Tubular optical waveguide-based particle plasmon resonance biosensor for label-free and real-time detection

2012 ◽  
Author(s):  
Hsing-Ying Lin ◽  
Chen-Han Huang ◽  
Yu-Chia Liu ◽  
Shin-Huei Chen ◽  
Lai-Kwan Chau
Keyword(s):  
Real Time ◽  
Optical Waveguide ◽  
Plasmon Resonance ◽  
Label Free ◽  
Real Time Detection ◽  
Particle Plasmon

scholarly journals Molecular inversion probe-based SPR biosensing for specific, label-free and real-time detection of regional DNA methylation

2014 ◽  
Vol 50 (27) ◽  
pp. 3585-3588 ◽  
Author(s):  
Laura G. Carrascosa ◽  
Abu Ali Ibn Sina ◽  
Ramkumar Palanisamy ◽  
Borja Sepulveda ◽  
Marinus A. Otte ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Label Free ◽  
Real Time Detection ◽  
Molecular Inversion Probe ◽  
Specific Label

DNA regional methylation can be detected in real-time and label-free using surface plasmon resonance biosensing coupled to molecular inversion probe based amplification.

scholarly journals Fiber Optic Particle Plasmon Resonance Biosensor for Label-Free Detection of Nucleic Acids and Its Application to HLA-B27 mRNA Detection in Patients with Ankylosing Spondylitis

Sensors ◽  
2020 ◽  
Vol 20 (11) ◽  
pp. 3137
Author(s):  
Yen-Ta Tseng ◽  
Wan-Yun Li ◽  
Ya-Wen Yu ◽  
Chang-Yue Chiang ◽  
Su-Qin Liu ◽  
...  
Keyword(s):  
Ankylosing Spondylitis ◽  
Nucleic Acids ◽  
Real Time ◽  
Plasmon Resonance ◽  
Fiber Optic ◽  
Hla B27 ◽  
Label Free ◽  
Mrna Detection ◽  
Label Free Detection ◽  
Particle Plasmon

We developed a label-free, real-time, and highly sensitive nucleic acid biosensor based on fiber optic particle plasmon resonance (FOPPR). The biosensor employs a single-strand deoxyoligonucleotides (ssDNA) probe, conjugated to immobilized gold nanoparticles on the core surface of an optical fiber. We explore the steric effects on hybridization affinity and limit of detection (LOD), by using different ssDNA probe designs and surface chemistries, including diluent molecules of different lengths in mixed self-assembled monolayers, ssDNA probes of different oligonucleotide lengths, ssDNA probes in different orientations to accommodate target oligonucleotides with a hybridization region located unevenly in the strand. Based on the optimized ssDNA probe design and surface chemistry, we achieved LOD at sub-nM level, which makes detection of target oligonucleotides as low as 1 fmol possible in the 10-μL sensor chip. Additionally, the FOPPR biosensor shows a good correlation in determining HLA-B27 mRNA, in extracted blood samples from patients with ankylosing spondylitis (AS), with the clinically accepted real-time reverse transcription-polymerase chain reaction (RT-PCR) method. The results from this fundamental study should guide the design of ssDNA probe for anti-sense sensing. Further results through application to HLA-B27 mRNA detection illustrate the feasibility in detecting various nucleic acids of chemical and biological relevance.

Label-Free and Regenerable Aptasensor for Real-Time Detection of Cadmium(II) by Dual Polarization Interferometry

2020 ◽  
Vol 92 (14) ◽  
pp. 10007-10015 ◽  
Author(s):  
Yu Xue ◽  
Yu Wang ◽  
Shuang Wang ◽  
Mengxia Yan ◽  
Jianshe Huang ◽  
...  
Keyword(s):  
Real Time ◽  
Label Free ◽  
Dual Polarization ◽  
Polarization Interferometry ◽  
Dual Polarization Interferometry ◽  
Real Time Detection

Real-time detection of the chemokine CXCL12 in urine samples by surface plasmon resonance

Talanta ◽  
2013 ◽  
Vol 109 ◽  
pp. 209-215 ◽  
Author(s):  
Beatriz Vega ◽  
Ana Calle ◽  
Alejandra Sánchez ◽  
Laura M. Lechuga ◽  
Ana M. Ortiz ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Urine Samples ◽  
Chemokine Cxcl12 ◽  
Real Time Detection
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