therapeutic resistance via CAF elimination. The combined use of novel targeted CAFs-PIT with conventional anticancer treatments can be expected to provide a more effective and sensible treatment strategy.

scholarly journals Applications of Vibrational Spectroscopy for Analysis of Connective Tissues

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 922
Author(s):  
William Querido ◽  
Shital Kandel ◽  
Nancy Pleshko
Keyword(s):  
Raman Spectroscopy ◽  
Vibrational Spectroscopy ◽  
Near Infrared ◽  
Ex Vivo ◽  
Biological Tissues ◽  
Label Free ◽  
Connective Tissues ◽  
Bone Properties ◽  
Composition And Structure

Advances in vibrational spectroscopy have propelled new insights into the molecular composition and structure of biological tissues. In this review, we discuss common modalities and techniques of vibrational spectroscopy, and present key examples to illustrate how they have been applied to enrich the assessment of connective tissues. In particular, we focus on applications of Fourier transform infrared (FTIR), near infrared (NIR) and Raman spectroscopy to assess cartilage and bone properties. We present strengths and limitations of each approach and discuss how the combination of spectrometers with microscopes (hyperspectral imaging) and fiber optic probes have greatly advanced their biomedical applications. We show how these modalities may be used to evaluate virtually any type of sample (ex vivo, in situ or in vivo) and how “spectral fingerprints” can be interpreted to quantify outcomes related to tissue composition and quality. We highlight the unparalleled advantage of vibrational spectroscopy as a label-free and often nondestructive approach to assess properties of the extracellular matrix (ECM) associated with normal, developing, aging, pathological and treated tissues. We believe this review will assist readers not only in better understanding applications of FTIR, NIR and Raman spectroscopy, but also in implementing these approaches for their own research projects.

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