Development of a phase modulation surface plasmon resonance image detection system based on time-stepped quadrature phase shifting method

2012 ◽  
Author(s):  
Shu-Sheng Lee ◽  
Tsung-Yen Huang ◽  
Sheng-En Chen ◽  
Chih-Chieh Hung
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Phase Modulation ◽  
Detection System ◽  
Phase Shifting ◽  
Image Detection ◽  
Quadrature Phase ◽  
Phase Shifting Method

A surface plasmon resonance assay coupled with a hybridization chain reaction for amplified detection of DNA and small molecules

2014 ◽  
Vol 50 (39) ◽  
pp. 5049-5052 ◽  
Author(s):  
Xuemei Li ◽  
Yan Wang ◽  
Linlin Wang ◽  
Qingli Wei
Keyword(s):  
Surface Plasmon Resonance ◽  
Small Molecules ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Biological Samples ◽  
Detection System ◽  
High Sensitivity ◽  
Hybridization Chain Reaction ◽  
Chain Reaction

A surface plasmon resonance (SPR) detection system based on a hybridization chain reaction (HCR) was developed for amplified detection of DNA and small molecules with high sensitivity. This methodology is capable of detecting the target in complicated biological samples and can be further extended to the detection of other proteins or biomarkers.

scholarly journals Surface Plasmon Resonance-Based Sensing Utilizing Spatial Phase Modulation in an Imaging Interferometer

Sensors ◽  
2020 ◽  
Vol 20 (6) ◽  
pp. 1616
Author(s):  
Roman Kaňok ◽  
Dalibor Ciprian ◽  
Petr Hlubina
Keyword(s):  
Refractive Index ◽  
Surface Plasmon Resonance ◽  
Detection Limit ◽  
Phase Shift ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Phase Modulation ◽  
Spatial Phase ◽  
Plasmonic Structure ◽  
Imaging Interferometer

Spatial phase modulation in an imaging interferometer is utilized in surface plasmon resonance (SPR) based sensing of liquid analytes. In the interferometer, a collimated light beam from a laser diode irradiating at 637.1 nm is passing through a polarizer and is reflected from a plasmonic structure of SF10/Cr/Au attached to a prism in the Kretschmann configuration. The beam passes through a combination of a Wollaston prism, a polarizer and a lens, and forms an interference pattern on a CCD sensor of a color camera. Interference patterns obtained for different liquid analytes are acquired and transferred to the computer for data processing. The sensing concept is based on the detection of a refractive index change, which is transformed via the SPR phenomenon into an interference fringe phase shift. By calculating the phase shift for the plasmonic structure of SF10/Cr/Au of known parameters we demonstrate that this technique can detect different weight concentrations of ethanol diluted in water, or equivalently, different changes in the refractive index. The sensitivity to the refractive index and the detection limit obtained are −278 rad/refractive-index-unit (RIU) and 3.6 × 10 − 6 RIU, respectively. The technique is demonstrated in experiments with the same liquid analytes as in the theory. Applying an original approach in retrieving the fringe phase shift, we revealed good agreement between experiment and theory, and the measured sensitivity to the refractive index and the detection limit reached −226 rad/RIU and 4.4 × 10 − 6 RIU, respectively. These results suggest that the SPR interferometer with the detection of a fringe phase shift is particularly useful in applications that require measuring refractive index changes with high sensitivity.

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